Predicted mutation
evidence seq id position mutation freq annotation gene description
JC ACB143 5,389,996 Δ8 bp 100% coding (90‑97/1666 nt) ligH → 10‑formyltetrahydrofolate synthetase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 = 53899951 (0.020)32 (0.730) 23/242 NT 97.0% coding (89/1666 nt) ligH 10‑formyltetrahydrofolate synthetase
?ACB143 5390004 = 1 (0.020)coding (98/1666 nt) ligH 10‑formyltetrahydrofolate synthetase

CGACCTGCCCGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/5389865‑5389995
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACC  >  ACB143/5390004‑5390134
                                                                                                                                                                                                                                                                      
cccccgGCCGGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAAC                                                                                                                                <  2:122690/130‑1
 GACCTGCCCGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAAC                                                                                                                                >  5:84624/1‑135
  ACCTGCCCGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGCAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTA                                                                                                                              >  1:239068/1‑136
   CCTGCCGGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCGGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTAC                                                                                                                             <  8:171838/136‑1
   CCTGCCCGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTAC                                                                                                                             <  5:220747/136‑1
    cgGCCGGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACG                                                                                                                            <  3:167016/134‑1
          GGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATTAACTACGGCCCCT                                                                                                                      <  8:188150/136‑1
          GGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCT                                                                                                                      <  7:23155/136‑1
             CTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACA                                                                                                                   <  2:93519/136‑1
               CGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAG                                                                                                                 <  8:128762/136‑1
                   ACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCA                                                                                                             <  7:63027/136‑1
                    CGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAA                                                                                                            <  3:270269/136‑1
                    CGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAA                                                                                                            <  3:104156/136‑1
                    CGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCC                                                                                                              >  8:43953/1‑134
                        AAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCT                                                                                                         >  3:65325/1‑135
                                        ACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTATGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCA                                                                                        <  6:127254/136‑1
                                        ACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCA                                                                                        <  1:110013/136‑1
                                        ACATCGCACAAGTCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGACCTACGGCCCCTACACGGCCAAGCTGAGCTGGGACTTCATCA                                                                                        <  7:101811/136‑1
                                                  AGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTT                                                                                >  6:46477/1‑134
                                                           TATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGCGAATAAATCAACAGCGTTCAGGGCAAC                                                                       >  5:270905/1‑134
                                                              CGAGATCGGCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGA                                                                  <  8:271913/136‑1
                                                                   TCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCA                                                             <  8:42588/136‑1
                                                                                       gAGAATATCGCGGAGGTGGGGTAGAAAGTCGGCATCGCGCAGGATGAACTACGGCCCCTAAAAGGCCAAGCTTAGCTGGTACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCTTCACCGC                                          <  6:270905/134‑1
                                                                                       CAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGC                                          <  5:269299/135‑1
                                                                                          AATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGAT                                       >  6:84647/1‑135
                                                                                                  GGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGAC                              >  3:271753/1‑136
                                                                                                      GTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCC                           <  3:2463/135‑1
                                                                                                          GCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCG                      <  1:229060/136‑1
                                                                                                          GCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGCCCAAGCTGAGCTGCGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCG                      <  6:57396/136‑1
                                                                                                                     GGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAA            <  7:118239/135‑1
                                                                                                                          CCCGCAGGATTAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCAC      <  5:109583/136‑1
                                                                                                                          CCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCTCTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCAC      <  5:199642/136‑1
                                                                                                                           CCGCATGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCGGACGGCGGCCGGCGAAGGCAAGACCAC      <  7:71837/135‑1
                                                                                                                             GCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCCCCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGAC   >  3:233491/1‑136
                                                                                                                              CAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACC  <  2:239068/136‑1
                                                                                                                              CAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACC  <  1:263503/136‑1
                                                                                                                                                                                                                                                                      
CGACCTGCCCGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/5389865‑5389995
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACC  >  ACB143/5390004‑5390134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.