Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143188,0120TA34.7% 40.6 / 25.1 49G957G (GGT→GGA) CJ019_00185hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (15/17);  new base A (0/17);  total (15/34)
Fisher's exact test for biased strand distribution p-value = 6.62e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCT  >  ACB143/187886‑188146
                                                                                                                              |                                                                                                                                       
cAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACACAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACCCCACAGGCGGT‑AATTTCGAg                                                                                                                               >  6:55535/1‑136 (MQ=255)
 aaCGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGa                                                                                                                                >  3:180205/1‑134 (MQ=37)
      ccAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACAGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTg                                                                                                                         >  1:268174/1‑136 (MQ=37)
                       aaGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGAcccc                                                                                                        >  1:291560/1‑136 (MQ=37)
                       aaGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACAGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAAACCGAccc                                                                                                         >  3:122864/1‑135 (MQ=21)
                        aGCCGTCAACACCCAGGGCAGCGTCGAATTCCAGACCCCGGCTAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCg                                                                                                       <  1:257642/136‑1 (MQ=255)
                         gCCGGCAAAACCCAGGGCAGCGTCGAGTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACAGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCgg                                                                                                      >  8:265685/1‑136 (MQ=21)
                              cAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGGCTACAACAACGGTTCGGCTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGACCCCGCCCCCGGCTCAg                                                                                                 >  6:177374/1‑136 (MQ=255)
                               aaaaCCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAAACCGACCCCGGCTCAGa                                                                                                >  8:41181/1‑136 (MQ=37)
                                           aGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGCGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACg                                                                                    <  7:141992/136‑1 (MQ=37)
                                           aGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCa                                                                                                                             <  3:77661/95‑1 (MQ=33)
                                           aGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAAACCGACCCCGGCTCAGa                                                                                                >  3:96593/1‑124 (MQ=25)
                                                cGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCg                                                                               >  7:64932/1‑136 (MQ=37)
                                                  aCTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCgg                                                                              >  4:180789/1‑135 (MQ=37)
                                                                          tCTACAACAACGTTTCGACGGTCAGCGGCACCATCCACAACGCAACCGGCGTA‑AATTTCGAGCACATGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGAc                                                     <  1:25633/136‑1 (MQ=11)
                                                                              caacaaCGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAAGCCACGACCGCGACCCTGACGGc                                                  >  2:251716/1‑135 (MQ=21)
                                                                                                  gcgcCACCATCCACACCCCCACTGGGGGA‑AATTTCCACCAACTCACCCCCAACCCGACCCCGGCCCAGACCACCATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAg                              <  3:190820/135‑1 (MQ=11)
                                                                                                  gCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAgg                             >  6:91927/1‑136 (MQ=32)
                                                                                                  gCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAGGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAg                              >  3:95699/1‑135 (MQ=17)
                                                                                                    gTCACCATCGACACGGCCCCTGGCGTT‑ATTTTCGAGCACCTCACCCCGAACCCGACCCCGGCTCACCCCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg                           <  6:250078/136‑1 (MQ=11)
                                                                                                     tCACCATCGCCAACCCCACGGGCGTA‑AATTACGACCAACTGACCCCCAACCCGACCCCGGCGCAGACCCCCATCACCGACTCGGTCGAAGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg                           <  4:239959/135‑1 (MQ=11)
                                                                                                     tCACCATCGACAACGCCACAGGCGGT‑AATTTCGCGCAACTGACCCCGAACCCGACCCCGCCTCAGACCCCGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg                           <  4:316521/135‑1 (MQ=255)
                                                                                                      cacaaccGAGACCGCCACCGGCGTA‑AATTTCGCCCAACTCCCCCCCAACCCGCCCCCGGCCCACACCACGACCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  6:320706/130‑1 (MQ=11)
                                                                                                      cACCATCGAGACCGCCCCGGGGGGA‑AATTCCTACCACCGGACCCCGAACCCGCCCCCGGCTCAGCCCCCTATCAACGACTCGGTCGATCCCACAACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  8:306764/136‑1 (MQ=11)
                                                                                                      cACCATCGAGAACGCCACCGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  2:61302/136‑1 (MQ=17)
                                                                                                      cACCATCGACACCGCCACCGGGGGT‑AATTTCGACCACCTGCCCCCGAACCCGCCCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  5:89492/136‑1 (MQ=11)
                                                                                                      cACCATCGACACCGCCACCGGCGGA‑AATTTCGACCACCTCACCCCTAACCCGCCCCCGGCCCACACCACGAACAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  6:1058/136‑1 (MQ=11)
                                                                                                      cACCATCGACACCGCCACAGGCGTT‑AATTTCGAGCAACTGCCCCCCAACCCCACCCCGGCCCCGCCCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  7:312871/136‑1 (MQ=11)
                                                                                                       caaaacGAGAACGCCACTGGCGGA‑AATTTCCACCACCTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  2:55149/130‑1 (MQ=11)
                                                                                                       accaccGACACCCCCACAGGGGGT‑AATTACGACCAACTCACCCCCAACCCGACCCCGGCTCCCACCACTATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  3:49559/135‑1 (MQ=11)
                                                                                                       aacATCTAGAACCCCACCGGCGTA‑AATTTCGACCACATCACCCCGAACCCGCCCCGGGCTCACACCACTATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  4:183869/133‑1 (MQ=11)
                                                                                                       aCCATCGAGAACGCCACCGGCGGA‑AATTTCCACCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGAACAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  6:180348/135‑1 (MQ=11)
                                                                                                       aCCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  7:269914/135‑1 (MQ=32)
                                                                                                       aCCATCGACAACGCCACCGGCGTA‑AATTACCACCAACTCACCCCGAACCCGACCCCGGCGCACACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  4:373356/135‑1 (MQ=11)
                                                                                                       aCCATCCAGAACGCCACAGGCGGA‑AATTTCGAGCAACTCCCCCCGAACCCCCCCCCGGCTCAGACCACGATCCACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                         <  4:58273/135‑1 (MQ=11)
                                                                                                        ccATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt                       <  8:292598/136‑1 (MQ=32)
                                                                                                        ccATCGACACCGCCACCGGCGGA‑AATTGCGACCACCTCCCCCCCAACCCGCCCCCGGCTCAGACCACGAGCAACGACTCGGGCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt                       <  2:45129/136‑1 (MQ=11)
                                                                                                             gagaACGCCACCGGCGTA‑AATTTCCACCACCTCCCCCCGAACCCGACCCCGGCACACACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATc                   <  6:141823/135‑1 (MQ=11)
                                                                                                             gagaACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATc                   <  7:27565/135‑1 (MQ=32)
                                                                                                              agaACGCCAC‑CGCGGTAAATTGGGAGCAACTGACCCCGAACCCGACCGCGGCTCAGACCACGATCAACGACTGGGTCGATGCCACCACCGCTACCCTTACGGCGAGCCCGTCGGTCACGGAAGGGGGCGTTATCAc                 <  2:259395/136‑1 (MQ=11)
                                                                                                               gaACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAc                 <  8:64932/135‑1 (MQ=32)
                                                                                                               gaACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAc                 <  3:316071/135‑1 (MQ=32)
                                                                                                                aaCGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAATCCACAACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAc                 >  3:79472/1‑134 (MQ=12)
                                                                                                                   gCCACTGGCGGA‑AATTTCGCCCACCTCCCCCCGAACCCGCCCCCGGCTCCGACCACGAACAACGACTCGGGCGATGCCACCCCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTaca            <  1:316967/136‑1 (MQ=11)
                                                                                                                   gCCACTGGCGGA‑AATTTCGACCACCTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTaca            <  8:108504/136‑1 (MQ=255)
                                                                                                                    cccccggCGTT‑AATTTCGAGCACCTGACCCCGAACCCGACCCCGGCACACACCCGTATCAACGACTCGGGCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTaca            <  4:18286/130‑1 (MQ=11)
                                                                                                                    ccACAGGCGGT‑AATTTCCACCAACGCCCCCCAACCCCGCCCCCGCCTCACACCACTATCAACGACTCGGGCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTaca            <  4:138307/135‑1 (MQ=11)
                                                                                                                           cGGA‑AATTTCGACCACCTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTg       <  4:311218/133‑1 (MQ=12)
                                                                                                                           cGGA‑AATTTCGACCACCTCACCCCGAACCCGACCCCGGCTCAGACCCCGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTg       <  3:128671/133‑1 (MQ=11)
                                                                                                                              t‑AATTTCCAGCAACTCACCCCTAACCCGCCGCCGCCGCTTAACACCAACAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCt  <  4:111220/135‑1 (MQ=11)
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CAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGT‑AATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCT  >  ACB143/187886‑188146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑