Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 55,609 C→T 5.9% G76G (GGC→GGT alkJ_1 → Alcohol dehydrogenase [acceptor]

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB14355,6090CT 5.9% 233.8 / 3.3 71G76G (GGC→GGTalkJ_1Alcohol dehydrogenase [acceptor]
Reads supporting (aligned to +/- strand):  ref base C (33/32);  new base T (2/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-01

CTGCCGCGTGCTGCTGCTGGAGGCGGGTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAACGACGGCTGGGCGTGGAA  >  ACB143/55474‑55743
                                                                                                                                       |                                                                                                                                      
cTGCCGCGTGCTGGTGCTGGAGGCGGGTGGCCGTGACAATTATCGCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTcggc                                                                                                                                        <  5:252445/136‑1 (MQ=255)
cTGCCGCGTGCTGCTGCTGGAGGCGGGTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTcggc                                                                                                                                        <  2:259502/136‑1 (MQ=255)
   ccGCGTGCTGCTGCTGGAGGCGGGTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTcggcg                                                                                                                                       <  1:148663/134‑1 (MQ=255)
        tgctgctgcTGGAGGCGGGTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCGCTACTGCATCGGCAACCCGCGCACAGCCTGGTGCTTCAAGACCCACGCACAACCAGGCCTCGGGGGTcgc                                                                                                                                  >  4:245484/1‑134 (MQ=255)
           tgctgctgGAGGCGGGTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGCCTGGTGCTTCAAGCCCGCGGCACAACCTGGCCTCGGCGGTCGCGCttt                                                                                                                             >  2:77406/1‑136 (MQ=255)
           tgctgctgGAGGCGGGTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCttt                                                                                                                             >  7:15819/1‑136 (MQ=255)
             ctgctgGAGGCGGGTGGCCGTGACAATTATCCCTGGCTCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCttt                                                                                                                             <  1:288864/134‑1 (MQ=255)
             ctgctgGAGGCGGGTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCttt                                                                                                                             <  3:36993/134‑1 (MQ=255)
             ctgctgGAGGCGGGTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCttt                                                                                                                             <  3:26382/134‑1 (MQ=255)
             ctgctgGAGGCGGGTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCttt                                                                                                                             <  3:24768/134‑1 (MQ=255)
                   gAGGCGGGTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTa                                                                                                                       <  1:81683/134‑1 (MQ=255)
                        gggTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAgg                                                                                                                >  6:251601/1‑136 (MQ=255)
                        gggTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAgg                                                                                                                <  1:96763/136‑1 (MQ=255)
                         ggTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCTAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAggg                                                                                                               <  5:274192/136‑1 (MQ=255)
                         ggTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAggg                                                                                                               <  3:126765/136‑1 (MQ=255)
                         ggTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAggg                                                                                                               <  7:223680/136‑1 (MQ=255)
                          gTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAgggg                                                                                                              <  5:280184/136‑1 (MQ=255)
                           tGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGc                                                                                                             >  5:190970/1‑136 (MQ=255)
                            ggCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGGTTTGGGTTATCCAAGGGGc                                                                                                             >  3:164921/1‑135 (MQ=255)
                             gCCGTGACAATTATCCCTGGCTCCACATCCCCGTTGGTTACCTCAACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCGTTGGGGTATGCAAGGGGc                                                                                                             >  1:258810/1‑134 (MQ=255)
                             gCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGc                                                                                                             >  3:278336/1‑134 (MQ=255)
                                gTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAggg                                                                                                               <  7:30748/129‑1 (MQ=255)
                                gTGACAATTATCCATGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAggg                                                                                                               >  8:30748/1‑129 (MQ=255)
                                          taccTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCACGGCGGTCGCGCTTTGGGGTATACAATGGGCAAGGTGGTAGGTGGc                                                                                              >  8:4309/3‑136 (MQ=255)
                                             cTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGt                                                                                           <  1:143129/136‑1 (MQ=255)
                                               ggATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGGTATCCAAGGGGCAAGGTGCTAGGTGGCTGTtc                                                                                         >  4:272874/1‑136 (MQ=255)
                                                  tCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCGTTTGGTTATCAAAGGGGCAAGGTGCTAGGTGGCTGTtcgtc                                                                                      >  3:154429/1‑136 (MQ=255)
                                                  tCCAAATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTtcgtc                                                                                      <  7:216780/136‑1 (MQ=255)
                                                     acaTCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGAt                                                                                   <  3:42909/136‑1 (MQ=255)
                                                         ccccGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCCAc                                                                               >  6:62201/1‑136 (MQ=255)
                                                         ccccGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAAc                                                                               <  6:146733/136‑1 (MQ=255)
                                                          cccGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAAc                                                                               <  3:30172/135‑1 (MQ=255)
                                                          cccGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACg                                                                              <  7:179917/136‑1 (MQ=255)
                                                          cccGTTGGTTACCTCTACTGCATCGGCAACCAGCGCACAGACTGGTGCTTCAAGCCCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACg                                                                              <  3:92742/136‑1 (MQ=255)
                                                          cccGGTGGTTACCTCTACTGCATCGGCAACCCGCGCAGAGACTGGTGCTTCAAGACCGAGGCACACCCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACg                                                                              <  1:122963/136‑1 (MQ=255)
                                                            cGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGc                                                                            <  3:44832/136‑1 (MQ=255)
                                                              ttGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCAt                                                                          >  7:46904/1‑136 (MQ=255)
                                                                 gTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGAt                                                                       <  5:114334/136‑1 (MQ=255)
                                                                         tACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGc                                                               >  7:23416/1‑136 (MQ=255)
                                                                              cATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGTGGTCGCGCTTTGGGTTATCCAAggg                                                                                                               >  7:46297/1‑83 (MQ=255)
                                                                              cATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGTGGTCGCGCTTTGGGTTATCCAAggg                                                                                                               <  8:42148/83‑1 (MQ=255)
                                                                              cATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGTGGTCGCGCTTTGGGTTATCCAAggg                                                                                                               <  8:46297/83‑1 (MQ=255)
                                                                              cATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGTGGTCGCGCTTTGGGTTATCCAAggg                                                                                                               >  7:42148/1‑83 (MQ=255)
                                                                                 cGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGcc                                                         >  6:133486/1‑134 (MQ=255)
                                                                                  ggCAACCCGCGCACAGACTGGTGCTTCCAGACCGAGGCACAACCTGGCCTCGGAGGTCGCGCTTTGGGTCTTCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGGGGGGCCAAg                                                      <  4:23295/136‑1 (MQ=255)
                                                                                  ggCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAg                                                      <  3:177353/136‑1 (MQ=255)
                                                                                  ggCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAg                                                      <  1:190017/136‑1 (MQ=255)
                                                                                   gCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAgc                                                     >  7:132375/1‑136 (MQ=255)
                                                                                   gCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAgc                                                     >  5:134862/1‑136 (MQ=255)
                                                                                   gCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAgc                                                     >  4:61323/1‑136 (MQ=255)
                                                                                   gCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAgc                                                     >  3:82520/1‑136 (MQ=255)
                                                                                   gCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTAGGCGGTCGCGCTTTGGGTTATACAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAgc                                                     >  7:86083/1‑136 (MQ=255)
                                                                                      aCCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAgggg                                                                                                              >  8:122515/1‑76 (MQ=255)
                                                                                      aCCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAgggg                                                                                                              <  7:122515/76‑1 (MQ=255)
                                                                                          gcgcACTGACTGGTGCTTCAAGACCGAGGCACATCCTGGCCTCGGCGGTCGCGCTTTGGTTTAACCAAGGGGCAAGGTGCTAGGGGGCTGTTCGTCGATCAACGGCATCATCTCCATGCTGGGCCAAGCCGCCGAc                                              <  3:63940/136‑1 (MQ=255)
                                                                                                     ggTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTg                                   >  1:59315/1‑136 (MQ=255)
                                                                                                      gTGCTTCAAGACCGCGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCAtt                                    <  1:58334/134‑1 (MQ=255)
                                                                                                         cTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGcc                               >  8:71760/1‑136 (MQ=255)
                                                                                                         cTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGcc                               <  5:233462/136‑1 (MQ=255)
                                                                                                         cTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGcc                               >  7:279335/1‑136 (MQ=255)
                                                                                                             aaGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCgcgc                           <  6:109300/136‑1 (MQ=255)
                                                                                                               gACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCgcgc                           >  1:29909/1‑134 (MQ=255)
                                                                                                               gACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGGGCTAGGTGGCTGTTCGTCGCTCAACGGCATTATCTACCTGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCa                          >  6:27475/1‑135 (MQ=255)
                                                                                                                       cacaACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGCCCATTGGGCCGCGCAAGGCAacga                 >  4:192332/1‑136 (MQ=255)
                                                                                                                       cacaACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAacga                 <  3:195272/136‑1 (MQ=255)
                                                                                                                       cacaACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAacga                 >  8:186497/1‑136 (MQ=255)
                                                                                                                       cacaACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATAAACGGCATGAACTACATTCGGGGCCAAGCCGCCGACTACGACCATTGGGACACGCAATGCAacga                 >  4:60989/1‑136 (MQ=255)
                                                                                                                             cTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAACGACGGCTg           <  8:73045/136‑1 (MQ=255)
                                                                                                                             cTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAACGACGGCTg           >  6:227123/1‑136 (MQ=255)
                                                                                                                                  cTCGGCGGTCGCGCTTTGGGGTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAACGACGGCTGGGCGt      <  4:32349/136‑1 (MQ=255)
                                                                                                                                   tCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGCTCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAAAGACGGCTGGGCGTg     >  8:118388/1‑136 (MQ=255)
                                                                                                                                   tCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGCCCATTGGGCCGCGCAAGGCAACGACGGCTGGGCGTg     >  5:121901/1‑136 (MQ=255)
                                                                                                                                   tCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAacgac                >  5:278718/1‑125 (MQ=255)
                                                                                                                                   tCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAacgac                <  6:278718/125‑1 (MQ=255)
                                                                                                                                      gcggTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAACGACGGCTGGGCGTGGaa  <  8:60136/136‑1 (MQ=255)
                                                                                                                                      gcggTCGCGCTTGGGGTTATCCAAGGGTGAAGGTGCTAGGTGGCTGTTCGTGGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACCACCATTGGGCCGCGCAAGGCAACGACGGCTGGGCGTGGaa  <  2:275215/136‑1 (MQ=255)
                                                                                                                                       cggTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAACGACGGCTGGGCGTGGa   >  3:140586/1‑134 (MQ=255)
                                                                                                                                       cggTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCGGTTCGGCGCTCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAACGACGGCTGGGCGTGGa   >  7:139803/1‑134 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CTGCCGCGTGCTGCTGCTGGAGGCGGGTGGCCGTGACAATTATCCCTGGATCCACATCCCCGTTGGTTACCTCTACTGCATCGGCAACCCGCGCACAGACTGGTGCTTCAAGACCGAGGCACAACCTGGCCTCGGCGGTCGCGCTTTGGGTTATCCAAGGGGCAAGGTGCTAGGTGGCTGTTCGTCGATCAACGGCATGATCTACATGCGGGGCCAAGCCGCCGACTACGACCATTGGGCCGCGCAAGGCAACGACGGCTGGGCGTGGAA  >  ACB143/55474‑55743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: