Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,720,122 C→G 8.9% L49L (CTG→CTC)  sucD ← Succinate‑‑CoA ligase [ADP‑forming] subunitalpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,720,1220CG 8.9% 149.8 / 7.1 45L49L (CTG→CTC) sucDSuccinate‑‑CoA ligase [ADP‑forming] subunitalpha
Reads supporting (aligned to +/- strand):  ref base C (14/27);  new base G (2/2);  total (16/29)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

ATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTG  >  ACB143/4719991‑4720254
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aTCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                                                                                  >  1:251744/1‑136 (MQ=255)
aTCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                                                                                  <  5:143777/136‑1 (MQ=255)
 tCAGCTTTATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                                                                                  <  6:273942/135‑1 (MQ=255)
 tCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                                                                                  <  6:164356/135‑1 (MQ=255)
 tCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                                                                                  <  8:52981/135‑1 (MQ=255)
           gccgccAAAGGCAGCTTCCAGGATCGCGGCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCa                                                                                                                       <  4:182125/136‑1 (MQ=255)
                cAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCtt                                                                                                                  <  8:17823/136‑1 (MQ=255)
                  aaGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGc                                                                                                                >  4:242620/1‑136 (MQ=255)
                                  tCGAGTCTTTGCATAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACgccgcc                                                                                                <  3:230370/136‑1 (MQ=255)
                                        cTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCAt                                                                                          >  6:164755/1‑136 (MQ=255)
                                        cTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCAt                                                                                          >  3:2747/1‑136 (MQ=255)
                                         tttGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                                                                         <  7:258403/136‑1 (MQ=255)
                                           tGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                                                                         <  2:295912/134‑1 (MQ=255)
                                           tGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGGCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                                                                         <  6:30986/134‑1 (MQ=255)
                                             cAGAACGGTGCTGGTACGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCAtttta                                                                                      <  3:101678/135‑2 (MQ=255)
                                              aGAACGGCGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGt                                                                                    <  4:114565/136‑1 (MQ=255)
                                              aGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGt                                                                                    <  6:47756/136‑1 (MQ=255)
                                                   ggAGCTGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCg                                                                                >  3:134217/1‑135 (MQ=255)
                                                     aGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAg                                                                             <  3:39252/136‑1 (MQ=255)
                                                        cGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCCCCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGc                                                                           >  4:296917/1‑135 (MQ=255)
                                                        cGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGc                                                                           >  8:179720/1‑135 (MQ=255)
                                                          gTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTTAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAAt                                                                        <  7:177991/136‑1 (MQ=255)
                                                             cGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGc                                                                                  <  2:185538/123‑1 (MQ=255)
                                                             cGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGc                                                                                  >  1:185538/1‑123 (MQ=255)
                                                                 gATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGt                                                                 <  3:229175/136‑1 (MQ=255)
                                                                  aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGtt                                                                >  8:54166/1‑136 (MQ=255)
                                                                  aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGtt                                                                >  6:141488/1‑136 (MQ=255)
                                                                  aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCGTGGTGCCGTAGGCAATGGCCTGtt                                                                >  4:228764/1‑136 (MQ=255)
                                                                   tGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACATCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTc                                                               <  8:153150/136‑1 (MQ=255)
                                                                    gACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCCTCATGGTGCCGTAGGCAATGGCCTGTTCg                                                              >  5:283514/1‑136 (MQ=255)
                                                                       agAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGCCGTATGCAATGGCCTGTGCTGAg                                                           >  2:72674/2‑136 (MQ=255)
                                                                        gAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCTGAGt                                                          <  5:18499/136‑1 (MQ=255)
                                                                        gAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGt                                                          <  1:93704/136‑1 (MQ=255)
                                                                                      ggTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGc                                            <  2:158990/136‑1 (MQ=255)
                                                                                           cTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGcc                                       <  2:73520/136‑1 (MQ=255)
                                                                                                  ggCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAg                                <  2:251744/136‑1 (MQ=255)
                                                                                                            aCGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGa                      <  8:160206/136‑1 (MQ=255)
                                                                                                                     aaCACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTgg             >  7:96898/1‑136 (MQ=255)
                                                                                                                     aaCACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTgg             <  1:49332/136‑1 (MQ=255)
                                                                                                                      acacCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTgg             <  7:263291/135‑1 (MQ=255)
                                                                                                                          cGGCAGGCCCAGGTGGGTGTTGCCAACCTTGCCTGGGGTTACGCCGCCGAGCATCTTGGTGCCGTAGGCAATGGCCTGTGCGGAGTGGAAAGTACCCTGCGAGCCGGTGTAGCCCTGGCAGATGACTTTGGTATCt        <  3:73962/136‑1 (MQ=255)
                                                                                                                          cGGCAGGCCCAGGTGGGTGGTGCCACCGTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTTCGATCCGGTGAAGCCCTGGCAGATGACTTTGGTATCt        <  4:99575/136‑1 (MQ=255)
                                                                                                                             cAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTa     >  6:52647/1‑136 (MQ=255)
                                                                                                                               ggCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTAtt   >  2:22841/1‑136 (MQ=255)
                                                                                                                                gCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTg  <  4:296546/136‑1 (MQ=255)
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ATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTG  >  ACB143/4719991‑4720254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑