Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143994,2460AT52.4% 6.8 / 15.3 26Q58L (CAG→CTG) CJ019_00938hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/10);  new base T (0/11);  total (1/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCA  >  ACB143/994124‑994378
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ggCCATGCGCGCGGAAACTCCGGGCAAGGCCGCCCTGGCGATTCTGGCGGCAGCGGGGCCGGGGGTGGGCGAGGCGCTGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGGGGCATCCAGCAGGATGCCACGGtt                                                                                                                         <  1:67993/136‑1 (MQ=255)
ggCCATGCCCGATCCAACCCCCGGCAAGCCCCCCCCGGCTATGCGGGCGGCAGCGGGGCGGGGGGGGGTCCCGGCGCCGTTGCTGCGGGGGCAGCTCCTGCTGGATGGGCGTGGAATCCAGCAGGATGCCACGGtt                                                                                                                         <  6:346812/136‑1 (MQ=255)
       cTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCCGGATGCCCCGGTTTCCCGGc                                                                                                                  >  6:138880/1‑136 (MQ=255)
           tttaaccTCCCGGCAAGGCCGCCCTGGCGATTCTGGGGGCAGGGGGGGTGGGGGGGGTCGAGGCGCAGTTGCGGCGGGGGCATTTCGGGCTGGTGGGGCGGGGCATCCAGCTGGATGCCACGGTTGCCCGGCGTTg                                                                                                              <  8:239600/130‑1 (MQ=255)
              aaaCTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGGGTGGGCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGGGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGtt                                                                                                           <  6:361920/136‑1 (MQ=255)
               accTCCGGGCATGGCCGCCCAGGCTATGCTGGCGGCAGCGGGGCAGGGTGTGGGCGTGGCGCAGTTGCGGCTGGGGAAGATCCTGCTGGAGGGGCGTGGCATCCTGCAGGATGCCACGGTTGCCCGGCGTTGGtt                                                                                                           <  3:300090/133‑1 (MQ=255)
               aaCTCCGGGCACGGCCGCCCGGGCGTTTGGGGGGGCAGGGGGGGTGGGGGGGGGCGAGGGGCTGTTGGGGGGGGGGCAGATCGTGCGGTTTGGGCGTGGCATCCAGCTGGATGCCACGGTTGCCCGGCGTTGGtt                                                                                                           <  8:154499/135‑1 (MQ=255)
                  tCCCGGCACGGCCGCCCTGGGTATTCTGGCGGCAGGGGGGCTGGGTGTGGGCGAGGCGCAGTTGGTGGGGGGGCAGATCCTGGTGTTTGGGCGGGGAATCCCGCAGGATGCCACGGTTGCCCGGCGTTGGTTCgg                                                                                                        <  4:155937/135‑1 (MQ=255)
                        ccaGGCCGCGCTGGCGTTTGTGGGGACCGAGGGGGTGGGGGGGGGCGTGGGGCTGTTGGTGGTGGGGCTGATCCTGTGGGTGGGGCGGGGCTTGATGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                                 <  2:6007/134‑1 (MQ=255)
                         acgcccgccCCGGGGATTGTGGCGGCTGGGGGGCTGGGTGTGGTCGAGGCGCTGTGGGTGCGGGGGCTGAGCCTGCTGGAGGGGCGGGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                                 <  4:89658/131‑1 (MQ=255)
                         aaGGCCGCCCAGGCGTTTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCGACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                                 <  4:61716/135‑1 (MQ=255)
                            gccgccCAGGCGATGCGGGCGGCAGCGGGGCAGGGGGGGGGCGAGGCGCAGTGGCTGCGGGGGACGAGCCTGCTGGCGGGGCGTGGCATCCAGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGcac                                                                                             <  2:284009/136‑1 (MQ=255)
                                  cAGGCGATTCTGGCGGCAGCGGGGCTGGGTGGGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGt                                                                                        <  3:274098/135‑1 (MQ=255)
                                        aTTCTGGCGGCCGCGGGGCAGGGTGGGGGCGAGGCGCCGTTGCTGCTGGGGCAGATCCTGCGGGAGGGGCGTGGCACCCCGCCGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTg                                                                                 <  1:33941/136‑1 (MQ=255)
                                             ggcggcTGGGGGGCCGGGTGGGGGCGGGGCGATGTTGCGCCTGGGCCCCATCCGGGTGGTGGGGGGGGCCACCCAGCTGCATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATg                                                                            <  8:5702/136‑1 (MQ=255)
                                             ggcggcAGCGGCGCTGGGGGGGGTCGAGGCGCTGTTGGGGGTGGGGCATTCCCTGGTGTTGGGGGTTGGAATCATGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATg                                                                            <  8:182285/136‑1 (MQ=255)
                                                 gcAGCGGGGCAGGGTGTGGGCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGgcgc                                                                        <  1:115145/136‑1 (MQ=255)
                                                 cctgCGGGGCCGGGGGGGGTCGAGGCGCAGTGGCGGGTGGGGCAGATCCTGGTGGATGGGCGGGGCACCCCGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGgcgc                                                                        <  7:297717/133‑1 (MQ=255)
                                                         gCAGGGTGGGGTCGAGGCGCAGTTGGTGCTGGGGCTTATCGTGCTGGATGGGCGTGGCATCCAGATGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACAt                                                                 <  4:311389/135‑1 (MQ=255)
                                                              gtgtGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCgg                                                           <  8:292230/136‑1 (MQ=255)
                                                                      ctggCGCAGTTGCGGGGGGGGCAGATCCGGGGGGTGGGGGGGGCCACCCCGCCGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGc                                                    <  3:189498/133‑1 (MQ=255)
                                                                        ggCGCAGTTGCGGCTGGGGAATACCCGGCGGGAGGGGGGTGCCAACCAGCCGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTg                                                 <  5:307233/136‑1 (MQ=255)
                                                                                  gtgctgGGGCAGATCCTGCGGTTTGGGCGTGGCATCCAGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCt                                       <  2:260393/135‑1 (MQ=255)
                                                                                     ctgGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTggg                                    <  7:269916/136‑1 (MQ=255)
                                                                                             gagccTGGTGGTGGGGGGGGGCACCCAGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGa                             <  4:135351/132‑1 (MQ=255)
                                                                                                                 ccacccAGCCGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAggcgg         <  3:126027/131‑1 (MQ=255)
                                                                                                                       agcagGATGCCACGGTTGCCCGGCGTTGGGTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCa  <  2:114741/136‑1 (MQ=255)
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GGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCA  >  ACB143/994124‑994378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑