Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 6,129,757 A→T 31.0% H430Q (CAT→CAA)  copA_4 ← Copper resistance protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1436,129,7570AT31.0% 51.9 / 4.7 42H430Q (CAT→CAA) copA_4Copper resistance protein A
Reads supporting (aligned to +/- strand):  ref base A (9/19);  new base T (3/8);  total (14/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCG  >  ACB143/6129623‑6129889
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ccAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCAt                                                                                                                                     <  1:293311/136‑1 (MQ=255)
      aTCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCAGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTcc                                                                                                                                >  2:9440/1‑135 (MQ=255)
           gCTTTGGTCCTTTTCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTTCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCAt                                                                                                                              <  1:112917/132‑1 (MQ=255)
           gCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCAGCCGTGGTCCATCACAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCTTGGTCCATGCCa                                                                                                                          >  1:44877/1‑136 (MQ=255)
            cTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGTCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAg                                                                                                                         <  1:74686/136‑1 (MQ=255)
               tGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGcc                                                                                                                       <  7:57104/135‑1 (MQ=255)
                    cATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTg                                                                                                                 <  1:125634/136‑1 (MQ=255)
                       atCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGGAGCAGTGGTCCATCTCAGACATTTTGATGTGCTCCATGCCGGACATGCTGCCGTGGGACATGCCAACCAGTGTGcag                                                                                                              >  6:79677/1‑134 (MQ=14)
                        tCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCtgt                                                                                                             >  2:556/1‑136 (MQ=255)
                              aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCa                                                                                                       <  8:186631/136‑1 (MQ=255)
                              aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCAGCCGTGGTCCATCACAGCCATTTTGCTGTGCTCCATGCCGGCCATGCTGCGATGGTCCAGACCCGCCAGGTTGCTTTGGTCCa                                                                                                       >  5:19708/1‑136 (MQ=17)
                                   gccTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCAGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGATGCCTTGGTCCATACCCGCCTTTTTGCTGTGGTCCATGCCg                                                                                                  >  4:266917/1‑136 (MQ=17)
                                        ggTTCATCCCAGCCATCGTGCAGTGGTCCATGCCGGCCATTCCGCCGTGGTCCATCTCAGCCATTTTGCTGAGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTc                                                                                               <  6:217653/134‑1 (MQ=17)
                                        ggTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCa                                                                                              <  3:29326/135‑1 (MQ=255)
                                          tCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGt                                                                                           <  1:284612/136‑1 (MQ=255)
                                            catcccatccATCTTGCTGTGGTCCATTCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTg                                                                                         <  7:199500/136‑1 (MQ=255)
                                            catcccatccATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTg                                                                                         <  4:239247/136‑1 (MQ=255)
                                            cATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGtt                                                                                          <  7:45893/135‑1 (MQ=255)
                                                 cAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGtt                                                                                          >  5:157420/1‑130 (MQ=255)
                                                 cAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGtt                                                                                          <  6:157420/130‑1 (MQ=255)
                                                                    cATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGTTCCATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTg                                                                 <  4:127619/136‑1 (MQ=255)
                                                                    cATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGGTCCATGCCGGCCATGATGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCTTGTTTCCGTGGTCCATCCCAGCCATGTTg                                                                 >  7:73235/1‑136 (MQ=14)
                                                                    cATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGATGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCAATTTTg                                                                 >  7:165744/1‑136 (MQ=255)
                                                                    cATGCCCGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGGTCCATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGACGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTg                                                                 <  6:84400/136‑1 (MQ=255)
                                                                                 cggccgTGGTCCATCGCAGCCATTTTGCTGGGCTCCATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGc                                                    <  7:91528/134‑1 (MQ=14)
                                                                                   gccgTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCCTGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGACGTGGTCGAGCCCAGCCATATTGCAGTGGTCCATGCCg                                                  >  8:63037/1‑136 (MQ=17)
                                                                                    ccgTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCTTGGTCCATCCCAGCCAGTTTGCTGTGCTCCATGCCg                                                  >  1:73534/1‑135 (MQ=255)
                                                                                      gTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGcc                                               <  6:101210/136‑1 (MQ=255)
                                                                                           ccATCTCTTCCATTATGCTTTGATCCATGACGTCCATGATGCCTTGGTCCATCCCATCCATTTTGCTGTGGTCCATGCCGGCCATGATGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGtt                                          <  1:219546/136‑1 (MQ=11)
                                                                                                  aGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGt                                     <  3:138462/134‑1 (MQ=255)
                                                                                                  aGCCATTTTGCTGTGATCCATCCCGGCCATGCTGCCATGGTCCATGCCAGACATTTTGCTGTGGTCCATTCCTGGCATTTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGt                                     <  7:42122/134‑1 (MQ=14)
                                                                                                    cgaTTTTCCTGTGCTCCATGCCGGACATGCTGCCTTGGACCATCCCTTCCATTTTGCTGTGGTCCATGCCGGTCATGCTGCCGTGGTCCATCCCAGCCATGTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTc                                 <  8:177744/134‑1 (MQ=11)
                                                                                                               tgttCCATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGc                        <  7:95142/131‑1 (MQ=255)
                                                                                                               tgATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGcc                       <  1:160352/135‑1 (MQ=255)
                                                                                                                   cgaggCCCGCCATCCGGCCGTCGTCCATCCCAGCCATTTTGCTGGGGTCCATGCCGGCCATGATGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATCCCGGCCATGCTGCCGTGGTCCATCCCAGCCAtttt                  <  8:189048/132‑1 (MQ=11)
                                                                                                                   ccATGCCGGCCATGCCGCCTTGGCCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGATGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt                  <  2:148680/136‑1 (MQ=255)
                                                                                                                    catcgcGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt                  <  3:282511/130‑1 (MQ=255)
                                                                                                                        ccGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCtgt             <  5:94921/136‑1 (MQ=255)
                                                                                                                        ccGGCCATGCGGCCATGGTCCATCCCATCCATTTTGCTGTGGTCCATGACGGCCATGACGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCtgt             <  8:18479/136‑1 (MQ=14)
                                                                                                                               tgctgcCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATTCGGGGCATGTTGCCGTGGTCCATACCAGCCATTTTGCAGTGGACCAAGCCGCACATGTTGCGGTCGTCCATACCGGCCATTTTGCtgtgtgcaa       >  8:122695/1‑130 (MQ=14)
                                                                                                                               tgctgcCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATACCGGCCATTTTGCTGTGGTcaa       >  4:244197/1‑133 (MQ=255)
                                                                                                                                   gcCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCg  >  4:275598/1‑136 (MQ=255)
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CCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCG  >  ACB143/6129623‑6129889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑