| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,386,506 | 1 bp→GT | intergenic (‑515/+1136) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,505 | 1 | . | G | 81.0% | 60.5 / 7.8 | 21 | intergenic (‑514/+1137) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/4); new base G (7/10); total (7/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | ACB143 | 5,386,506 | 0 | C | T | 85.0% | 32.3 / ‑0.8 | 21 | intergenic (‑515/+1136) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (7/10); minor base A (0/3); total (7/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑CGT‑‑CGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGG > ACB143/5386422‑5386604 || aTAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGAt < 7:26370/135‑1 (MQ=255) aaaTTGATCGAATTCTTTCATTTACGACCCTAATATGACATCTCGGCCGGCTGGTCGACGACGGCGGGCTC‑‑CGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc < 7:167457/136‑1 (MQ=255) aaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc < 1:119058/136‑1 (MQ=255) aTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCGCGGCCGGCTTGTCGACGACGGCGGGCTC‑‑AGTGGGGT‑AC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg < 5:68628/136‑1 (MQ=255) aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg < 3:171180/135‑1 (MQ=255) tCATTTACGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt < 8:196016/134‑1 (MQ=255) aTTTAAGACCCTAATATGACATCTCGGCCGGCTTGGCGTCGACGGCGGTCTCATTGG‑‑GGT‑AC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCGCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 4:211001/136‑1 (MQ=255) aTTAAACACCCTAATATGACAGCTCGGCCGGCTGGGCGACGACGGCGGGCTCAGGGG‑‑GGT‑AC‑GGCAC‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 6:211020/136‑1 (MQ=255) tttAAGACCCTAATATGACATCTCGGCCGGCGTGGCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCt < 6:102944/136‑1 (MQ=255) tAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTg < 8:102916/135‑1 (MQ=255) aCCCAAACATGACCTCTCGGCCGGCTGGCCGACGACGGCGGTCTC‑‑CGT‑‑GGTGTACGGAACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt < 5:206403/136‑1 (MQ=255) atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 1:252428/136‑1 (MQ=255) atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc > 7:127590/1‑136 (MQ=255) atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc > 2:266125/1‑136 (MQ=255) tGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGTGGTGT‑CC‑GGCACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc < 1:258104/135‑1 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 4:73429/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 7:326557/1‑136 (MQ=255) tcGGCCGGCTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt > 8:189126/1‑136 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg > 4:323933/1‑136 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg > 3:319361/1‑136 (MQ=255) ggccggcGTGTCGACGACGGCGGTCTC‑‑AGT‑‑GGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGccc < 7:14793/132‑1 (MQ=255) || ATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTC‑‑CGT‑‑CGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGG > ACB143/5386422‑5386604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |