Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143452,9400GC32.3% 107.8 / 16.5 65intergenic (‑36/+274)pqqB/CJ019_00410Coenzyme PQQ synthesis protein B/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/14);  new base C (0/20);  total (28/37)
Fisher's exact test for biased strand distribution p-value = 1.77e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGA  >  ACB143/452817‑453075
                                                                                                                           |                                                                                                                                       
cATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAAACCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCgg                                                                                                                             >  8:8125/1‑136 (MQ=255)
 aTCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCgg                                                                                                                             >  7:196662/1‑135 (MQ=255)
 aTCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCgg                                                                                                                             >  2:10355/1‑135 (MQ=255)
        tAGCCCTTGCAGTTTACGCAGTTGCAGTTCCAATGCGGGAACCCGCCACCGGCTGCGGAGCCGAGAACCTGGATGTACATGGACACTCTCTATGCAGAACCCGTATCGAAAAACCGAAAACGCACCGGCAGGcc                                                                                                                       >  6:241617/1‑134 (MQ=255)
            ccTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGAAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGc                                                                                                                  >  4:235854/1‑135 (MQ=255)
            ccTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGCCCACTCTCTATGCACAAACCGGATCCAAAAAACGAACACGCCCCGGCAGGCCGAGGCg                                                                                                                 <  5:301576/136‑1 (MQ=255)
                  aGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAAc                                                                                                           >  8:286781/1‑136 (MQ=255)
                   gTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGCGGCGTGAAAc                                                                                                           >  4:97631/1‑135 (MQ=255)
                   gTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAAcc                                                                                                          >  7:315633/1‑136 (MQ=255)
                       aCGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAAccc                                                                                                         >  4:279587/1‑133 (MQ=255)
                       aCGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCt                                                                                                        >  5:90818/1‑134 (MQ=255)
                         gcagttgcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAg                                                                                                     >  4:202167/1‑135 (MQ=255)
                                   ccccccTGCGGGACCCCGCACCCGGGGGCGGAGCCCACACCCTCGCTGACCATGCCCCCTCTCTTTCCCCAACCCGCACCCAAAACACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGc                                                                                          <  7:156567/131‑1 (MQ=255)
                                    tcaaCTGGGGTACCCCGCCACGGGCGGCGGAGCCGACAACCGGGAGTTACAGGCCCAGTCACTATCCACAACCCGGATCGAAAAACCCAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGc                                                                                          <  6:289921/132‑1 (MQ=255)
                                       aCTGCGGGAACCCGCCACCGGCGGCGCAGCCGAGAACCTGGATGTACATGCCCACTCTCTATGCACAACCCGGATCCAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGtt                                                                                      <  6:10450/136‑1 (MQ=255)
                                        ccgCGGGAACCCGCCCCGGGCGGCGGAGCCGAGAACCTGCTTGTACATGGCCACGCACTATGCAGAACCCGGATCGAAAAACCCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa                                                                                     <  2:285085/134‑1 (MQ=255)
                                        cTGGGGGAACCCGCCACCGGCGGCGTACCCGAGAACCTGGATGAACTTGGCCACTCTCTATGCAGAAACCGTACCCAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa                                                                                     <  8:55818/136‑1 (MQ=255)
                                        cTGGGGGAACCCGCACCCGGGGGCGGAGCCGATAACCTGTTTTTACATGGCCACTCTCTTTGCAGAAACCGCATCCAAAAAACCAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa                                                                                     <  2:55122/136‑1 (MQ=255)
                                        cTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCATAAACCGGATCGAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa                                                                                     <  6:355468/136‑1 (MQ=255)
                                          gCGGTACCCCGCCCCGGGGGGGGGGGCCTAGAACGTGGTTGTACTGGGCCACTCTCTATCCACACCCCGGAGCTAAAAACCCACAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAg                                                                                    <  4:8315/135‑1 (MQ=255)
                                          gCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGc                                                                                   >  8:203332/1‑136 (MQ=255)
                                          gCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGACCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGc                                                                                   >  8:324615/1‑136 (MQ=255)
                                           gggcaccccGCCACCGGCGGCGGCGCCGATAACCTGTATGTACATGGCCACTCTATATCCACAACCCGCAACTAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAg                                                                                    <  5:109226/128‑1 (MQ=255)
                                              gAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAg                                                                               >  1:55122/1‑136 (MQ=255)
                                                aCCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTa                                                                             >  1:203697/1‑136 (MQ=255)
                                                   cGCCACCGGGGGCGGAGCCGAGAACCTGGATGTACAGGGCCACTCTCTATGCAGAACCCGGAACCAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACAt                                                                          <  8:26761/136‑1 (MQ=255)
                                                   cGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACAt                                                                          <  1:40609/136‑1 (MQ=255)
                                                              gcggAGCCGAGAACCTGGATGTACAGGTCCACTCTCTATGAAGAAACCGGATCCAAAAAACCAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGa                                                               <  1:118078/136‑1 (MQ=255)
                                                              gaccggCCGATAACGTGGTTGTACATGGCCACTCTCTATGCAGAACCCGTATCGAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGa                                                               <  2:51970/131‑1 (MQ=255)
                                                                     cgtgaACATGGATGTACATGGCCACTCTCTATGCATAAACCGGATCTAAAAAACTAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGAt                                                        <  7:179426/133‑1 (MQ=255)
                                                                     cGAGAACCTGGATGTACATGGCCACTCTCAATGCACAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGAt                                                        <  2:250088/136‑1 (MQ=255)
                                                                     cGAGAACCTGGAGTTACATGGCCACTCTATATGCAGAACCCGGATCCAAAAACCGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGAt                                                        <  7:123973/136‑1 (MQ=255)
                                                                      gagaACCTGTATGTACATGGCCACTCTCTATGCAGAAACCGGATCTAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGAt                                                        <  4:350274/135‑1 (MQ=255)
                                                                         acccTGTATGAACAGGCCCATACTCTAGGAACAAACCGGATCCAAAACCCCAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGc                                                    <  6:249528/134‑1 (MQ=255)
                                                                          cccTGTATTTACCTGCCAACTGTATATGAATAACCTGGATCTAAAAACCCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCa                                                   <  6:304507/135‑1 (MQ=255)
                                                                          aacTGTATGAACATGCCCAATCTCTAACCAAAAACCTCATAAAAAAACACAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCa                                                   <  1:262572/134‑1 (MQ=255)
                                                                          aCCTGGATGTACATGGCCCCTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCa                                                   <  8:169366/136‑1 (MQ=255)
                                                                          aCCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCa                                                   <  2:224200/136‑1 (MQ=255)
                                                                          aCCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCa                                                   <  2:11624/136‑1 (MQ=255)
                                                                           cgtGGATGTACATGCCCAATCTATATGCAGAAACCGTATCCAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCa                                                   <  3:139183/133‑1 (MQ=255)
                                                                            catgATGTACATGCCCACTATATATCCAAAAACCGGAACAAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAgg                                                 <  2:163423/133‑1 (MQ=255)
                                                                             tgtatttACATGCCCACTCTCTAACCCTAACCCGTATCAAAAAACCCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAgg                                                 <  4:223085/129‑1 (MQ=255)
                                                                                 tGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAggctgtc                                            >  1:51970/1‑131 (MQ=255)
                                                                                    acttGGCCACTCTATATGCATAAACGGTATCCAAAAAACTAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAgtgt                                          <  5:311693/132‑1 (MQ=255)
                                                                                             ctctctATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAggctg                                >  1:163423/1‑133 (MQ=255)
                                                                                             ctctcaATGCCTAACCCGGAACCAAAACCCCACAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGttt                                <  5:311600/136‑1 (MQ=255)
                                                                                              tgtatATGCAGAAACCGGATCTAAAAAACTAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGttt                                <  4:77697/131‑1 (MQ=255)
                                                                                                tctATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGACGAGGCGTGAAACCCTAAGCCTGGCTAAGCGGATATCGAACTAAATGGAGACTTAGAATCCGATACGCAGGTCATTGTATCCAGGTTTgct                             >  5:15944/1‑134 (MQ=255)
                                                                                                  tATGCATAACCCGGATCGAAAAACCGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTcc                           <  7:8125/136‑1 (MQ=255)
                                                                                                  tATGCAGAACCCGAATCGAAAAAACGAAAACGCCACGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTcc                           <  5:120768/136‑1 (MQ=255)
                                                                                                  tATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTcc                           <  7:17331/136‑1 (MQ=255)
                                                                                                   atccacaaaCCGGATCCAAAAAACGAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCACTACGCAGGTCAGTGTATGCAGGTTTGGTcc                           <  3:27685/129‑1 (MQ=255)
                                                                                                     gCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCcac                         >  3:190010/1‑135 (MQ=255)
                                                                                                     gCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCcac                         >  3:228989/1‑135 (MQ=255)
                                                                                                     gCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCAAAGACTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCcac                         >  3:197715/1‑135 (MQ=255)
                                                                                                      cacaacccGCATCCAAAAACCCAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACAt                       <  5:56295/130‑1 (MQ=255)
                                                                                                       aGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATg                      >  6:30176/1‑136 (MQ=255)
                                                                                                           aCCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGaa                   >  4:253562/1‑135 (MQ=255)
                                                                                                           aCCGAATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTTAAACCCTAACCCTGGCTTAGCGGTTAGCGAAGTACATGGTCACTTAGAAGCCGATACTCACGTAAGTGAATGAAGCTTTCGTCCACAtctaat                  >  2:92640/1‑131 (MQ=255)
                                                                                                            ccGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGaa                   >  3:190736/1‑134 (MQ=255)
                                                                                                            ccGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAAt                  >  6:295187/1‑135 (MQ=255)
                                                                                                            ccGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGCCGAGAAACCCTAAGCCTCGCTTAGCGGATAGCGAAGTACAAGGTGACTTCGAAGCCGATACGCAGGTCAGTGAATGCAGGTTTGGACCACAAGGAAt                  >  6:135808/1‑135 (MQ=255)
                                                                                                            ccGGAGCGAAAAAACGAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAAtt                 <  8:341147/136‑1 (MQ=255)
                                                                                                             cGGATCTAAAAAAAGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGCTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAAtt                 <  4:293133/135‑1 (MQ=255)
                                                                                                               gATCGAAAAAACGAAAACGCCCCTGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTAGAATACGATACGCAGGTCAGTGTATGCAGGTTTGGTACACATGGaagttct              >  8:311006/1‑131 (MQ=255)
                                                                                                                      aaaaccaaaacgCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCg       <  5:213537/136‑1 (MQ=255)
                                                                                                                       aaacgaaaacgCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCg       <  4:190736/135‑1 (MQ=255)
                                                                                                                           gaaaacgCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGa  <  8:27823/136‑1 (MQ=255)
                                                                                                                           |                                                                                                                                       
CATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGA  >  ACB143/452817‑453075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑