Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 3,294,290 T→C 8.2% I52V (ATC→GTC)  CJ019_03098 ← Peptidyl‑prolyl cis‑trans isomerase cyp18

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,294,2900TC 8.2% 148.7 / 3.5 50I52V (ATC→GTC) CJ019_03098Peptidyl‑prolyl cis‑trans isomerase cyp18
Reads supporting (aligned to +/- strand):  ref base T (23/22);  new base C (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGGCTCCATGGTACGGGCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCTTGACGTACTGAACGAAGTTTTCGGTAGTCAGCGGCGCTTTCTCGGCGTCCAGTTGCAGGACGATGTCGCCGTGGT  >  ACB143/3294163‑3294418
                                                                                                                               |                                                                                                                                
tGCGGCTCCATGGTACGGGCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGATAATGCCGGCCTCGAAGCCGCCGCCATGGCTCATGAAGc                                                                                                                          >  6:87664/1‑136 (MQ=255)
tGCGGCTCCATGGTACGGGCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGGTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGc                                                                                                                          >  6:48311/1‑136 (MQ=255)
        cATTGTACTGGCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTTGATGCTGGCGCTGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATTAAGCCTTTGATc                                                                                                                  <  3:86187/136‑1 (MQ=255)
        cATGGTACGTGCCATGGCAATGCTGTACTTCTTGTTCTGCAGGCCGTTGTCGGCGTAGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGACGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGa                                                                                                                    <  8:239709/134‑1 (MQ=255)
        cATGGTACGGGCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATc                                                                                                                  <  1:157799/136‑1 (MQ=255)
        cATGGTACGGGCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATc                                                                                                                  <  1:86015/136‑1 (MQ=255)
        cATGGTACGGGCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATc                                                                                                                  <  4:115873/136‑1 (MQ=255)
        cATGGTACGGGCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATc                                                                                                                  <  8:78272/136‑1 (MQ=255)
        cATGGTACGGGCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATc                                                                                                                  <  3:174210/136‑1 (MQ=255)
         aTGGTACGGGCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGGTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATca                                                                                                                 <  7:263209/136‑1 (MQ=255)
                gggCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTg                                                                                                          <  2:227454/136‑1 (MQ=255)
                  gCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGa                                                                                                        <  2:228481/136‑1 (MQ=255)
                  gCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGa                                                                                                        <  6:32393/136‑1 (MQ=255)
                  gCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCAGGCAGCCTTTGATCACACGGTgg                                                                                                         >  2:235642/1‑135 (MQ=255)
                     aTGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCTGCTTCGTTCTGGATGGTGGCGCGGGTTTTCTTCTGGCTAATGCCGGCCTCGAAGCCGCCGCGCTGGTTCATGAAGCCTTTGATGACACTGTGGATTa                                                                                                     <  4:155935/136‑1 (MQ=255)
                     aTGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATa                                                                                                     <  5:225755/136‑1 (MQ=255)
                         cAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGt                                                                                                 >  8:229697/1‑136 (MQ=255)
                          aaTGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTg                                                                                                >  7:95763/1‑136 (MQ=255)
                          aaTGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTg                                                                                                >  5:237302/1‑136 (MQ=255)
                          aaTGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTg                                                                                                <  8:69228/136‑1 (MQ=255)
                          aaTGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTg                                                                                                <  2:164379/136‑1 (MQ=255)
                               tGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCAtt                                                                                           >  8:163209/1‑136 (MQ=255)
                               tGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCAtt                                                                                           >  4:282152/1‑136 (MQ=255)
                               tGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCAtt                                                                                           >  5:54725/1‑136 (MQ=255)
                               tGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCGTTGATCACACGGTGGAATACGGTGCCAtt                                                                                           >  6:86337/1‑136 (MQ=255)
                               tGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGGTTTATTATGGCTCATTCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACCGGGGGATAAGGTGCCaat                                                                                           >  6:225755/1‑134 (MQ=255)
                               tGTACTTCTGGATCTTCAGGCCGTTGTAGCCTTCGTTCTGGATGCTGGCGCGGGTTTTCATCTGGCTCATGCCGGCCTCCACGCCTGCGCCCTGGCTCATGAAGACTGTGATCACACGGTGGTATACGGTGCCAtt                                                                                           >  8:158987/1‑136 (MQ=255)
                                         gTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGc                                                                                   <  1:185786/134‑1 (MQ=255)
                                         gTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTATTCTGGCTCAATCCCGCCTCGAAGCCGCCGCCCTGGAACATGAAGCCTTTGATAACACGGTGGAATAAGGTGCCATTGTAGTGGccc                                                                                 >  2:185786/1‑135 (MQ=255)
                                              tcAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCtt                                                                            >  5:267016/1‑136 (MQ=255)
                                              tcAGGCCGTTGTCGGCTTAGTTCTGGATGATGGAGCGGGTTTTCATCTGGCTCATACCGGACTCGAAGCCGCCGCACAGGATCATGAAGCCGTAGAACAAACGGAGGAAAACCCTGCCATAGTACTTGccttcctt                                                                            >  2:209531/1‑136 (MQ=255)
                                                 ggCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCTTGAc                                                                         <  8:202469/136‑1 (MQ=255)
                                                          cGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCGCATTCCGGCCACGAAGCCGCCCCCCGGGATCATGAAGCCTTTTATAACACCGTGGAAAAACGTGCCATTGTAGTGGCCGTCCTTGACGGACTGAAc                                                                >  4:123039/1‑136 (MQ=255)
                                                          cGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTATGGATCAATCCCGCCTCGAAGCCGCCCCCCGGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCGTGACTTACTGTAc                                                                >  8:207836/1‑136 (MQ=255)
                                                               tCGTTCTGGATGCTGGCGCGGGTTTTATTCTGGCTCATGCCGGCCTCGAAGCCGCCCCCCTGGATCATTAACCCTTTTATCACACGGTGGAATACGGTGCCAATTTAGTGGCCGTCCTTGACGTACTGAACGAAGt                                                           >  8:43954/1‑136 (MQ=255)
                                                                cGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCTTGACGTACTGAACGAAGt                                                           <  2:126155/135‑1 (MQ=255)
                                                                 gTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATcac                                                                                                                >  8:95496/1‑81 (MQ=255)
                                                                 gTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATcac                                                                                                                <  7:95496/81‑1 (MQ=255)
                                                                                    gTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCTTGACGTACTGAACGAAGTTTTCGGTAGTCAGCGGCGCtt                                      <  6:96032/136‑1 (MQ=255)
                                                                                    gTTTTCTTCTGGCTCATGCCGGCCACGAAGCCCCCCCCCTGGCTCAAGAAGCCGTTGATAACACGGTGGAATAAGGTGCCATTGTAGTGGCCGGCCTTGACGTACTTAACGAAGTTTTAGGTAGTCACCGGCGCtt                                      >  8:196069/1‑136 (MQ=255)
                                                                                                   aTGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCTTGACGTACTGAACGAAGTTTTCGGTAGTCAGCGGCGCTTTCTCGGCGTCCAg                         <  4:143362/134‑1 (MQ=255)
                                                                                                       cGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCTTGACGTACTGAACGAAGTTTTCGGTAGTCAGCGGCGCTTTCTCGGCGTCCAGTTGCAg                   >  1:36182/1‑136 (MQ=255)
                                                                                                            tCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCTTGACGTACTGAACGAAGTTTTCGGTAGTCAGCGGCGCTTTCTCGGCGTCCAGTTGCAGGACGa              >  6:189318/1‑136 (MQ=255)
                                                                                                              gAAGCCGCCGCCCTGGACCATGAAGCCTTTGATCACACGGTGGAATACGGTg                                                                                                <  7:242499/52‑1 (MQ=255)
                                                                                                              gAAGCCGCCGCCCTGGACCATGAAGCCTTTGATCACACGGTGGAATACGGTg                                                                                                <  7:238681/52‑1 (MQ=255)
                                                                                                              gAAGCCGCCGCCCTGGACCATGAAGCCTTTGATCACACGGTGGAATACGGTg                                                                                                >  8:238681/1‑52 (MQ=255)
                                                                                                              gAAGCCGCCGCCCTGGACCATGAAGCCTTTGATCACACGGTGGAATACGGTg                                                                                                >  8:242499/1‑52 (MQ=255)
                                                                                                                     ccgccCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCTTGACGTACTGAACGAAGTTTTCGGTAGTCAGCGGCGCTTTCTCGGCGTCCAGTTGCAGGACGATGTCGCCGt     <  2:60303/136‑1 (MQ=255)
                                                                                                                        cccTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCTTGACGTACTGAACGAAGTTTTCGGTAGTCAGCGGCGCTTTCTCGGCGTCCAGTTGCAGGACGATGTCGCCGtggt  >  5:112634/1‑136 (MQ=255)
                                                                                                                        cccTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCTTGACGTACTGAACGAAGTTTTCGGTAGTCAGCGGCGCTTTCTCGGCGTCCAGTTGCAGGACGATGTCGCCGtggt  >  4:79874/1‑136 (MQ=255)
                                                                                                                               |                                                                                                                                
TGCGGCTCCATGGTACGGGCCATGGCAATGCTGTACTTCTTGTTCTTCAGGCCGTTGTCGGCTTCGTTCTGGATGCTGGCGCGGGTTTTCTTCTGGCTCATGCCGGCCTCGAAGCCGCCGCCCTGGATCATGAAGCCTTTGATCACACGGTGGAATACGGTGCCATTGTAGTGGCCGTCCTTGACGTACTGAACGAAGTTTTCGGTAGTCAGCGGCGCTTTCTCGGCGTCCAGTTGCAGGACGATGTCGCCGTGGT  >  ACB143/3294163‑3294418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑