Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,113,8950TG35.1% 47.6 / 10.8 39R663R (CGT→CGG) CJ019_02007hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/23);  new base G (13/0);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 3.93e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTCGCTGAGGGCGATGGCAGGCGTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCC  >  ACB143/2113779‑2114013
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gtcgCTGAGGGCGATGGCAGGCGTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTa                                                                                                      <  7:245890/135‑1 (MQ=255)
    cTGAGGGCGATGGCAGGCGTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCa                                                                                                 <  7:58666/136‑1 (MQ=255)
    cTGAGGGCGATGGCAGGCGTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCa                                                                                                 <  6:88841/136‑1 (MQ=255)
              tGGCAGGCGTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCGCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCt                                                                                       <  1:52940/136‑1 (MQ=255)
                  aGGCGTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGaa                                                                                    <  3:289230/135‑1 (MQ=255)
                      gTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTCAATAGATTTTCTGGTACGCCCGCGGCGGCCAGAAATGGt                                                                               >  6:132167/1‑136 (MQ=255)
                                taCCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACg                                                                       <  2:34828/134‑1 (MQ=255)
                                  ccGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGGGAATATATTTTGTGGTTCGCCCGCGGCGGCCGGGACTGGTGGTCGGCGGCa                                                                    >  5:119576/1‑135 (MQ=255)
                                        gcagTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGGATATAATTGGTTGTTCGGCCGCGGCGGGCGGGAATGGTTTTCGACCTAAAGCAGtt                                                             >  1:356735/1‑136 (MQ=255)
                                        gcagTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATCTATTTTCGGGTACGCCACCCGGGGCCGGGACTGGTGGTAGACGTCAACCAGt                                                              >  4:128634/1‑135 (MQ=255)
                                        gcagTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATATATGTGGTGGTTCGCCCGCGGCGGGCGGGACTGGTGATGGCCCGCAAGCAGtt                                                             >  5:76061/1‑136 (MQ=255)
                                        gcagTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGGGAATATATGTGGTGGGTCGCCACCGGCGGCCGGAACTGGGGGTAGACGTGAAGCAGt                                                              >  3:88763/1‑135 (MQ=255)
                                          agTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATCTATGTGGTGGTAAGCCAGCCGCGGCCGGAACTGGTGGTCGACGGCAAGCAGTTGc                                                           >  7:262138/1‑136 (MQ=255)
                                          agTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATATATTTGCTGGTTCGCCCGCGGCGGCCGGGAAAGGTGATCGACGGCAAGCAGTTgg                                                           >  1:285099/1‑135 (MQ=255)
                                             aaGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTAAATATATGTGGGGGGAACCCCGCGGCGGGCTGAACTGGTGATAGAACTCACACAGtttgccg                                                        >  7:97320/1‑131 (MQ=255)
                                                   gATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGGGATCGACGTCAAGCAGTTGCCCGATGGc                                                   <  1:150594/135‑1 (MQ=255)
                                                     tGCCAACGGGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGCGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGAc                                                <  5:72880/136‑1 (MQ=255)
                                                      gCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGCAACTATGTGGTGGTTAGCCCGCGGCGGGCGGCACAGGGGTTAGGCGGAAAGCAGTTGGCCCAAGGCGccc                                               >  5:342300/1‑136 (MQ=255)
                                                      gCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATCTATGTTCTGGTTCGCCAGCGGCGGGCCGAACGGGTGGTAGACGGCAAAGAGGTTCCCGATGGCgcc                                                >  3:344481/1‑133 (MQ=255)
                                                       ccAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCt                                              <  8:30213/136‑1 (MQ=255)
                                                         aaCGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGCTCAATCTATGTGCTGGTAAGCCAGCGGCGGCCTGGAATGGTGATCGCCGTCAAGCAGTTGCCCGATGGCGACCTg                                             >  2:333434/1‑135 (MQ=255)
                                                            gTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGt                                         <  1:279382/136‑1 (MQ=255)
                                                            gTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGt                                         <  6:22847/136‑1 (MQ=255)
                                                            gTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGt                                         <  6:52453/136‑1 (MQ=255)
                                                            gTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGt                                         <  7:144720/136‑1 (MQ=255)
                                                             tGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGt                                         <  3:180214/135‑1 (MQ=255)
                                                             aggCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGt                                         <  3:258305/134‑1 (MQ=255)
                                                                 cTACGTGCTCGAGCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACa                                    <  8:279080/136‑1 (MQ=255)
                                                                    cGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACatg                                  <  8:230631/135‑3 (MQ=255)
                                                                           gATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGtt                            <  1:22211/134‑1 (MQ=255)
                                                                           gATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGtt                            <  5:186771/134‑1 (MQ=255)
                                                                            aTCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACATTCATTTGACAGGTATGtt                            <  3:58329/133‑1 (MQ=255)
                                                                             tcaggtcaggGTACAGAACCGGGAGACCAGCGAGTCGCGGTCAATCTATGTGCTGGTTAGCCCGCGGCGGCCTGAACAGGTGATAGACGTCAAGCAGTTGCCCCATGGCGCCCAGCAGTTTACAGGAAAGttact                         >  4:258305/1‑132 (MQ=255)
                                                                                   aggggTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTa                  <  5:113927/134‑1 (MQ=255)
                                                                                            gAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGgccagcca         <  6:76061/136‑1 (MQ=255)
                                                                                                  ggAGACCAGCGAGTCGCGATCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACAcc   <  7:291086/136‑1 (MQ=255)
                                                                                                   gagaCCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACAccc  <  5:72568/136‑1 (MQ=255)
                                                                                                   gagaCCAGCGAGTCGCGGTGAATCTATGTGGTGGTTCGCCCGCGGCGGCCGGGACTGGTGATCGACGTCAAGCACTTTGCCCATTGCGCCCGGCAGTTGACAGGTTTGTTCGATGGGGTAGGGcacccacccacca  >  6:113927/1‑135 (MQ=255)
                                                                                                   gagaCCAGCGAGTCGCGGTGAATATATGTGCTGGTTCGCCAGCGGCGGCCTGAACAGGTGGTCGCCGTCAACCAATTTCCCCATGGCGCCCCGCAGTTGGCAGGGATGTTGGATGGGGTTGGCCAGCCACACcccc  >  8:13816/1‑136 (MQ=255)
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GTCGCTGAGGGCGATGGCAGGCGTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCC  >  ACB143/2113779‑2114013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑