Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1436,043,1960CA36.9% 40.4 / 31.0 65A37S (GCC→TCC) puuD_3Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base A (0/24);  total (18/47)
Fisher's exact test for biased strand distribution p-value = 8.34e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAC  >  ACB143/6043065‑6043327
                                                                                                                                    |                                                                                                                                   
gggggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAg                                                                                                                                 >  1:65612/1‑136 (MQ=255)
gggggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAg                                                                                                                                 >  7:182470/1‑136 (MQ=255)
  gggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAgc                                                                                                                                >  3:194963/1‑135 (MQ=255)
   ggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAgccg                                                                                                                              >  5:250319/1‑136 (MQ=255)
    gcgcCATTTTAATGATGGGCGTTTTTATTTGACGGCGCGCGGTAAAAAACATTGCGGGGCTGGCGAGCCAGCCGCGCTTGCGTGTCGCTGCCGCCATCCCGGGCCGGC‑AAGATCCGTGCCCAGCCGGAAAAgccgc                                                                                                                             <  2:33604/136‑2 (MQ=255)
    gcgcCATTGTAATGATGCGGCTCGATGTTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCACGCCGGCAACgccgg                                                                                                                             >  7:160821/1‑136 (MQ=255)
    gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAgccgg                                                                                                                             >  6:189374/1‑136 (MQ=255)
     cgcCATTGTAATGATGCGGCTCGATATTTGACGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAACCCGGAAAAgccggc                                                                                                                            <  2:225466/136‑1 (MQ=255)
     cgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGGGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAgccggc                                                                                                                            <  7:55806/136‑1 (MQ=255)
         aTTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGgccgcc                                                                                                                        >  5:113812/1‑136 (MQ=255)
                     cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACAGACtt                                                                                                             >  4:308249/1‑135 (MQ=255)
                       gCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGt                                                                                                           >  3:322410/1‑135 (MQ=255)
                       gCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTc                                                                                                          >  6:30692/1‑136 (MQ=255)
                          cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGcc                                                                                                       >  1:163593/1‑136 (MQ=255)
                           gATATTTGACGGCGAGCCGGTAAAAACAATGCCGTGCAGGGGAGCCAGCTGCGCTTGCGTGTCGCTGCCGCCAACACGGGCCGGC‑AAGATCTGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGcc                                                                                                       <  7:281309/135‑1 (MQ=255)
                                    aGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGc                                                                                             <  1:301122/136‑1 (MQ=255)
                                       cGAGCCGGTAAAAACAATGCCGGGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgag                                                                                          <  1:87136/136‑1 (MQ=255)
                                       cGAGCCGGTAAAAAAACTGCCGGGCTGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCACCACGGGCCGGA‑AAGATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgag                                                                                          <  8:4455/136‑1 (MQ=255)
                                              gTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGttct                                                                                   >  8:226890/1‑136 (MQ=255)
                                                               cAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg                                                                   <  1:113555/135‑1 (MQ=255)
                                                                tggCGAGCCAGCAGCGCTGGCGTGGCGCGGCCGCCATCCGGGGCCGCA‑AATATCAGGGGCACGCCGCAAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg                                                                   <  3:121067/133‑1 (MQ=255)
                                                                aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCGCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACgg                                                                  >  6:317094/1‑135 (MQ=255)
                                                                     agacagcagCGCTTGCGTGTCGCTGCAGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGgcg                                                            >  2:290147/4‑136 (MQ=255)
                                                                         agcagcGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATTCTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa                                                        <  6:327591/136‑1 (MQ=255)
                                                                         agcagcGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGA‑AAGATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa                                                        <  6:182309/136‑1 (MQ=255)
                                                                                         cGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACATGTCGCCGCCCGTGTGCAAGGAGGTCTTCCCTACCTGCTGACGGCAGGCGCTGCCAACGATAAAAGGGAc                                        >  8:331318/1‑136 (MQ=255)
                                                                                              ccgccACCACGGGCCGGC‑AAGATCAGGGGCAACCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt                                   <  5:263525/136‑1 (MQ=255)
                                                                                              ccgcaATCACGGGCCGCA‑AAGTTCAGTGGCACGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt                                   <  7:29447/136‑1 (MQ=255)
                                                                                               cgcaATCAGGGGCGGCC‑AAGAGCAGGGCCACGCCGGAAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt                                   <  4:246577/135‑1 (MQ=255)
                                                                                                    tccggggCGGTC‑AAGATCTTGGGCAAGCGGGCAAAGCCGGCCGCCTCGACATACTTGGCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  8:334305/132‑1 (MQ=255)
                                                                                                    tCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  7:38329/136‑1 (MQ=255)
                                                                                                    tCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  6:113812/136‑1 (MQ=255)
                                                                                                    tCACGGGCCGGC‑AAGATCAGTGCCAACCCGGCAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  5:276655/136‑1 (MQ=255)
                                                                                                    tCACGGGCCGGA‑AAGATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  7:2484/136‑1 (MQ=255)
                                                                                                    tCACGGGCCGCA‑AAGTCCTGTGGCAACCCGCCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  2:221737/136‑1 (MQ=255)
                                                                                                    tCACGGCCCGGC‑AAGATCATTGCAAACCCGGAAAACCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  2:252465/136‑1 (MQ=255)
                                                                                                    gcccGGGCCGTC‑AAGATCTGGGCCACGCCGGAATAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  5:369645/133‑1 (MQ=255)
                                                                                                    gcaggggCGGGA‑AATATCAGTGCAACGCCGGAAACGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  1:75394/132‑1 (MQ=255)
                                                                                                    cccggggCCGGC‑AAGATCAGTGGAAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  1:184309/132‑1 (MQ=255)
                                                                                                    ccccGGGCCGGC‑AAGACCTGTGCCACGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  1:335583/133‑1 (MQ=255)
                                                                                                    acaggggCCGCC‑AAGATCCGGGGCAACCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  7:20748/132‑1 (MQ=255)
                                                                                                     tccGGGGCGGCAAATATCAGTGGAAAGCCGGAAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  5:17019/134‑1 (MQ=255)
                                                                                                     cccgggcgggA‑AAGATCAGTCACACCCCGCAAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  4:132020/133‑1 (MQ=255)
                                                                                                     caggggCGGGA‑AAGATCTGGGGCACGCCGGAAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  4:322410/132‑1 (MQ=255)
                                                                                                     caggggCCGGC‑ATTTTCTTTGGAAAGCGGGAAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  3:60458/132‑1 (MQ=255)
                                                                                                     cACGGGCGGGA‑ATGATCTGTGGCAAGCGGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  3:201468/135‑1 (MQ=255)
                                                                                                     cACGGGCCGGC‑ATGATCAGGGGCAAGCGGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  4:200547/135‑1 (MQ=255)
                                                                                                     cACGGGCCGGC‑AAGATCTGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  4:84615/135‑1 (MQ=255)
                                                                                                      aCGGGCCGGC‑AATATCAGTGGCACGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  5:89523/134‑1 (MQ=255)
                                                                                                       cGGGCCGCC‑CAGATCAGTGCCAAGCCGGCAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  4:340256/133‑1 (MQ=255)
                                                                                                        gggCCGGG‑AATATCAGTGGCAAGGCGGCAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  7:18074/132‑1 (MQ=255)
                                                                                                           ccGGC‑AAGATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATggg                      <  2:251336/136‑1 (MQ=255)
                                                                                                           ccGGC‑AACATCAGGGGCAACCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATggg                      <  2:291609/136‑1 (MQ=255)
                                                                                                             ggC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGc                     >  4:40662/1‑135 (MQ=255)
                                                                                                                            cAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAgg                                           <  1:214408/99‑1 (MQ=255)
                                                                                                                            cAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAgg                                           >  2:214408/1‑99 (MQ=255)
                                                                                                                            cAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAg      >  6:16860/1‑136 (MQ=255)
                                                                                                                             accccGGAAACGCGGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg     <  1:351873/133‑1 (MQ=255)
                                                                                                                             aaGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg     <  2:329009/136‑1 (MQ=255)
                                                                                                                             aaGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg     <  7:95378/136‑1 (MQ=255)
                                                                                                                             aaGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg     <  2:74817/136‑1 (MQ=255)
                                                                                                                             aaGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg     <  2:84258/136‑1 (MQ=255)
                                                                                                                                ccGGCAATGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAc  <  1:321490/136‑1 (MQ=255)
                                                                                                                                ccGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCACAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAc  <  5:262279/136‑1 (MQ=255)
                                                                                                                                 cGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAc  <  3:89438/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
GGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAC  >  ACB143/6043065‑6043327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑