Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,026,049 T→A 6.0% L346M (TTG→ATG)  nylB → 6‑aminohexanoate‑dimer hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,026,0490TA 6.0% 240.7 / 2.5 74L346M (TTG→ATG) nylB6‑aminohexanoate‑dimer hydrolase
Reads supporting (aligned to +/- strand):  ref base T (31/38);  new base A (3/2);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGGCCCAGTGCACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGGCGCTGGCGGACTTCCCCGGCATACTCTCGGC  >  ACB143/1025915‑1026184
                                                                                                                                      |                                                                                                                                       
ctgggCCCAGTGCACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAAtt                                                                                                                                        <  3:167141/133‑1 (MQ=255)
ctgggCCCAGTGCACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAAtt                                                                                                                                        <  3:163277/133‑1 (MQ=255)
cTTGGCCCAGTGCACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAAtt                                                                                                                                        <  3:90886/136‑1 (MQ=255)
   ggCCCAGTGCACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGc                                                                                                                                      <  4:10167/135‑1 (MQ=255)
     cccAGTGCACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCa                                                                                                                                   <  4:264479/136‑1 (MQ=255)
       cAGTGCACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAg                                                                                                                                 <  1:71228/136‑1 (MQ=255)
       cAGTGCACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAg                                                                                                                                 <  7:90476/136‑1 (MQ=255)
            cACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTCCGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGAGGGt                                                                                                                            >  2:150178/1‑136 (MQ=255)
            cACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGt                                                                                                                            >  6:101875/1‑136 (MQ=255)
            cACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGt                                                                                                                            >  5:60132/1‑136 (MQ=255)
            cACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAAATGCTCAAGCGGGt                                                                                                                            >  4:84753/1‑136 (MQ=255)
            cACCGGCAGACACCTTCGCCGCCCTTCGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGt                                                                                                                            >  4:11699/1‑136 (MQ=255)
              ccGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGc                                                                                                                          <  2:122642/136‑1 (MQ=255)
              ccGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGc                                                                                                                          <  2:253596/136‑1 (MQ=255)
              ccGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGc                                                                                                                          <  6:38333/136‑1 (MQ=255)
                            cgccgcCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGcc                                                                                                            >  5:175687/1‑136 (MQ=255)
                               cgccCGTGGCCACGGGGGGCAGGCTTTATATGTGCTGCCTGCGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCggg                                                                                                         <  2:150173/136‑1 (MQ=255)
                                         cACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCAt                                                                                               <  7:38203/136‑1 (MQ=255)
                                               gggCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCg                                                                                         >  7:206408/1‑136 (MQ=255)
                                                 gCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGc                                                                                                                          <  7:149006/101‑1 (MQ=255)
                                                 gCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGc                                                                                                                          >  8:149006/1‑101 (MQ=255)
                                                 gCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCTGGAAGCGGt                                                                                       >  5:237790/1‑136 (MQ=255)
                                                        ttATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATtgct                                                                                >  4:195579/1‑136 (MQ=255)
                                                             tgtgCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGctgctg                                                                            <  6:215327/135‑1 (MQ=255)
                                                                  tgcCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctg                                                                      >  5:241668/1‑136 (MQ=255)
                                                                  tgcCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctg                                                                      >  3:298175/1‑136 (MQ=255)
                                                                  tgcCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctg                                                                      >  8:252315/1‑136 (MQ=255)
                                                                  tgcCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctg                                                                      >  8:261100/1‑136 (MQ=255)
                                                                   gcCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     >  1:212684/1‑136 (MQ=255)
                                                                   gcCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     <  4:109319/136‑1 (MQ=255)
                                                                   gcCTGAGCAAAAGCTGGTGATCGGGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     <  6:161827/136‑1 (MQ=255)
                                                                   gcCTGAGCAAAAGCTGGTGATCGGGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGGGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     <  7:119489/136‑1 (MQ=255)
                                                                    ccTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctggc                                                                    <  1:19658/136‑1 (MQ=255)
                                                                    ccTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     >  1:80647/1‑135 (MQ=255)
                                                                    ccTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     >  7:23165/1‑135 (MQ=255)
                                                                    ccTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     <  7:261100/135‑1 (MQ=255)
                                                                    ccTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     <  1:233246/135‑1 (MQ=255)
                                                                    ccTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     >  2:283881/1‑135 (MQ=255)
                                                                    ccTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     >  2:65067/1‑135 (MQ=255)
                                                                    ccTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     <  4:293729/135‑1 (MQ=255)
                                                                    ccTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     <  5:51015/135‑1 (MQ=255)
                                                                    ccTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     >  3:166992/1‑135 (MQ=255)
                                                                    ccTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     >  4:90033/1‑135 (MQ=255)
                                                                    ccTGAGCAAAAGCTGGGGATCGGGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTgctggcgctgg                                                                     <  3:168448/135‑1 (MQ=255)
                                                                      tGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAg                                                                  >  8:224384/1‑136 (MQ=255)
                                                                       gAGCAAAAGCTGGTTATCCTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAACCGCGTGCTGGCGGAACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAgg                                                                 >  1:70937/1‑136 (MQ=255)
                                                                        atcAATAGCTGGTGATCGTGCGCTACGCCGATGACGGCGACAGCAGCTATCGACACGACGAATTGCTAAAGGGGGTGCTTGAGGTTCTGTCCGGGGAGGGGGCTTTAAGCGGTTATTGCTGCTGGCGCTGGCAggg                                                                <  7:270714/134‑1 (MQ=255)
                                                                        atcAAAAGCTGGTGATCTTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGGTATTGCTGCTGGCGCTGGCAggg                                                                <  3:81945/134‑1 (MQ=255)
                                                                        aGCAAACGGTGGTGGTCTGGCGCTACGCCGATGAGCGCGACAGCAGCTATCGACACGACGAATTGCTCTAGCGGGTGCTGGCGGTACTGGCGGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAggg                                                                <  3:202434/136‑1 (MQ=255)
                                                                        aGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAggg                                                                <  8:195211/136‑1 (MQ=255)
                                                                        aGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAggg                                                                <  7:271149/136‑1 (MQ=255)
                                                                        aGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAggg                                                                <  8:27150/136‑1 (MQ=255)
                                                                        aGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAggg                                                                <  4:140160/136‑1 (MQ=255)
                                                                         gtataaGCTGTTGATCGTGCGGTACCCCGATGACCGCGACAGCAGCTATCGAGACGACGTATTGCTCAAGCGGGTGGGGGCGGTACTGGCCGGGGAGGCGGCATGAAGCGGTAATTGCTGCTGGCGCTGGCAggg                                                                <  2:70937/131‑1 (MQ=255)
                                                                         gCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAggg                                                                <  1:7898/135‑1 (MQ=255)
                                                                          cAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCt                                                              >  4:54365/1‑136 (MQ=255)
                                                                               gCTGGTGAGCGTGCGCTACGCCGATTACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGt                                                         <  2:155391/136‑1 (MQ=255)
                                                                                     gATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTggg                                                   >  8:112308/1‑136 (MQ=255)
                                                                                             gcTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAAATGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAggg                                                                >  3:261379/1‑115 (MQ=255)
                                                                                             gcTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAAATGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAggg                                                                <  4:265729/115‑1 (MQ=255)
                                                                                             gcTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAAATGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAggg                                                                >  3:265729/1‑115 (MQ=255)
                                                                                             gcTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAAATGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAggg                                                                <  4:261379/115‑1 (MQ=255)
                                                                                                    cGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGcc                                    <  6:56104/136‑1 (MQ=255)
                                                                                                    cGATGACCGCGACAGCAGCTATCGACACGACGAATTGCGCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGcc                                    <  5:63279/136‑1 (MQ=255)
                                                                                                         aCCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGgcg                               >  5:217470/1‑136 (MQ=255)
                                                                                                         aCCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGgcg                               >  4:43202/1‑136 (MQ=255)
                                                                                                              gccagcagCTATCGACACGACGAATTGCTCACGCGGGTGCTGGCGGAACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCGTGTACTGGGCCTGGCTGGAACGCCAGGCGCTGGc                          >  3:132311/3‑136 (MQ=255)
                                                                                                              gACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGGCGCTGGc                          >  2:135283/1‑136 (MQ=255)
                                                                                                              gACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGGCGCTGGc                          >  3:278952/1‑136 (MQ=255)
                                                                                                               aCAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGGCGCTGGCg                         <  4:283644/136‑1 (MQ=255)
                                                                                                                cagcagCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGGCGCTGGCg                         >  2:254849/1‑135 (MQ=255)
                                                                                                                   cagcTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGGCGCTGGCGGACt                     <  3:99822/136‑1 (MQ=255)
                                                                                                                         tCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTAGTGGCCGGGGAGGGGGCATGAAGCGGTTTTTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGTGCCTGGCAGGAACGCCAGGCGCTGGCGGACTTCCCCg               <  4:75636/136‑1 (MQ=255)
                                                                                                                         tCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGGCGCTGGCGGACTTCCCCg               <  4:157487/136‑1 (MQ=255)
                                                                                                                         tCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGGCGCTGGCGGACTTCCCCg               <  8:89578/136‑1 (MQ=255)
                                                                                                                                   gaATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGGCGCTGGCGGACTTCCCCGGCATACtctc     >  5:22507/1‑136 (MQ=255)
                                                                                                                                   gaATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGGCGCTGGCGGACTTCCCCGGCATACtctc     <  1:110269/136‑1 (MQ=255)
                                                                                                                                      ttGCTCAAGCGGGGGCGGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGGCGCTGGCGGACTTCCCCGGCATACTCTCGGc  <  1:75774/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CTTGGCCCAGTGCACCGGCAGACACCTTCGCCGCCCTTGGCCACTGGGGGCAGGCTTTATATGTGCTGCCTGAGCAAAAGCTGGTGATCGTGCGCTACGCCGATGACCGCGACAGCAGCTATCGACACGACGAATTGCTCAAGCGGGTGCTGGCGGTACTGGCCGGGGAGGGGGCATGAAGCGGTTATTGCTGCTGGCGCTGGCAGGGCTCCTGTACTGGGCCTGGCAGGAACGCCAGGCGCTGGCGGACTTCCCCGGCATACTCTCGGC  >  ACB143/1025915‑1026184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: