Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,641,8260AC31.2% 84.2 / 10.3 48Q36P (CAG→CCG) CJ019_02499hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/31);  new base C (15/0);  total (17/31)
Fisher's exact test for biased strand distribution p-value = 1.24e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCCCCCCGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTGTGCCGTGCCATCC  >  ACB143/2641696‑2641954
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cgcCCCCCGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGTTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTc                                                                                                                              <  5:1460/135‑1 (MQ=255)
   cccccGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCGCGGGc                                                                                                                          >  1:161295/1‑136 (MQ=255)
      ccGTTTCGAGTGCTCCTAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTgg                                                                                                                       <  5:58458/136‑1 (MQ=255)
             gAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTg                                                                                                                  <  4:353522/134‑1 (MQ=255)
             gAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTg                                                                                                                  <  3:280397/134‑1 (MQ=255)
             gAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGcc                                                                                                                <  7:181215/136‑1 (MQ=255)
             gAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGcc                                                                                                                <  5:227298/136‑1 (MQ=255)
                  cTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCACCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCAGCGAGGCCAGGGCGTGCCGGTCg                                                                                                           >  6:73100/1‑136 (MQ=255)
                     cAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGAc                                                                                                         <  4:42553/135‑1 (MQ=255)
                      aaaTGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCCCGCGCGCCGGGCGGGCCGGtgggcc                                                                                                        >  4:329606/1‑130 (MQ=255)
                        aTGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGAAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCGCAAGACCCGGGCGAGCCGGGCGAcccga                                                                                                     >  8:66449/1‑133 (MQ=255)
                               gcCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGCGGGGGGCGTGGCGGGGGCCCGGACGTtgt                                                                                               >  4:341172/1‑135 (MQ=255)
                               gcCCTGCCTGGTCCCTGTATGCCTATCAACTGATCAAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCCCGCCGGCGGGGCGGGCCGGGCGCCCGGACGTtgg                                                                                               >  3:233100/1‑134 (MQ=255)
                                     cctgGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTggcgg                                                                                        <  2:299093/136‑1 (MQ=255)
                                      ctgGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGCGCTGGGCGTGCCGGTCGCCCGTACTTTGTGTtggggc                                                                                       >  1:135228/1‑136 (MQ=255)
                                      ctgGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTggcg                                                                                         <  4:318648/134‑1 (MQ=255)
                                                  tGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGCGCTGGGCGGGCCGGTCGCCCGGACTTTGTGTGGCGGCCTGCTGCCAGcg                                                                           >  5:143161/1‑135 (MQ=255)
                                                  tGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCCCGCGCGCGGGGGGGGCCGGGCGGCCGGACGTTTTGTTGCGGCCGGCGGCCCGc                                                                            >  4:359747/1‑135 (MQ=255)
                                                    ccgaTCAACTGCTCGAGCCCGCCGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCa                                                                         <  6:219865/133‑1 (MQ=255)
                                                    ccTATCAACTGCTCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTc                                                                          <  4:38597/135‑1 (MQ=255)
                                                       aTCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGcc                                                                      <  1:240876/136‑1 (MQ=255)
                                                         cAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGcc                                                                      <  3:14331/134‑1 (MQ=255)
                                                         cAACTGATCGAGCCCGACGAGCAGCTCGACCTGGTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGcc                                                                      <  3:271076/134‑1 (MQ=255)
                                                          aaCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCCCTCGCCCGGGGCGGGGCCGTCGACCGGACTTTTTGTTGCGGCCAGCGGCCCCCGCAGCcggc                                                                   >  8:152941/1‑134 (MQ=255)
                                                           aCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCCCGCGAGCGGGGCGGGCCGGTGGGCCGGACATTGTGTGGGGGCCGGCGGCCCCCTCCGCcccg                                                                   >  3:123980/1‑135 (MQ=255)
                                                                  gAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGAGCGGGGCGTGGCGGGCGGCCGTACTTTTTGTGGCGGGCGGCGGCCCGCGCCTCCGCGGTGGTggg                                                           >  2:41801/1‑136 (MQ=255)
                                                                  gAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCGCGCGCGGGGCGGGGCGGGCGCCCGGACGTTGTGTGGGGGGCGGCGGGCCGCGCAGCCCCGGTGGTggg                                                           >  5:66027/1‑136 (MQ=255)
                                                                  gAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCGCGACCGGGGCGGGGCCGGCGGCCGGACGTTGTGTTGCGGCCGGGGGCCCGCGCAGCCGCGGTggggg                                                            >  3:351305/1‑132 (MQ=255)
                                                                        gacgaGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCGCGCGCTGGGGGGGCCGGGCGCCCGGACGTTGTGTGGCGGGCGGCTGCCAGCGCAGCCGCGGTGGTGGGGGGGGc                                                     >  8:234314/1‑136 (MQ=255)
                                                                              cagcTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGTGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGcga                                                <  3:140680/135‑1 (MQ=255)
                                                                                 cTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGAGCTGGGGCTGCCGGTCGCCCGTACGTTGTGTGGCGGCCTGCAGCCAGCTCAGCCCCGGTGGTCGGGGGTGCCGcgctcga                                            >  8:64085/1‑136 (MQ=255)
                                                                                              ccTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGAt                                 <  3:45312/134‑1 (MQ=255)
                                                                                              ccTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATgg                               <  2:230278/136‑1 (MQ=255)
                                                                                              ccTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATgg                               <  7:293334/136‑1 (MQ=255)
                                                                                                    aGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGcc                          <  3:251831/135‑1 (MQ=255)
                                                                                                    aGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGcc                          <  4:312938/135‑1 (MQ=255)
                                                                                                       aaaGCCGCGTCCACCTCGTGGCCCGCCCGCGGGCGGGGGGGGGGCCGGGGGGCCGGTCGTTTTGTGGCGGCCTGCGGGCAGCGCAGCCGCGGTGGTGGGGGGGGCCGCGGGCGGTCGACCCGGAGGGTCGcgggt                       >  4:62456/1‑131 (MQ=255)
                                                                                                        aaGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTtgt                     <  1:296541/136‑1 (MQ=255)
                                                                                                        aaGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTtgt                     <  6:84632/136‑1 (MQ=255)
                                                                                                        aaGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTtgt                     <  1:313619/136‑1 (MQ=255)
                                                                                                         aGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTtgt                     <  3:342948/135‑1 (MQ=255)
                                                                                                                  cACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTgtgcc           <  2:279946/136‑1 (MQ=255)
                                                                                                                    ccTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGTCCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTgtgccgt         <  6:293599/136‑1 (MQ=255)
                                                                                                                    ccTCGGGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTgtgccgt         <  8:175803/136‑1 (MQ=255)
                                                                                                                     cTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTgtgccgt         <  3:370377/135‑1 (MQ=255)
                                                                                                                      tCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTgtgccgtg        <  4:83147/135‑1 (MQ=255)
                                                                                                                         tGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTGTGCCGTGCCAt    <  8:44569/136‑1 (MQ=255)
                                                                                                                           gCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTGTGCCGTGCCATcc  <  5:366438/136‑1 (MQ=255)
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CGCCCCCCGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTGTGCCGTGCCATCC  >  ACB143/2641696‑2641954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑