Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,693,1830TG47.1% 4.6 / 14.5 51R238R (CGT→CGG) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (10/17);  new base G (4/20);  total (14/37)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGG‑AACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCAC  >  ACB143/5693051‑5693300
                                                                                                                                     |                                                                                                                     
aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGG‑AACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGgc                                                                                                                    >  5:169634/1‑136 (MQ=255)
     ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGG‑AACAGAGCGGCGTTCGTGCGCGCGTCAGCCACATCACCACCCCCCCTGGcgcgcg                                                                                                               >  1:20017/1‑136 (MQ=255)
       cgggCCAGCTTCCTCGGCCTCCGGGGATTCCCGTAGCCCGCGTAACCCGTGGCCATGGCGCGCACCCGGCTGGTGGGGC‑AACAGAGCGGCGGGCGCGGGCACTTCACCCACATCCCCCGCGCCCGTGGCGCGCGGc                                                                                                             <  6:262765/134‑1 (MQ=255)
           ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGG‑AACAGAGCGGCGTGCGTGCGCACGTCAGCCAGATCCCCAGCCCCCGTGGCGCGCGGCTg                                                                                                           >  4:245850/1‑134 (MQ=255)
              gcgtCCTCGCCCGGCCGGGCATCCGGTCTCGCGCCTAAAGCGTGCCCCGGGCGCGCAACCTGCTCGTGGGGC‑AACAGCGCGGCGGGCGTGCGCACTTCACCCACACCACCCGCGCCCGTGGCGCGCGGCTGATCg                                                                                                       <  4:281783/132‑1 (MQ=255)
               cTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGG‑AACAGAGCGGCGTGCGTGCGGCAGTCCGCCAGCTCACCAGCGCCCGGGGCGCGCGGCTGGACgcc                                                                                                     >  6:206727/1‑136 (MQ=255)
                ttCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGG‑CACAGAGCGGCGTGCGGGCGGCCTTGAGCCCGATCACCCGCGCCCGGGGCGCGCGGCTGCTCgccc                                                                                                    >  8:284792/1‑136 (MQ=255)
                  ccgcGGCCTGCCGGGCAGCCCGGCGAGCGCCTAAACGGTGGCCCTGGCGCGCACCCTGCTGCTGGTGC‑AACAGAGCGGCGGGCGTGCGCACTTCCGCCAGAGCACCCGCCCCCGTGGCGCGCGGCTGATCgcccag                                                                                                  <  8:120355/133‑1 (MQ=255)
                   cTCGGCCTGCCGGGCATCCCGTAGAGCGCCTAAACCGGGGCCCTGGCGCGCAACCTGCTGCTGGTGC‑AACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCgcccag                                                                                                  <  2:105415/135‑1 (MQ=255)
                         cggCCGGGCAGCCCGGTGAGCGCCGAACCCGTGGCCCTGGCGCGCAACGTGCGGTTGGGGG‑AACCGCGGGGCGTGCTGGCTCACTTCCGCCTGATCCCCTGCGCCGGTGGCGCGCGGCTGATCGcccaggcccagg                                                                                           <  2:20017/134‑1 (MQ=255)
                              gggCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCGGGTGG‑AACAGTGCGGCGGGCGTGCGCAGTTCACCCAGATCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                      <  6:368280/136‑1 (MQ=255)
                                         agcgcgcCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGG‑AACAGAGCGGCGTGGGGGGGGCCGTAAGCCCGCTCACCCGCGCCCCTGGGGCGCGGCGGGTAGCCCCCGCCCCGGGCGGGGGCCGGGCggg                                                                           >  1:243810/4‑135 (MQ=255)
                                            gcgcCTAACCGGGGGCCCTGGGGCGCAACCTGCGGCGGGGGC‑ACCCGCGGGGGGTGCGGGCGCACTTCCCCCAGAACCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                        <  5:218187/136‑1 (MQ=255)
                                            gcgcCGAAACGGGGGCCCTGGCGCGCAACCGGCGGCGGGGGG‑AACCGAGCGGCGGGGGGGCGCACTTCCGCCAGAGCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                        <  8:45024/136‑1 (MQ=255)
                                            gcgcCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGG‑AACAGAGCGGCGTGGGTGGGCCCATCAGCCACATCACCCCCGCCCGGGGCGCGCGGCGGATCGCCCCGGCCCCCGGCGTGGGGCGGCCGGTAAc                                                                        >  1:60067/1‑136 (MQ=255)
                                             cgcCGAAACGGGGGCCCTGGCGCGCAACCTGCTGGTGGTGC‑AACAGCGCGGCGTGCGTGCGCACTTCAGCCAGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                        <  4:126089/135‑1 (MQ=255)
                                                cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGG‑AACAGAGCGGCGTGCGTGCGCCCATAAGCCAGATCACCCGCCCCCGTGGCGCGCGGCGGATAGCCCCGGCCCCGGCCGTGGGCCTGCCGGTGAccgc                                                                     >  3:259999/1‑135 (MQ=255)
                                                      gggggCCCTGGCGCGCAACCGGCTGGGGGTGT‑AACCGAGCGGCGTGCGTGCGCACTTCCGCCATAACACCTGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                              <  7:157594/133‑1 (MQ=255)
                                                      ggTGGCCCGGGCGCGCACCCTGCTGCTGTGGG‑AACAGAGCGGCGTGCGTGGGCACTTCCGCCACATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                              <  7:278209/135‑1 (MQ=255)
                                                      cgtggccgtggcGCGCAACCTGCTGCTGGTGG‑AACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                              <  6:158536/136‑1 (MQ=255)
                                                      cggggCCGTGGCGCGCAACCTGCGGCGGGTGC‑AACTGAGCGGCGTGCGTGCGCAATACACCCAGAACACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                              <  1:205803/133‑1 (MQ=255)
                                                      cGTGGCCCTGGCGCGCAACATGCTGGTGGGGG‑AACAGAGCGGGGGGCGTGCGCACTTCACCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                              <  2:209798/136‑1 (MQ=255)
                                                       gTGGCCCTGGCGCGCAACCTGCTGCGGGTGG‑AACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCCCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                              <  4:122519/135‑1 (MQ=255)
                                                             cTGGCGCGCAACCTGCTGCTGGTGG‑AACAGAGCGGCGTGCGTGCGCACATCAGCCAGATCCCCCCCCCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCTGGGGCCGGGCGGTTACCGCCGCGGTGGCGCt                                                         >  4:303779/1‑134 (MQ=255)
                                                              tGGCGCGCAACTTGATGC‑GGTGGTAACTTAGGGGGGTGCTTTGGCACTACAGCCAGATCCCCAGCCCCGGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGt                                                       <  4:181198/135‑1 (MQ=255)
                                                                gcgcgcAACGTGCGGCTGGTGG‑AACAGAGCGGCGGGCGGGCGCACTTCAGCCTGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTAc                                                     <  8:302612/135‑1 (MQ=255)
                                                                gcgcgcAACCTGCTGCTGGTGG‑AACAGAGCGGCGTGCGGGCGCACTGCAGCCACACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTAc                                                     <  3:59387/135‑1 (MQ=255)
                                                                  gcgcAACCTGCTGCTGGTGG‑AACAGAGCGGCGTGGGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCGGATCGCCCCGGCCCCGGCCGTGGGCCTGCCGGGAACCGCCGATGTAGCGCTGGTCCAg                                                  >  2:282381/1‑136 (MQ=255)
                                                                   cgcAACCTGCGGCTGGTGT‑AACTCAGCGGGGTGCGGGCGCACTTCCCCCACACCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg                                                  <  4:49100/135‑1 (MQ=255)
                                                                                     g‑AACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATAACCCCCGCCCGGGGCGCGCCGCGGCGAGCCCACGCCCCGGGCGTGGGCCAGCCGGTAACCCCCGAAGTAGCGCAGTACCAGCGGATCCGCCCggccccg                                >  4:200397/1‑130 (MQ=255)
                                                                                             gcggcgTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                        <  7:84715/136‑1 (MQ=255)
                                                                                             gcggcgGGCGTGCGCACTGCACCCCGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                        <  7:8128/136‑1 (MQ=255)
                                                                                              gggcgggcgggcgAACTTCAGCCACAGCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                        <  3:72912/134‑1 (MQ=255)
                                                                                              cggcgTGCGTGCGCACTTCACCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                       <  2:62910/136‑1 (MQ=255)
                                                                                              cggcgTGCGGGCGCACTTCCGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                        <  3:288716/135‑1 (MQ=255)
                                                                                                gcgtgcgtGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCGTGGGCCTGCCGGTAACCGCCGATGTAGCGCGGTACCAGCTGATCCGCACGGCCGAGTCGCGgcgc                       >  8:84715/1‑134 (MQ=255)
                                                                                                gcgtgcgtGCGCACTTCAGCCAGAGCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGa                     <  1:286925/136‑1 (MQ=255)
                                                                                                  gtgcgtggGAAGTTCTGCCTGATCACCTGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                   <  7:54081/136‑1 (MQ=255)
                                                                                                  gggggggcgcACTCCACCCACACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                   <  5:33148/130‑1 (MQ=255)
                                                                                                  gggcgtgcgCACTTCAGCCAGACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                   <  2:239073/134‑1 (MQ=255)
                                                                                                  gggcgggcgCACTGCCCCCAGATCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                   <  6:275578/134‑1 (MQ=255)
                                                                                                  gggcgggcgCACTGCAGCCAGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                   <  6:117289/134‑1 (MQ=255)
                                                                                                   tgcgtgcgAACTTCCGCCACATCACCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt                  <  7:94246/136‑1 (MQ=255)
                                                                                                   ggggtgggCACTACAGCCACTGCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt                  <  8:120476/133‑1 (MQ=255)
                                                                                                   ggcgggcgCACTGCCGCCACATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                   <  4:315423/134‑1 (MQ=255)
                                                                                                   ggcggccgcACTTCCGCCCGAACCCCCCCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                   <  4:153354/129‑1 (MQ=255)
                                                                                                       ggcgaACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc              <  8:88783/135‑1 (MQ=255)
                                                                                                        gcccACTGCAGCCACAGCACCTGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCa             <  1:267012/133‑1 (MQ=255)
                                                                                                                cAGCCCGACCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTa      <  2:15692/135‑1 (MQ=255)
                                                                                                                 aGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTAcc    >  5:272328/1‑136 (MQ=255)
                                                                                                                   ccAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCAc  >  8:224891/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                     
AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGG‑AACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCAC  >  ACB143/5693051‑5693300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑