Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,720,161 G→A 7.8% V36V (GTC→GTT)  sucD ← Succinate‑‑CoA ligase [ADP‑forming] subunitalpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,720,1610GA 7.8% 166.3 / 2.4 51V36V (GTC→GTT) sucDSuccinate‑‑CoA ligase [ADP‑forming] subunitalpha
Reads supporting (aligned to +/- strand):  ref base G (18/29);  new base A (2/2);  total (20/31)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

CTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTACTTGCCCTCCGCAGCTTTGAC  >  ACB143/4720031‑4720291
                                                                                                                                  |                                                                                                                                  
cTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCAt                                                                                                                               >  3:2747/1‑136 (MQ=255)
cTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCAt                                                                                                                               >  6:164755/1‑136 (MQ=255)
 tttGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                                                                                                              <  7:258403/136‑1 (MQ=255)
   tGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                                                                                                              <  2:295912/134‑1 (MQ=255)
   tGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGGCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                                                                                                              <  6:30986/134‑1 (MQ=255)
     cAGAACGGTGCTGGTACGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCAtttta                                                                                                                           <  3:101678/135‑2 (MQ=255)
      aGAACGGCGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGt                                                                                                                         <  4:114565/136‑1 (MQ=255)
      aGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGt                                                                                                                         <  6:47756/136‑1 (MQ=255)
           ggAGCTGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCg                                                                                                                     >  3:134217/1‑135 (MQ=255)
             aGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAg                                                                                                                  <  3:39252/136‑1 (MQ=255)
                cGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCCCCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGc                                                                                                                >  4:296917/1‑135 (MQ=255)
                cGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGc                                                                                                                >  8:179720/1‑135 (MQ=255)
                  gTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTTAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAAt                                                                                                             <  7:177991/136‑1 (MQ=255)
                     cGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGc                                                                                                                       >  1:185538/1‑123 (MQ=255)
                     cGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGc                                                                                                                       <  2:185538/123‑1 (MQ=255)
                         gATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGt                                                                                                      <  3:229175/136‑1 (MQ=255)
                          aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGtt                                                                                                     >  6:141488/1‑136 (MQ=255)
                          aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGtt                                                                                                     >  8:54166/1‑136 (MQ=255)
                          aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCGTGGTGCCGTAGGCAATGGCCTGtt                                                                                                     >  4:228764/1‑136 (MQ=255)
                           tGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACATCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTc                                                                                                    <  8:153150/136‑1 (MQ=255)
                            gACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCCTCATGGTGCCGTAGGCAATGGCCTGTTCg                                                                                                   >  5:283514/1‑136 (MQ=255)
                               agAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGCCGTATGCAATGGCCTGTGCTGAg                                                                                                >  2:72674/2‑136 (MQ=255)
                                gAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCTGAGt                                                                                               <  5:18499/136‑1 (MQ=255)
                                gAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGt                                                                                               <  1:93704/136‑1 (MQ=255)
                                              ggTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGc                                                                                 <  2:158990/136‑1 (MQ=255)
                                                   cTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGcc                                                                            <  2:73520/136‑1 (MQ=255)
                                                          ggCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAg                                                                     <  2:251744/136‑1 (MQ=255)
                                                                    aCGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGa                                                           <  8:160206/136‑1 (MQ=255)
                                                                             aaCACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTgg                                                  >  7:96898/1‑136 (MQ=255)
                                                                             aaCACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTgg                                                  <  1:49332/136‑1 (MQ=255)
                                                                              acacCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTgg                                                  <  7:263291/135‑1 (MQ=255)
                                                                                  cGGCAGGCCCAGGTGGGTGTTGCCAACCTTGCCTGGGGTTACGCCGCCGAGCATCTTGGTGCCGTAGGCAATGGCCTGTGCGGAGTGGAAAGTACCCTGCGAGCCGGTGTAGCCCTGGCAGATGACTTTGGTATCt                                             <  3:73962/136‑1 (MQ=255)
                                                                                  cGGCAGGCCCAGGTGGGTGGTGCCACCGTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTTCGATCCGGTGAAGCCCTGGCAGATGACTTTGGTATCt                                             <  4:99575/136‑1 (MQ=255)
                                                                                     cAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTa                                          >  6:52647/1‑136 (MQ=255)
                                                                                       ggCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTAtt                                        >  2:22841/1‑136 (MQ=255)
                                                                                        gCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTg                                       <  4:296546/136‑1 (MQ=255)
                                                                                            aGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCa                                   <  5:99889/136‑1 (MQ=255)
                                                                                            aGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCa                                   <  3:144274/136‑1 (MQ=255)
                                                                                              gtgggtggTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAgg                                 <  6:134605/136‑1 (MQ=255)
                                                                                                      tgCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCAt                         >  3:113130/1‑136 (MQ=255)
                                                                                                      tgCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCAt                         >  3:272930/1‑136 (MQ=255)
                                                                                                      tgCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCAt                         >  5:220081/1‑136 (MQ=255)
                                                                                                      tgCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCAt                         >  1:223969/1‑136 (MQ=255)
                                                                                                         cACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTAc                      <  7:143535/136‑1 (MQ=255)
                                                                                                                gCCTGGGGTTACGC‑‑CCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTACTTGCCCt               <  2:98824/134‑1 (MQ=255)
                                                                                                                 ccTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTACTTGCCCt               <  8:240786/135‑1 (MQ=255)
                                                                                                                   tGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTACTTGCCCTCCg            >  7:18734/1‑136 (MQ=255)
                                                                                                                        ttACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTACTTGCCCTCCGCAGCt       >  4:44710/1‑136 (MQ=255)
                                                                                                                           cgccgcCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAATTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTACTTGCCCTCCGCAGCTTTg    <  3:242620/136‑1 (MQ=255)
                                                                                                                           cgccgcCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTACTTGCCCTCCGCAGCTTTg    <  4:129869/136‑1 (MQ=255)
                                                                                                                           cgccgcCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTACTTGCCCTCCGCAGCTTTg    <  1:160651/136‑1 (MQ=255)
                                                                                                                              cgccgACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTACTTGCCCTCCGCAGCTTTGAc  >  3:236857/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                                  
CTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTACTTGCCCTCCGCAGCTTTGAC  >  ACB143/4720031‑4720291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑