Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 4,759,531 G→T A3S (GCC→TCC)  ligM_KToptimized → sequence generated by OPTIMIZER for KT2440

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,759,5310GT98.4% 201.4 / NA 63A3S (GCC→TCC) ligM_KToptimizedsequence generated by OPTIMIZER for KT2440
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (41/21);  total (41/22)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGG  >  ACB143/4759400‑4759662
                                                                                                                                   |                                                                                                                                   
aCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTcccc                                                                                                                                 <  6:137403/136‑1 (MQ=255)
aCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTcccc                                                                                                                                 <  3:304769/136‑1 (MQ=255)
                ccAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTACGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAg                                                                                                                 >  5:284441/1‑136 (MQ=255)
                 cAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAgg                                                                                                                >  2:219860/1‑136 (MQ=255)
                 cAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAgg                                                                                                                >  7:137398/1‑136 (MQ=255)
                         gattttttAGCTGTTGAGAATTAATCATCTGCTCGTATAATGTGTGGTATTGTGTGCGGATAACAATTTCACACCAACACATATGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGTTGCTGgcc                                                                                                        <  4:322530/134‑1 (MQ=25)
                                gAGCTGTTTAAAATTAATCATCGTGTCGTA‑AATGTGTGTAATTGTGAGCGGATAACAATTTCACACCAACAGATATGGTTTAGGAGGTATACATGAGCTCCCCGTCTAAGCTGGAGCAGGTGCTGGCCGCcgggg                                                                                                 <  6:54224/135‑3 (MQ=25)
                                gAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCcggc                                                                                                  <  2:309992/135‑1 (MQ=255)
                                 aGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCcggcgg                                                                                                <  1:284609/136‑1 (MQ=255)
                                 aGCTGTTGACAATAAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCcggcgg                                                                                                <  6:267091/136‑1 (MQ=255)
                                   cTGTTGACAATTAATCATCGGCTCGTTTAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATCGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCa                                                                                              <  2:206700/136‑1 (MQ=255)
                                      ttGACAATTACTCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCaa                                                                                             <  8:252355/134‑1 (MQ=255)
                                                 tcatcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATg                                                                                >  5:151441/1‑136 (MQ=255)
                                                   atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGtt                                                                              >  1:114590/1‑136 (MQ=255)
                                                   atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGtt                                                                              >  1:318137/1‑136 (MQ=255)
                                                    tcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTg                                                                             <  5:141821/136‑1 (MQ=255)
                                                          cGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAAc                                                                       >  2:236963/1‑136 (MQ=255)
                                                                     tgGAATTGTGAGCGGATAACAATTTCACACCACCACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTgg                                                            >  2:104806/1‑136 (MQ=255)
                                                                      ggAATTGTGCCCGTATAACAATTTAACACCAACACATAGGGTTTAGTAGGTATACTTGAGCTCCCCGACTAACGTGGAGCAGGTGCTGGCGGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCGAGATTGgc                                                           <  3:277442/136‑1 (MQ=255)
                                                                       gAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgc                                                           <  1:177284/135‑1 (MQ=255)
                                                                        aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACCCCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                                         >  7:283971/1‑136 (MQ=255)
                                                                        aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                                         >  4:70523/1‑136 (MQ=255)
                                                                        aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                                         >  6:133022/1‑136 (MQ=255)
                                                                        aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                                         >  5:289120/1‑136 (MQ=255)
                                                                        aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                                         >  1:193012/1‑136 (MQ=255)
                                                                        aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                                         >  3:135512/1‑136 (MQ=255)
                                                                        aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                                         >  4:293410/1‑136 (MQ=255)
                                                                        aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                                         >  4:256576/1‑136 (MQ=255)
                                                                        aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                                         >  7:60696/1‑136 (MQ=255)
                                                                         aTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGcc                                                        >  6:11692/1‑136 (MQ=255)
                                                                                gCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTc                                                  >  3:124394/1‑135 (MQ=255)
                                                                                gCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTc                                                  >  7:181160/1‑135 (MQ=255)
                                                                                  ggATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTg                                                                             <  6:202757/106‑1 (MQ=255)
                                                                                  ggATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTg                                                                             >  5:202757/1‑106 (MQ=255)
                                                                                  ggATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTAc                                               >  6:250217/1‑136 (MQ=255)
                                                                                       aCAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgt                                          <  6:151441/136‑1 (MQ=255)
                                                                                         aaTTTCACACCAACACATAGTGTTTAGCCGGTATACATGAGCTCCCCGACTATCCTGTAGCAGGTGCTGGACGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgtcg                                        <  8:64335/136‑1 (MQ=255)
                                                                                         aaTTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgtcg                                        <  7:247753/136‑1 (MQ=255)
                                                                                          aTTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgtcgt                                       >  4:215477/1‑136 (MQ=255)
                                                                                                cacCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGcc                                 <  6:123908/136‑1 (MQ=255)
                                                                                                 acCAACACATAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCa                                >  5:72988/1‑136 (MQ=255)
                                                                                                       acaTAGGGTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTtc                          <  7:265655/136‑1 (MQ=255)
                                                                                                            gggTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGCAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa                     <  6:113805/136‑1 (MQ=255)
                                                                                                              gTTTAGGAGGTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACt                   >  6:56862/1‑136 (MQ=255)
                                                                                                                  aggcggTAAACATGCGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCTCTCAATAGTTCTCTAACTGGc                <  3:239280/135‑1 (MQ=255)
                                                                                                                    gaggTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa             >  5:167527/1‑136 (MQ=255)
                                                                                                                    gaggTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa             >  5:82088/1‑136 (MQ=255)
                                                                                                                    gaggTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa             >  7:38855/1‑136 (MQ=255)
                                                                                                                     aggTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTAc            >  8:230988/1‑136 (MQ=255)
                                                                                                                     aggTATACATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTAc            >  8:170055/1‑136 (MQ=255)
                                                                                                                          taCATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa        >  1:137353/1‑135 (MQ=255)
                                                                                                                          taCATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa        >  1:56328/1‑135 (MQ=255)
                                                                                                                          taCATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa        >  4:93464/1‑135 (MQ=255)
                                                                                                                          taCATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa        >  7:247844/1‑135 (MQ=255)
                                                                                                                          taCATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa        >  4:219130/1‑135 (MQ=255)
                                                                                                                          taCATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa        >  1:64974/1‑135 (MQ=255)
                                                                                                                          taCATGAGCTCCCCGACTAACCTGGAGCAGGTGCGGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAAc       <  7:66020/136‑1 (MQ=255)
                                                                                                                          taCATGAGCTCACCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAAAGTTGCGTAACTCCCAGATTGGAGCCTACGTCTACCCGGTCGTCGCGCCAGAGATCTCTAACTGGCGTACCGAAc       >  3:17117/1‑136 (MQ=255)
                                                                                                                           aCATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGac        >  6:26287/1‑133 (MQ=255)
                                                                                                                           aCATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa        >  7:16858/1‑134 (MQ=255)
                                                                                                                           aCATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa        >  4:218496/1‑134 (MQ=255)
                                                                                                                            cATGAGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAg     >  8:277272/1‑136 (MQ=255)
                                                                                                                                aGCTCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTgg  <  5:41048/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                   
ACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGG  >  ACB143/4759400‑4759662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑