Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,481,135 A→G 8.9% D155G (GAC→GGC)  purH → Bifunctional purine biosynthesis protein PurH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,481,1350AG 8.9% 134.0 / 4.2 45D155G (GAC→GGC) purHBifunctional purine biosynthesis protein PurH
Reads supporting (aligned to +/- strand):  ref base A (16/25);  new base G (2/2);  total (18/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

GAAGCCACCATTTCCAAGCCTGGCTGTGACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCGCCAACTACATGGGCACCATCGACCA  >  ACB143/5481005‑5481270
                                                                                                                                  |                                                                                                                                       
gAAGCCACCATTTCCAAGCCTGGCTGTGACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGCCCATGGTCCGTTCGGCCGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACg                                                                                                                                    >  5:26266/1‑136 (MQ=255)
gAAGCCACCATTTCCAAGCCTGGCTGTGACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACg                                                                                                                                    >  2:200453/1‑136 (MQ=255)
gAAGCCACCATTACCAAGCCAGGCTGTGACCTGCCCACAGCCATCGAGAACATCGACATCGGCGGCCCGACCAATGTCCGTTCGCCACCCAAGAACAACAAGGACGTGGCAATCGTGGTCAACGCCAGAGACTACg                                                                                                                                    >  7:123250/1‑136 (MQ=255)
 aaGCCACCATTTCCAAGCATGGCTGTGACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGc                                                                                                                                   >  1:12996/1‑136 (MQ=255)
    ccaccaTTTCCAAGCCTGGCTGTGACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGGCTACGCCgg                                                                                                                                >  1:101011/1‑136 (MQ=255)
    ccaccaTTTCCAAGCCTGGCTGTGACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGGCTACGCCgg                                                                                                                                >  1:96840/1‑136 (MQ=255)
            tCCAAGCCTGGCTGTGACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCAtcgtc                                                                                                                         <  1:235022/135‑1 (MQ=255)
               aaGCCTGGCTGTGACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAg                                                                                                                     >  3:162236/1‑136 (MQ=255)
                        tgtgACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAgg                                                                                                            >  5:83092/1‑136 (MQ=255)
                          tgACCTGCCGACCGCCATCGCGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAgg                                                                                                            <  4:263656/134‑1 (MQ=255)
                          tgACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAgg                                                                                                            <  6:267167/134‑1 (MQ=255)
                          tgACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAgg                                                                                                            <  3:213561/134‑1 (MQ=255)
                          tgACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAgg                                                                                                            <  7:47109/134‑1 (MQ=255)
                          tgACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAgg                                                                                                            <  4:289019/134‑1 (MQ=255)
                             ccTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGt                                                                                                       <  1:50029/136‑1 (MQ=255)
                             ccTGCCGACCGCCATCGAGAACATCGACATCGACGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGt                                                                                                       <  7:38581/136‑1 (MQ=255)
                               tGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGCACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTgg                                                                                                     >  5:162914/1‑136 (MQ=255)
                                 ccGACCGCCATCGAGAACATCGACATCGGCGGCCCGCCCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTgg                                                                                                     <  8:267049/134‑1 (MQ=255)
                                 ccGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTgg                                                                                                     <  5:228100/134‑1 (MQ=255)
                                    aCCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGa                                                                                                <  2:85087/136‑1 (MQ=255)
                                                 acatcgacatcgGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAgcg                                                                                   <  5:251049/136‑1 (MQ=255)
                                                   atcgacatcgGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCt                                                                                 <  4:115648/136‑1 (MQ=255)
                                                     cgacatcgGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTc                                                                               >  7:176896/1‑136 (MQ=255)
                                                      gacatcgGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTc                                                                               >  1:31687/1‑135 (MQ=255)
                                                        catcgGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGAc                                                                            <  1:95133/136‑1 (MQ=255)
                                                          tcgGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCt                                                                          >  4:4613/1‑136 (MQ=255)
                                                               ggcCCGACCATGGTCCGTTCGTCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGc                                                                     <  6:237812/136‑1 (MQ=255)
                                                                gcCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGCCCTGATGCt                                                                    >  6:175946/1‑136 (MQ=255)
                                                                  ccGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCa                                                                  <  6:83092/136‑1 (MQ=255)
                                                                           gTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTc                                                          <  4:129526/135‑1 (MQ=255)
                                                                              cGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCAGCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGc                                                      <  7:81346/136‑1 (MQ=255)
                                                                                  cGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGcaca                                                   <  7:91511/135‑1 (MQ=255)
                                                                                  cGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGcaca                                                   <  7:91517/135‑1 (MQ=255)
                                                                                   ggCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACt                                                 >  4:262102/1‑136 (MQ=255)
                                                                                   ggCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACt                                                 >  1:14456/1‑136 (MQ=255)
                                                                                   ggCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACt                                                 >  1:115080/1‑136 (MQ=255)
                                                                                                cacaAGGACTTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTacgacg                                    <  2:288328/136‑1 (MQ=255)
                                                                                                        cGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATc                            <  1:200453/136‑1 (MQ=255)
                                                                                                         gTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCg                           <  3:4613/136‑1 (MQ=255)
                                                                                                             ccATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCGCCaa                       >  5:93071/1‑136 (MQ=255)
                                                                                                                    ggTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCGCCAACTACATg                >  7:198385/1‑136 (MQ=255)
                                                                                                                              aGCGGCTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCGCCAACTACATGGGCACCATCg      <  2:96840/136‑1 (MQ=255)
                                                                                                                              aGCGGCTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCGCCAACTACATGGGCACCATCg      <  2:101011/136‑1 (MQ=255)
                                                                                                                              aGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCGCCAACTACATGGGCACCATCg      <  2:41761/136‑1 (MQ=255)
                                                                                                                              aGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCGCCAACTACATGGGCACCATCg      <  1:85681/136‑1 (MQ=255)
                                                                                                                                  aCTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCGCCAACTACATGGGCACCATCGAc    >  1:150155/1‑134 (MQ=255)
                                                                                                                                  aCTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCGCCAACTACATGGGCACCATCGACCa  <  6:132292/136‑1 (MQ=255)
                                                                                                                                  aCTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCGCCAACTACATGGGCACCATCGACCa  <  5:43588/136‑1 (MQ=255)
                                                                                                                                  aCTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCGCCAACTACATGGGCACCATCGACCa  <  2:170851/136‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                       
GAAGCCACCATTTCCAAGCCTGGCTGTGACCTGCCGACCGCCATCGAGAACATCGACATCGGCGGCCCGACCATGGTCCGTTCGGCAGCCAAGAACCACAAGGACGTGGCCATCGTGGTCAACGCCAGCGACTACGCCGGCATCGTCGAAGGCCTCAAGGCCGGTGGCCTGACCTATGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCACACTGCCGCCTACGACGGCATGATCGCCAACTACATGGGCACCATCGACCA  >  ACB143/5481005‑5481270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑