Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 843,700 A→T 7.6% intergenic (‑233/‑572) CJ019_00805 ← / → hemA hypothetical protein/Glutamyl‑tRNA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143843,7000AT 7.6% 190.5 / 4.1 61intergenic (‑233/‑572)CJ019_00805/hemAhypothetical protein/Glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base A (22/34);  new base T (2/3);  total (24/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGAAATGGACTACAAGACTGTGGATAAATCTTTTGGA  >  ACB143/843567‑843832
                                                                                                                                     |                                                                                                                                    
cttcttGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAAt                                                                                                                                    <  2:113417/136‑1 (MQ=255)
         ggTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCg                                                                                                                            <  1:42008/135‑1 (MQ=255)
          gtgtgtCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCTCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTa                                                                                                                          <  7:222700/136‑1 (MQ=255)
          gtgtgtCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTa                                                                                                                          <  8:273506/136‑1 (MQ=255)
          gtgtgtCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTa                                                                                                                          <  3:208965/136‑1 (MQ=255)
              gtCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGc                                                                                                                      <  8:124945/136‑1 (MQ=255)
                  ccAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGtc                                                                                                                  <  8:87392/136‑1 (MQ=255)
                       taagtGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGt                                                                                                             <  3:287811/136‑1 (MQ=255)
                              gCATCATTCCAGTTCATCATTACCGCCGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCACCTCGTATCCCTGTCGCAGTGAGGaa                                                                                                       >  6:28107/1‑135 (MQ=255)
                                atcatTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCTATCCAACTCGTATCGCTGTCTCAGTGAGGAatat                                                                                                    >  4:119278/1‑136 (MQ=255)
                                atcatTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCTATCCAACTCGTATCGCTGTCTCAGTGAGGAata                                                                                                     >  4:123741/1‑135 (MQ=255)
                                  catTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGTCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCGGTCTCAGTGAGGAATATc                                                                                                   >  4:51676/1‑135 (MQ=255)
                                  catTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATc                                                                                                   >  4:295736/1‑135 (MQ=255)
                                  catTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATc                                                                                                   >  5:189741/1‑135 (MQ=255)
                                  catTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATc                                                                                                   >  8:74316/1‑135 (MQ=255)
                                      ccAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATg                                                                                               <  3:293250/135‑1 (MQ=255)
                                      ccAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATg                                                                                               <  8:26891/135‑1 (MQ=255)
                                       cAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATg                                                                                               >  2:258911/1‑134 (MQ=255)
                                       cAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCAAACTCGTATCTCTGTCTCAGTGAGGAATATCGATg                                                                                               >  8:72175/1‑134 (MQ=255)
                                                atTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGGAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAg                                                                                    <  6:43077/136‑1 (MQ=255)
                                                atTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTTGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGaa                                                                                     <  1:60031/135‑1 (MQ=255)
                                                  tACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATAGATGTAGCGGTGAAGAc                                                                                  >  3:7294/1‑136 (MQ=255)
                                                      gctgctAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGCCTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTCGCGGTGAAGACTAgg                                                                              <  6:60150/136‑1 (MQ=255)
                                                          ctAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTc                                                                            <  3:37626/134‑1 (MQ=255)
                                                          ctAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCg                                                                          >  1:51998/1‑136 (MQ=255)
                                                           tAAAACTCATCAATAAAACTTCTATACATCGGCAAAAATTATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCgg                                                                         <  1:275668/136‑1 (MQ=255)
                                                            aaaCCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCgg                                                                         >  7:144950/1‑135 (MQ=255)
                                                             aaCCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCATTTATTAATATGGATGTAGCGGTGAAGACTAGGTCggg                                                                         <  2:256286/134‑1 (MQ=255)
                                                             aaCCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCgg                                                                         <  1:49304/134‑1 (MQ=255)
                                                             aaCCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGAAATCCAACTCGTATCGCTGTCTAAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCgg                                                                         >  4:267365/1‑134 (MQ=255)
                                                             aaCCTCATCAATAAAACTTCTAAACANCGGCAAAATTAATAGCTGTAAACCGACTGACNGATAGCTAGATGCAATCCAACACGTATCGCTGTCTCAGTGAGGAANATAGATGTAGCGGTTAAGACTAGGTCCgg                                                                         >  8:48510/1‑134 (MQ=255)
                                                                cTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAagga                                                                    <  3:287875/136‑1 (MQ=255)
                                                                     caATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAggg                                                                 >  2:61411/1‑134 (MQ=255)
                                                                     caATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAggg                                                                 >  8:169787/1‑134 (MQ=255)
                                                                       aTAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCTATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAgg                                                             >  7:245970/1‑136 (MQ=255)
                                                                       aTAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAgg                                                             >  5:211587/1‑136 (MQ=255)
                                                                            aCTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAAc                                                        <  7:65703/136‑1 (MQ=255)
                                                                            aCTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAAc                                                        <  3:251007/136‑1 (MQ=255)
                                                                                 tAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTg                                                   <  1:46809/136‑1 (MQ=255)
                                                                                   aaCATCAGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTg                                                   <  6:264477/134‑1 (MQ=255)
                                                                                         ggCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTa                                           >  6:147073/1‑136 (MQ=255)
                                                                                           cAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTAtt                                         >  6:79324/1‑136 (MQ=255)
                                                                                               aTTAATATCTGCTAACCGACTTACTGATAGCTCGATGCTATCCATCTCGTCTCGCTGTCTCAGTGTGGAATATCGATGTATCGGTCAAGACTAGGTCCGGAAGGAGGGTAGGGCATCATTTGGGCATGTATTTggg                                     <  4:164/136‑1 (MQ=255)
                                                                                                  aaTAGCTGGAAACCGACTGACTGATAGCTCGATGCTATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGTTGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGaa                                   <  3:123741/135‑1 (MQ=255)
                                                                                                  aaTAGCTGGAAACCGACTGACTGATAGCTCGATGCTATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGGTGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGaa                                   <  3:119278/135‑1 (MQ=255)
                                                                                                  aaTAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCTACAGTTGGGCATGTATTTGGGaa                                   <  7:59512/135‑1 (MQ=255)
                                                                                                  aaTAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGaa                                   <  5:169024/135‑1 (MQ=255)
                                                                                                  aaTAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGaa                                   <  8:160642/135‑1 (MQ=255)
                                                                                                   aTAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCATTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGAAAt                                 <  7:179260/136‑1 (MQ=255)
                                                                                                       cTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGAAATGGa                              <  6:211587/135‑1 (MQ=255)
                                                                                                             aCCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTATGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGTGAAATGGACTAc                          >  4:220467/1‑133 (MQ=255)
                                                                                                             aCCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGAAATGGACTACAAg                       <  7:147446/136‑1 (MQ=255)
                                                                                                             aCCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGAAATGGACTACAAg                       <  3:220263/136‑1 (MQ=255)
                                                                                                             aCCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGAAATGGACTACAAg                       <  3:51676/136‑1 (MQ=255)
                                                                                                                    gactgaTAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGaa                                   >  2:70953/1‑117 (MQ=255)
                                                                                                                    gactgaTAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGaa                                   <  1:70953/117‑1 (MQ=255)
                                                                                                                    gactgaTAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATAGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGTCAACAGTTGGGCATGTATTTGGGAAATGGACTACAAGACtgtg                 >  8:218117/1‑135 (MQ=255)
                                                                                                                         aTAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGAAATGGACTACAAGACTGTGGATa             <  4:23462/134‑1 (MQ=255)
                                                                                                                                 aTGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGAAATGGACTACAAGACTGTGGATAAATCTTTTgg   <  3:295736/136‑1 (MQ=255)
                                                                                                                                 aTGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGAAATGGACTACAAGACTGTGGATAAATCTTTTgg   <  8:59736/136‑1 (MQ=255)
                                                                                                                                   gCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGAAATGGACTACAAGACTGTGGATAAATCTTTTGGa  >  5:29864/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTCTAAACATCGGCAAAATTAATAGCTGGAAACCGACTGACTGATAGCTCGATGCAATCCAACTCGTATCGCTGTCTCAGTGAGGAATATCGATGTAGCGGTGAAGACTAGGTCCGGAAGGAGGGTAGGGCAACAGTTGGGCATGTATTTGGGAAATGGACTACAAGACTGTGGATAAATCTTTTGGA  >  ACB143/843567‑843832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑