Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	3,138,350	0	T	G	35.7%	69.2 / 19.7	56	F240V (TTT→GTT)	rbsC_2	Ribose import permease protein RbsC
Reads supporting (aligned to +/- strand):  ref base T (7/29);  new base G (20/0);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.13e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGGCA  >  ACB143/3138221‑3138484                                                                                                                                  |                                                                                                                                       acGCCATTCGGCCTGCGTCTGTACGCNATCGGNGACTACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGtt                                                                                                                                  <  2:173527/136‑1 (MQ=255) acGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGtt                                                                                                                                  <  6:77116/136‑1 (MQ=255)      aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTgggcg                                                                                                                             >  1:139578/1‑136 (MQ=255)      aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                             <  7:33244/136‑1 (MQ=255)      aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTcggcg                                                                                                                             >  2:96674/1‑136 (MQ=255)         cGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGggcggcg                                                                                                                          >  1:337843/1‑135 (MQ=255)           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTg                                                                                                                        >  2:50169/1‑136 (MQ=255)           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTg                                                                                                                        >  1:296928/1‑136 (MQ=255)           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTg                                                                                                                        >  5:149148/1‑136 (MQ=255)           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTGTGTTGGGGGGccgg                                                                                                                        >  6:287580/1‑134 (MQ=255)                  tCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCGGGGTGAGc                                                                                                                 >  5:276295/1‑136 (MQ=255)                      tACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGgc                                                                                                              <  8:41349/135‑1 (MQ=255)                           cATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGcac                                                                                                         <  3:122025/135‑1 (MQ=255)                             tCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTTGGCGGCCAGGTTAAGCGGcaccaccac                                                                                                      >  1:60133/1‑136 (MQ=255)                              cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGTTCAGCGGCAGCaccacc                                                                                                     >  2:239949/1‑136 (MQ=255)                               ggAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCCCCACCg                                                                                                    >  8:37269/1‑136 (MQ=255)                                gagaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGGGGGCGGGGTAATCGGCCGCcccaccc                                                                                                    >  4:148777/1‑134 (MQ=255)                                gagaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTTGTTAATCGGCAGCACCCCCgg                                                                                                   >  2:247493/1‑136 (MQ=255)                                   aaTACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTc                                                                                                <  8:38008/136‑1 (MQ=255)                                   aaTACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTc                                                                                                <  8:32815/136‑1 (MQ=255)                                        ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCg                                                                                           <  2:15570/136‑1 (MQ=255)                                            aGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAt                                                                                       <  1:19566/136‑1 (MQ=255)                                              gcgtcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtg                                                                                      <  1:50169/135‑1 (MQ=255)                                              gcggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGGTCCGGCGAGAtg                                                                                      <  3:219924/135‑1 (MQ=255)                                              gcggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCGGGGTCAGCGGCCGCACCCCCGGTTCCGGCGAGAtgc                                                                                     >  5:296819/1‑136 (MQ=255)                                                ggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtgct                                                                                    <  3:291820/135‑1 (MQ=255)                                                 gcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtgctgc                                                                                  <  5:223989/136‑1 (MQ=255)                                                  cTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtgctgc                                                                                  <  4:18552/135‑1 (MQ=255)                                                     aTGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGTGCGGGGGGGGTGAGCGGCCGCCCCCCCCGGTACGGCGGGGTGCTGCGGtg                                                                              >  5:14359/1‑135 (MQ=255)                                                     aTGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGGGGCCGGGTTGAGCGGGCGCCCCCCCGGGTGCGGGGCGATTCTGGTGTc                                                                              >  8:153053/1‑136 (MQ=255)                                                       gCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTcc                                                                             <  4:39217/135‑1 (MQ=255)                                                              gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTAGGCGGCCGGGTTGAGCGGCCGCCCCCCCGGGTGCGGCGAGAGTCTGCTGTCCGgggtggg                                                                     >  8:299347/1‑135 (MQ=255)                                                                   tCGGGGCGTGGCTACGTGGTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTGCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCAt                                                                  <  6:285332/134‑1 (MQ=255)                                                                      gTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGCCTGGTTGAGCGGCAGCACCACCGGGTCCGGCGAGGTGGTGCTGTGGGTGGTGGGCATCGcgt                                                             >  7:5833/1‑134 (MQ=255)                                                                      gTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGGTCCGGCGAGATTCTGCTGTCCGTGGTGGCCATCGCtt                                                             >  1:135521/1‑136 (MQ=255)                                                                       tGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGTCCTGGTTCAGCGGCAGCACCTCCTGTTCAGGCGAGATGCTGCTGTCCGTGGTTGACATCGCttt                                                            <  1:118161/136‑1 (MQ=255)                                                                       tGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCttt                                                            <  5:344346/136‑1 (MQ=255)                                                                       tGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCttt                                                            <  6:149148/136‑1 (MQ=255)                                                                        gCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCttt                                                            <  3:24849/135‑1 (MQ=255)                                                                             gCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTg                                                       <  8:289503/135‑1 (MQ=255)                                                                                aCGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTTTTCGGCGGGCTGGTTGAGCGGCCGCCCCACCGGGTCCGGCGAGATGCTGCTCGGCGGGGTGGCCCTGGCTTTTTTTtggg                                                    >  4:332984/1‑135 (MQ=255)                                                                                aCGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTGTAGGCGGCCTGGTTGAGCGGCCGCACCACCGGGTGCGGCGGGATTCTGGTGTGCGGGGTGGGCCTGGCGTTTATTtgggg                                                   >  5:281269/1‑135 (MQ=255)                                                                                 cGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGt                                                   <  8:176632/135‑1 (MQ=255)                                                                                    gtTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCGGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGAtc                                               >  2:300872/1‑136 (MQ=255)                                                                                    gtTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTAGGCGGCCTGGTTCAGCGGCAGGCCCACCGGATCCGGCGAGATGCTGCTGTCCGGGGTGGCGAGCGCGTTTCTTGGGCTGAta                                               >  6:170562/1‑135 (MQ=255)                                                                                       cGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGAtctt                                             <  2:246007/135‑1 (MQ=255)                                                                                                ccTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGc                                     <  7:299347/134‑1 (MQ=255)                                                                                                          ggTCTGTGCGGCAGCCTGGCCGCGTTTTTTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGGTCCCGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGGGATCTTCTCCCAGCGTCTGCAGcc                           >  8:287492/1‑134 (MQ=255)                                                                                                           gTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGGTGCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCCGCCGAg                        >  1:245515/1‑136 (MQ=255)                                                                                                           gTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGGTCCCGCGAGATGCTGCTGTCCGGGGGGGCCATCGCTTTTGTTGGGGTGATATTATCCCAGCGTCTGCACCCGac                        >  7:283171/1‑135 (MQ=255)                                                                                                                   ggCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATcggcg                <  1:319862/136‑1 (MQ=255)                                                                                                                    gCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATcggcg                <  4:358733/135‑1 (MQ=255)                                                                                                                     cAGCCTGGCCGCGTTTTGTTCGGCGTCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGTGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATcggcgg               <  3:295866/135‑1 (MQ=255)                                                                                                                       gCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCAc            <  2:366818/136‑1 (MQ=255)                                                                                                                         cTGGCCGCGGTTTTTTAGGCGGCCGGGTTGAGCGGCAGCACCACCGGTTCCGGGGTGGTGCTGCTGTACGTGGGGGGCCTCGCTGTTTTTTGGGGGATATTCTGCCACCGTCTGCACCCGCGCAGCCGGGgcgcgc          >  2:106371/1‑136 (MQ=255)                                                                                                                                 gTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGGCa  <  7:243632/136‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       ACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGGCA  >  ACB143/3138221‑3138484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑