Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	2,493,436	T→C	5.8%	F760S (TTC→TCC)	iutA →	Ferric aerobactin receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	2,493,436	0	T	C	5.8%	228.7 / 3.0	69	F760S (TTC→TCC)	iutA	Ferric aerobactin receptor
Reads supporting (aligned to +/- strand):  ref base T (16/48);  new base C (2/2);  total (18/51)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GCTGGAAGGGCGATGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACTACCAGGGGCGTGGGCGGACCTAT  >  ACB143/2493301‑2493566                                                                                                                                        |                                                                                                                                   gCTGGAAGGGCGATGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCtt                                                                                                                                    <  3:186337/136‑1 (MQ=255) gCTGGAAGGGCGATGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCtt                                                                                                                                    <  2:135649/136‑1 (MQ=255) gCTGGAAGGGCGATGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGACGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCtt                                                                                                                                    <  8:101694/136‑1 (MQ=255) acTGGAAGGGCGATGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCtt                                                                                                                                    <  2:28562/135‑1 (MQ=255)     gtaGGGCGATGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGt                                                                                                                                >  1:73801/3‑136 (MQ=255)     gAAGGGCGATGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGt                                                                                                                                >  1:101401/1‑136 (MQ=255)      aaGGGCGATGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTg                                                                                                                               <  4:253180/136‑1 (MQ=255)      aaGGGCGATGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTg                                                                                                                               <  3:25105/136‑1 (MQ=255)        gggCGATGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGcc                                                                                                                             <  2:53303/136‑1 (MQ=255)              tGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTCCAGTGCCGGTATc                                                                                                                       >  6:209440/1‑136 (MQ=255)              tGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTCCAGTGCCGGTATc                                                                                                                       >  6:213633/1‑136 (MQ=255)                gCCGCAGTGCACGCTTGCAGGCCAAGCATGGCTTCAGCCTGTAGGACGATTCCGATCTCGAGATTGACGGCTACACCACCTTCGACATGCTGGGCAGCGAGGACACCTGTTTCGGCACCTTCAGTGCCGGTATcc                                                                                                                      <  8:205794/135‑1 (MQ=255)                   gCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGaa                                                                                                                  <  8:280785/136‑1 (MQ=255)                         cACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGTGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACctgc                                                                                                             <  2:63451/135‑1 (MQ=255)                         cACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACctgc                                                                                                             <  2:84036/135‑1 (MQ=255)                         cACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACctgc                                                                                                             <  2:1238/135‑1 (MQ=255)                         cACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACctgc                                                                                                             <  8:47492/135‑1 (MQ=255)                          aCGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACctgctg                                                                                                           <  2:101401/136‑1 (MQ=255)                           cGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTgg                                                                                                          <  3:96454/136‑1 (MQ=255)                            gCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGGATCCAGAACCTGCTggc                                                                                                         >  7:267175/1‑135 (MQ=255)                             cTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGAc                                                                                                        <  4:124696/136‑1 (MQ=255)                             cTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGAc                                                                                                        <  2:57758/136‑1 (MQ=255)                             cTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGAc                                                                                                        <  8:183638/136‑1 (MQ=255)                             cTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGAc                                                                                                        <  1:83143/136‑1 (MQ=255)                             cTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGAc                                                                                                        <  4:189227/136‑1 (MQ=255)                             cTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGAc                                                                                                        <  8:243971/136‑1 (MQ=255)                             cTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGAc                                                                                                        <  5:289623/136‑1 (MQ=255)                             cTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGAAGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGAc                                                                                                        <  1:125377/136‑1 (MQ=255)                                  aGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCa                                                                                                   <  7:176545/136‑1 (MQ=255)                                    gCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGGCCCGTGAGTGGCGGGTTACAGAACCTGCGGGCCAAGCAGt                                                                                                 >  4:147274/1‑136 (MQ=255)                                    gCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCCCCTTCCGTGCCCGTTAACCGAAACAGCAGGACAACCAg                                                                                                  >  7:27327/1‑135 (MQ=255)                                    gCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCCGTGCCGGTTTCCAGAACCTGCGGGGCAAGCAGt                                                                                                 >  1:104374/1‑136 (MQ=255)                                    gCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCAACATCAGTGCCGGTATCCAGAACCCGCTGGTCAAGCat                                                                                                  >  1:18577/1‑134 (MQ=255)                                        aCCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAg                                                                                             <  5:39303/136‑1 (MQ=255)                                        aCCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAg                                                                                             <  8:149677/136‑1 (MQ=255)                                        aCCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAg                                                                                             <  1:103844/136‑1 (MQ=255)                                        aCCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAg                                                                                             <  2:73801/136‑1 (MQ=255)                                        aCCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAg                                                                                             <  1:85428/136‑1 (MQ=255)                                          cATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCa                                                                                           <  1:95365/136‑1 (MQ=255)                                           aTGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCCCCTTCAGTGCCGGTTTCCAGAACCTGCTGGACAAGCAGTTCAGCAc                                                                                          >  2:8163/1‑136 (MQ=255)                                            tGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACg                                                                                         <  2:131706/136‑1 (MQ=255)                                                   aGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATgg                                                                                   <  5:263330/135‑1 (MQ=255)                                                    gCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATgggg                                                                                 <  3:96423/136‑1 (MQ=255)                                                            gACGCTGCCGATCACGAGAGTGCCGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGGTTCGGCACCTGCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTg                                                                         <  6:70794/136‑1 (MQ=255)                                                             aggATGCCGATCCCGAGATTCACGCCTACACCACCTACGACCGGCTGGGCAGCCCGTACCCGGGTTTCGGCACC‑TCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCg                                                                       <  5:69810/134‑1 (MQ=255)                                                              cGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCg                                                                       <  2:66528/136‑1 (MQ=255)                                                              cGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCg                                                                       <  7:81710/136‑1 (MQ=255)                                                              cGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTGTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCg                                                                       <  2:182830/136‑1 (MQ=255)                                                                  gCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCCCCCTGACTGCCGGGGTCCAGCACCGGCTGGCAAACCACTTCAGCCCGGTTTGGGGGCAGCGTGGGGCgt                                                                   >  3:115820/1‑136 (MQ=255)                                                                  gCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCGGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACgt                                                                   >  3:140458/1‑136 (MQ=255)                                                                            agaTTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTc                                                         <  1:155288/136‑1 (MQ=255)                                                                            agaTTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTc                                                         <  3:84959/136‑1 (MQ=255)                                                                                         caccacCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACg                                              <  5:97802/134‑1 (MQ=255)                                                                                         caccacCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACg                                              <  6:13705/134‑1 (MQ=255)                                                                                             accTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCgg                                        <  6:281827/136‑1 (MQ=255)                                                                                               cTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCg                                      <  2:35452/136‑1 (MQ=255)                                                                                                    aCCTGCTGGGCAGCCAGGACACCGGTTTCGGCCCCTTGAGTTCCGGTTTGCAGAACCTGGGGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGGGACGTTGTTGGATTAGCCGACCTACGGGGCGGCGtttgt                                 >  7:123286/1‑132 (MQ=255)                                                                                                    aCCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTTTCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACCGCGTGCGACGGTGTTATATTCGCCGACCTACGGGCCGGCGGATCt                                 >  1:134571/1‑136 (MQ=255)                                                                                                    aCCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCt                                 >  3:86219/1‑136 (MQ=255)                                                                                                    aCCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCGTCAGTGCAGGTAACCAACACCTTCTGGACAAGCAGTAAAGCACGGTATGGGGACAGCGTGCGACGGTGTTCAATTAGCCGCCCAACGGGCCGGCCTAACt                                 >  8:6177/1‑136 (MQ=255)                                                                                                    aCCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCATCAGTGGCGGTTTACAGAACCAGGTGGCCAAGCAGTACAGCACCGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGCCCTACGGGCCGGCGTATCt                                 >  4:257292/1‑136 (MQ=255)                                                                                                            ggcaggcaGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACt                         <  7:138889/136‑1 (MQ=255)                                                                                                            ggCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTGGCCGACCTACGGGCCGGCGTATCTGTATGACt                         <  2:27836/136‑1 (MQ=255)                                                                                                                       cacCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACTACCAGGGGCGt              <  7:175067/136‑1 (MQ=255)                                                                                                                       cacCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACTACCAGGGGCGt              <  3:127756/136‑1 (MQ=255)                                                                                                                         ccGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACTACCAGGGGCGTgg            <  7:230610/136‑1 (MQ=255)                                                                                                                         ccGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACTACCAGGGGCGTgg            <  7:37563/136‑1 (MQ=255)                                                                                                                         ccGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACTACCAGGGGCGTgg            <  5:287258/136‑1 (MQ=255)                                                                                                                             tttCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACTACCAGGGGCGTGGGCgg        >  2:191408/1‑136 (MQ=255)                                                                                                                                   cACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACTACCAGGGGCGTGGGCGGACCtat  <  1:288923/136‑1 (MQ=255)                                                                                                                                   cACCTTCAGGGCCGGTATCCATAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGAGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACTACCAGGGGCGTGGGCGGACCtat  <  7:63773/136‑1 (MQ=255)                                                                                                                                   cACCTCCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACTACCAGGGGCGTGGGCGGACCtat  <  5:213633/136‑1 (MQ=255)                                                                                                                                   cACCTCCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACTACCAGGGGCGTGGGCGGACCtat  <  5:209440/136‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   GCTGGAAGGGCGATGGCCGCAGTGCACGCTTGCAGGCCAACCATGCCTTCAGCCTGAAGGACGATGCCGATCACGAGATTGACGGCTACACCACCTTCGACCTGCTGGGCAGCCAGGACACCGGTTTCGGCACCTTCAGTGCCGGTATCCAGAACCTGCTGGACAAGCAGTACAGCACGGTATGGGGACAGCGTGCGACGTTGTTCTATTCGCCGACCTACGGGCCGGCGTATCTGTATGACTACCAGGGGCGTGGGCGGACCTAT  >  ACB143/2493301‑2493566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑