| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 1,123,238 | C→A | intergenic (‑240/+64) | gcvH_1 ← / ← CJ019_01062 | Glycine cleavage system H protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,123,238 | 0 | C | A | 80.8% | 19.9 / ‑4.9 | 28 | intergenic (‑240/+64) | gcvH_1/CJ019_01062 | Glycine cleavage system H protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (4/1); new base A (21/0); total (27/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGA > ACB143/1123113‑1123369 | aCGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGGGacgacg > 3:21912/1‑135 (MQ=255) agaAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAACGATa > 7:359324/1‑135 (MQ=255) gtgGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTAAGACGGAGCGAAAACGATACATTGGGTGGCTGGACTgg > 2:100687/1‑136 (MQ=255) gtgGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGCCGTAGCGCAAACGATACATGTGGTGCCGGGTCTgg > 8:182328/1‑136 (MQ=255) gtgGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAACGCTACAAGTGGTGCCGGGGCTgg > 6:357612/1‑136 (MQ=255) ggAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAACGATACAAGTGGTGCCTGGTCTGGCg > 1:240067/1‑136 (MQ=255) aaTGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTTGCGCAAACGATTCATGTGGTGGCGGGCCTGGCGAg > 5:306472/1‑136 (MQ=255) gATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTAAGACCTAGCGAAAAAGCTACATGTGGTGCCGTGCCTGGCGAGGTg > 8:210555/1‑136 (MQ=255) ttACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACCACGTAGCGAAAACCAAACATTTGGTGCCTGGACTGGCGAGATGCCAATgg > 2:313442/1‑136 (MQ=255) cAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGGGACGACGGAGCGAAAACGATACATGTGGTGGCGGGACTGGCGAGGTTGCAATGGccg > 5:195098/1‑135 (MQ=255) cAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGCCGGTGCGTAAACGATACATTTGGTTCCTGGGCTGGCGGGGTGCCAATGGcc > 3:45939/1‑135 (MQ=255) cAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGCCGGTGCGAAAAAGCAAAAAGTGGTTGCGGGGCGGGGGCGGTGGCAATGGccg > 7:322734/1‑135 (MQ=255) cAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTAAGACGTAGCGCAAACGATACATGTGGTGCCTGGTCTGGCGAGGTGCCCATTGccg > 6:350434/1‑135 (MQ=255) cAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTAAGACGTAGCGAAAACGATACATGTGGTGCCTGGCCTGGCGAGGTGCCAATGGCCt > 7:271820/1‑136 (MQ=255) ggTGCCTCTGAGAGGAAACGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAACGATACATGTGGTGGCTGGACTTGCGAGGTGCCAATTGCCGTc > 1:332718/1‑136 (MQ=255) ctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGCCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtc > 8:360199/1‑136 (MQ=255) ctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGGTTTAGCGTCGCCGGAGCGCAAACGATAAAAGTGGTGGCGGGGCGGGGGGGGTGGCAATTGCCGTGGGtga > 4:162509/1‑132 (MQ=255) gagaGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTTAGACGGAGCGCAAACGATACATTTGGTGCCTGGACTGGCGCGGTGGCAATGGCCTTCgggaatgt > 5:371688/1‑129 (MQ=255) aaGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGTGACATGTGGTGGCGGGGCTGGCGCGGTGCCAATGGCCTTCGGTGATGTCCCGTTcag > 2:95059/1‑136 (MQ=255) aGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCGTCGACGGTGGGCAAACGATACATGTGGTGGCGGGGCTTGCGGGGTGCCAATTGCCGTCGGTCCTCTCACTTTgag > 8:211838/1‑135 (MQ=255) aGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTACCAACCCCGGAACGAAAACGCTACAGGGGGGGGCAGGGCAGGCGAGGTGCCAAAGGCCTTAGGTACTCACACTTTcac > 4:304424/1‑134 (MQ=255) gggTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTTGCGACGACGTTGCGCAAACGAAACATGTGGTGGCTGGGCTGGCGAGGGGGCAATGGGCCTAGGTGCTCTCACGTTcagc > 4:374535/1‑135 (MQ=255) gCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAACGATTCATGTGGTGGCTGGACTGGGGAGGTGCCAATGGGCTTCGGTGATCTCACTTTGAGCAGATGATc > 8:251988/1‑135 (MQ=255) cATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGGAAACGATACATGTGGTGCCTGGACTGGCGAGGTTCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCa > 1:296086/1‑136 (MQ=255) gCACCGACGGCGAGGAGGCTGTGTATCGTTTTTTGTAAGACGTTGCGAAAAAGAGAAATGTTGTGGCAGGACCTGCGAGGGGGCCATGGCCTTAGGTCATATCACATTCAGCAGATTATCCATCGAAAACGccccc > 2:233228/1‑135 (MQ=255) cACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGCCGTATCGAAAAACATACAAGTGGTGCCTGGACTGGCGAGGTGCCAAAGGCCTTCGGTCATCTCACTTTAAGCAGATGATCAACCGAAAAACCGCCgg > 8:360899/1‑136 (MQ=255) cGGCGAGGAGGCTGTGTATCGTTTTAGCGAACACGTAGCGCAAACCAAAAATGTGGTTGCTGGGCTGGGGCGGTGCCAATTGCCGTAGGTGATCTCACATTAAGCAGATGATCCAGCGAAAACGCCCCGGCGCCTc > 2:9605/1‑136 (MQ=255) tttCGATACGACTTAGCTAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAaga < 7:251988/135‑1 (MQ=255) | ACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGA > ACB143/1123113‑1123369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |