Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 627,234 A→C 27.4% S164R (AGC→CGC)  acoR_1 → Acetoin catabolism regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143627,2340AC27.4% 26.9 / 3.5 41S164R (AGC→CGC) acoR_1Acetoin catabolism regulatory protein
Reads supporting (aligned to +/- strand):  ref base A (11/16);  new base C (10/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.80e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

TGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAGATGATCGAAAGCT  >  ACB143/627099‑627366
                                                                                                                                       |                                                                                                                                    
tgGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGGCGCGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCGGACCTGCTCGGCCa                                                                                                                                      <  2:20443/136‑1 (MQ=255)
   cTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCTGGCCGGCTCGGCCAccc                                                                                                                                   >  1:153002/1‑136 (MQ=255)
         gcTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCTGTCCGGCTCGGGCCGCCCGgggt                                                                                                                             >  7:25912/1‑136 (MQ=255)
         gcTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGAGCTGACCCTCCATCAGCACGAGCCCTTCCGTGGCCGTCCCCACCGGCGGACCGACGCGGCACGCCCgggtg                                                                                                                             >  8:199770/1‑133 (MQ=255)
                         cgcgcgAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCTGGCCGGCGCGGGCCGCCCCGGGTTGGGCCCGCATggggc                                                                                                             >  3:122572/1‑133 (MQ=255)
                          gcgcgAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTGGCACCGGGCGGGGCTGGGCGGGCCGGCCGGGGTTGGACCCGCATgggggc                                                                                                            >  3:110230/1‑132 (MQ=255)
                          gcgcgAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTGACCCCCGGCTGACCAGCGAGGGCCGCCCGGTGTTGGGCCCCCATgggggc                                                                                                            >  8:249302/1‑132 (MQ=255)
                          gcgcgAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCAGCTCGGCCAGCCCGGGGTTCGACCCGCATTGCgcc                                                                                                            >  6:243876/1‑134 (MQ=255)
                              gcgggCACAAATGCCAGCGCAACATGTCTGGCCGAGCGCCAGGCGCTGACCATCCATCACACCCAGCACTTCGGTGGCCGGCACACCGGGCTGACCTGCTCGGCCAGCCGGTTGTTCGACCCGCATGGCGACctgc                                                                                                        <  2:5824/134‑1 (MQ=255)
                              gAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCAAACCCGGCAGACCTGCGCGGCCAGCCCGGTGTTAGACCCGCCTGGCCACcagc                                                                                                        >  6:266033/1‑136 (MQ=255)
                               agaccgccAATGCCAGCGGCACTTGGCTGGGCGAGCGCCCGGCGCTTACCATCCATCACACCGCGCACTTCCGGGGCCGCCACCCCGGGCTGACCGGCGCGGCCAGCCCGGTGTTCGACCCGCATGGCGACctgct                                                                                                       <  6:216239/130‑1 (MQ=255)
                               aGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCTGGCCTGGGCGGCCCGCCCGGTGTTCGTCCCGCATGGCGACcggcg                                                                                                       >  7:59509/1‑135 (MQ=255)
                                cagCACCAATGGCTTCGGCAAATGTCTGGGCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGGAACACGGGGCTGACCTGCGCGGCCAGCCCGGTGTTCGACCCGCATGGCGACctgc                                                                                                        <  2:22324/132‑1 (MQ=255)
                                 ggCACAAATGGCAGCGCCACTTGTCTGGCCGAGCGCCAGGCGCTGACCATCCATCAGACCGACCCCTCCCGGGGCCGCCACACCGGGCTGACCTGCGCGGCCAGCCGGGTGTTCGACCCGCATGGCGACCTGCTgg                                                                                                     <  2:278839/136‑1 (MQ=255)
                                    cccAATGGCAGCGGCACAGGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGACCGAGCCATTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCgg                                                                                                  <  6:286547/135‑1 (MQ=255)
                                    acaaaTGGCATCGGCACAGGTGGGGGCGAGCGCCAGGCGGTGACAATCCTTCAGAACGAGCACTCCCGGGGCCGGCACCCCGGGCTGACGTGCTCGGCCAGCCGGGTGTTCGACCCGCATGGCGACCTGCTGGCgg                                                                                                  <  4:25796/133‑1 (MQ=255)
                                    acaaaTGGCATCGCAACTGGCCGGGGCGAGCGCAAGGCGCTAACAAACCAAAACACCCCCCACTTCCGTGGCCGCCACACGGGGCTGACCTGCGCGCCCCGCCGGGTCTTCGACCCGCATGGCGACCTGCTGGCgg                                                                                                  <  6:170124/133‑1 (MQ=255)
                                    aCCAATGGCAGCGCCACATGTCTGGGCGCGCGCCAGGCGCTGACCATCCATCAGACCGACCACTTCCGGGGCCGTCACACCGGGCTGACCTGCGCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCgg                                                                                                  <  2:261686/136‑1 (MQ=255)
                                         tGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGTCCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGGCCTGCTGGCGGTGCTc                                                                                             >  7:28897/1‑136 (MQ=255)
                                               cGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCGGACCTGCTCGGCCAGCCCGGTGTTCGCCCCGCATGGCGCCCAGCTGGCGGTGCTCGACgtg                                                                                       >  2:161269/1‑136 (MQ=255)
                                                ggCACATGGCTGGTCGAGCGCCCGGCGCTGACCAGCCCTCCCACCGAGCACTACCGGGGCCGTCACACCGGGCTGACCTGCTGGGCCCGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACgtgt                                                                                      <  4:90256/136‑1 (MQ=255)
                                                   acaTGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCCCACCCGGCGGCCCGGCTCGGCCCGCCCCGGGTTGGACCCGCAAGGCGCCCGGCTGGCGGTGCTCGCCgcggggt                                                                                   >  5:216239/1‑130 (MQ=255)
                                                   acaTGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCTGACCTGCTCGGGCAGCCCGGTGTTGGACCCGCCGGGCGACCTGCTGGCGGTGCGCGACGTGGccg                                                                                   >  4:252732/1‑135 (MQ=255)
                                                     aTGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCctct                                                                                 <  7:137877/136‑1 (MQ=255)
                                                               gAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCCCACCCGGGTGGCCGGGGCGGGCCGCCCGGGGTTCGCCCCGCCTGGCGCCCTGCTGGCGGTGGGGGCCGGGGCGGCTGCGCGCCCCg                                                                       >  4:187158/1‑136 (MQ=255)
                                                                   gcaaGGCGCTGACCATCCATCACAACGAGCACTGCCGTGGCCGTCACACCGGGCTGACCTGCGCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGt                                                                   <  1:195985/133‑1 (MQ=255)
                                                                    ccAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTGACACCCGGCGGCCCTGCTCGGCCCGCCCCGGGGTCGGCCCCCCTGGCGCCCGGCGGGCGGTGGTCGACGTGTCCTCTGCGCGcccccgcgtc                                                                  >  5:212081/1‑130 (MQ=255)
                                                                        gggcTGACCATCCATCAGAAGGAGCACTTCCGGGGCCGTCACACCGGGCTGACCTGCGCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATcgc                                                              <  1:161269/134‑1 (MQ=255)
                                                                        gcgcTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACCCCGGGCTGACCTGCTCGGCCAGCCGGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATcgc                                                              <  4:25686/136‑1 (MQ=255)
                                                                         cgcTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCTGACCTGCTCGGCCAGCCCGGGGGTCGACCCCCATGGCGCCCTGCTGGCGGTGCTCGACGGGTCCTCGGCGCGCCCCGACGGATcgcg                                                             >  1:212278/1‑136 (MQ=255)
                                                                              aCCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCaa                                                         <  2:171646/135‑1 (MQ=255)
                                                                                     atcAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTTTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGtt                                                 <  2:13817/136‑1 (MQ=255)
                                                                                         gaccGAGCACTTCCGGGGCCGTCACACGGGGGTGACCTGCTCGGCCAGCCGGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCcac                                             <  7:131857/133‑1 (MQ=255)
                                                                                           aCGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCCGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTTTCGCGGCAAAGACAGTTCCacac                                           >  6:207305/1‑136 (MQ=255)
                                                                                                cACTTCCGTGGCCGTCACAACGCGCTGACCTGCTCGGCCCGCCCCGGGTTCGACCCCCATGGCGCCCGGCTGGCGGGGCTCGACGTGTCCACAGCGCGCCCCCACGGATCGCGGCAAAGCCCGTTCCACACCCTgg                                      >  8:77871/1‑136 (MQ=255)
                                                                                                                     ggggggACCGGCTCGGCCTGCCGGTTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGa                 <  4:110230/131‑1 (MQ=255)
                                                                                                                         tGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAgat             >  4:33064/1‑136 (MQ=255)
                                                                                                                             cTGGTCGGCCAGCGGGTTGATCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCTTTGGTCAACCTGTCGGCGAAGATGATc         <  8:172868/136‑1 (MQ=255)
                                                                                                                             cTGCTCGGCCTGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAGATGATc         <  1:31421/136‑1 (MQ=255)
                                                                                                                                   ggCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAGATGATCGAAAgc   >  2:292192/1‑136 (MQ=255)
                                                                                                                                   ggCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAGATGATCGAAAgc   >  1:63990/1‑136 (MQ=255)
                                                                                                                                    gCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAGATGATCGAAAgct  <  4:45266/136‑1 (MQ=255)
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TGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAGATGATCGAAAGCT  >  ACB143/627099‑627366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑