Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	ACB143	4,257,335	+GCGGCCGC	intergenic (‑281/+7)	CJ019_03991 ← / ← CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	ACB143	= 4257334	0 (0.000)	46 (0.800) +CGCGGCCG	30/242	0.5	100%	intergenic (‑280/+8)	CJ019_03991/CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein
	?	ACB143	4257335 =	0 (0.000)					intergenic (‑281/+7)	CJ019_03991/CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein

GGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/4257199‑4257334 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTGGC  >  ACB143/4257335‑4257463                                                                                                                                         ||||||||                                                                                                                                  GGCGCCCCATCAATGTATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATTGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCC                                                                                                                                          <  4:62680/137‑1  GCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCG                                                                                                                                         >  1:62794/1‑137     CCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTAGCGAACAAAATGCATTGACCTACCACGCCGACCGCATCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGG                                                                                                                                      <  3:273966/137‑1       CCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTTATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    <  7:70701/137‑1       CCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    <  1:306971/137‑1       CCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    <  2:77513/137‑1       CCATCAATGGATCCACTAAAAACCGATAAACGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    <  6:35063/137‑1       CCATCAAGGCATCCCCAAAACACCGATAACCTCAAAGAATCCGCACCTCCCGAACAAACTGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                    <  4:84837/137‑1        CATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCG                                                                                                                                   <  1:236477/137‑1        CATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAAT‑AAAACAAAACCCGGAGAGCCTCCCGCGGCCGC                                                                                                                                  <  3:92523/137‑1         ATCAATGGATCCACTAAAAACCGAAAATCGCACACAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCTACCGCAGCCTTAATGGATCCATAAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGC                                                                                                                                  <  2:359476/137‑1                  TCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTA                                                                                                                         <  1:257868/137‑1                   CCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTAC                                                                                                                        <  6:107296/137‑1                      aTAAAAACCGAAAATCGCAAAGAATCCGCACCAACCGAAAAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGAC                                                                                                                     <  1:317228/136‑1                      CTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGAC                                                                                                                     >  8:338138/1‑137                                   ATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGA                                                                                                        >  6:184712/1‑137                                   ATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGA                                                                                                        >  5:325315/1‑137                                    TCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGTAGAGCCTCCCGCGGCCGCCCCGTCTTAgg                                                                                                                       <  4:34708/121‑3                                    TCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACG                                                                                                                       >  3:34708/1‑121                                           cAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATG                                                                                                <  4:41882/136‑1                                               CCGCACCTACCGAACAAAATGCATTCACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCG                                                                                            <  5:8617/137‑1                                                         CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  <  2:62794/137‑1                                                         CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  >  4:178460/1‑137                                                         CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  >  4:226151/1‑137                                                         CGAACAAAATGAATTGAAATACAACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAACCCCGGAGAGCCTCCCGCGGCCGCCCCGTGTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  >  7:156402/1‑137                                                          cAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCA                                                                                 <  8:4329/136‑1                                                          GAACAAAATGCATAGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCc                                                                                 >  2:97925/1‑136                                                              AAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGCCTTTCATGCCCGCGTCTGCCGCAGCCC                                                                             >  5:52116/1‑137                                                                  TGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGACCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCC                                                                         <  2:210959/137‑1                                                                       TGACCTACCACCCCGACCTCAGCCTTACTGGATCCATTAAATAAATACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGTCTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGG                                                                    <  8:136824/137‑1                                                                            aACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCG                                                               <  4:59000/136‑1                                                                            aACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCG                                                               <  5:212218/136‑1                                                                            TACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCG                                                               <  3:178460/137‑1                                                                            TACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCG                                                               <  5:373682/137‑1                                                                            TACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCG                                                               <  5:136730/137‑1                                                                                 CGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATA                                                          <  6:325315/137‑1                                                                                      ACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCA                                                     >  5:238927/1‑137                                                                                        CGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAG                                                   >  1:325256/1‑137                                                                                         GCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCCGCCCCGCCAAAGGTTTCGGCATAGCGCAAGG                                                  >  8:274348/1‑137                                                                                         GCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGG                                                  >  4:80753/1‑137                                                                                         GCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGG                                                  >  4:183301/1‑137                                                                                              CTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCA                                             <  4:337110/137‑1                                                                                                TAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATT                                           <  8:163193/137‑1                                                                                                    GGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCG                                       >  8:351337/1‑137                                                                                                     GATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGT                                      >  5:297846/1‑137                                                                                                         aATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTC                                  <  5:212413/136‑1                                                                                                          ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCG                                 >  5:244188/1‑137                                                                                                          ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCG                                 >  3:269591/1‑137                                                                                                          ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCCGCATAGCGCAAGGTGGCATTGGCGTGCTCG                                 >  3:350735/1‑137                                                                                                          ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  >  7:33881/1‑88                                                                                                          ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                  <  8:33881/88‑1                                                                                                               ATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCAT                            <  6:139040/137‑1                                                                                                                    AACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCG                       >  5:209388/1‑137                                                                                                                       AAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGAAGGATGACTTTCATGCCAGCGTCTGCAGCAGCCACGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTTCTAGCGCATGATCGCTT                    >  5:280674/1‑137                                                                                                                                         CGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCCTCTGCCGCAGCACCGCCAAAGGTTTCGGAATAGCGCAAGGTGGCATTGGCGTGCTGGCGCATGATCGCTTCGGCGCGGGCACACTGGC  >  2:59445/1‑137                                                                                                                                         ||||||||                                                                                                                                  GGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/4257199‑4257334 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTGGC  >  ACB143/4257335‑4257463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.