| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,386,510 | 2 bp→TA | intergenic (‑519/+1131) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,510 | 0 | A | T | 96.2% | 62.8 / ‑6.6 | 27 | intergenic (‑519/+1132) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (11/14); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | ACB143 | 5,386,511 | 0 | T | A | 96.2% | 77.5 / ‑4.3 | 27 | intergenic (‑520/+1131) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base A (11/14); minor base C (1/0); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑CGT‑‑‑CA‑GGAT‑C‑‑A‑‑T‑CCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGG > ACB143/5386410‑5386604 || ccTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGc > 1:140318/1‑136 (MQ=255)ccTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGc > 8:196722/1‑136 (MQ=255) ttGATAACAAAAATTGATCTAATTCTTTAATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGGCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGaa < 1:381239/135‑1 (MQ=255) aCAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCAc > 4:93987/1‑135 (MQ=255) ttGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg < 4:38452/135‑1 (MQ=255) tCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt < 8:284278/134‑1 (MQ=255) tCATTAAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGGT‑‑‑TA‑CGCTAC‑‑AACC‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt < 6:40934/134‑1 (MQ=255) aTTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCGCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 7:90429/136‑1 (MQ=255) aTTTAACACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑C‑‑AACC‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 7:36010/136‑1 (MQ=255) aCCCTAATATGACATCTCGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt < 1:155497/136‑1 (MQ=255) ccTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGCGGGCTTTTTTATTGCGCTGCGGGTGc > 6:291024/1‑136 (MQ=255) ttatGACATCGCGGCCGGCTGGTCGACGACGGGGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 2:383321/135‑1 (MQ=255) atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 6:139809/136‑1 (MQ=255) atatGACATCTCGGCCGGCGTGTCGACGACGGCGGTCGCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGAGGTTGCCAgg < 8:255959/136‑1 (MQ=255) atatCACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 1:143764/136‑1 (MQ=255) tatGACATCTCGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 4:337739/135‑1 (MQ=255) ctcGGCCGGCTTGTTGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCGTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 5:3657/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 5:69561/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 6:362927/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 1:216374/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTATTATTGCGCTGCGGGAGCCAGGGCGTGccc > 3:212377/1‑135 (MQ=255) tcGGCCGGCTTGTCGACCACGGCGGTCTCAGT‑GGTGTACG‑GTAC‑A‑‑A‑‑TCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGATGCGGGTGCCAGGGCGTGACCtt > 5:313863/1‑136 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg > 5:132480/1‑136 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg > 7:312245/1‑136 (MQ=255) gccggcTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt < 4:348427/133‑1 (MQ=255) gccggcTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt < 4:192186/133‑1 (MQ=255) gccggcTTGTCGACGACGGCGGGCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt < 4:183160/133‑1 (MQ=255) || CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑CGT‑‑‑CA‑GGAT‑C‑‑A‑‑T‑CCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGG > ACB143/5386410‑5386604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |