Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,653,7460CG30.6% 64.4 / 10.6 36S10T (AGC→ACC) mtnB_2Methylthioribulose‑1‑phosphate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (2/23);  new base G (11/0);  total (13/23)
Fisher's exact test for biased strand distribution p-value = 1.30e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCACGCGGACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGG  >  ACB143/3653615‑3653882
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gCACGCGGACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCtggtg                                                                                                                                     <  3:69278/137‑1 (MQ=255)
ccACGCGGACCGAGAAGTGCGGGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCtggtg                                                                                                                                     <  7:185220/136‑1 (MQ=255)
      ggACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAg                                                                                                                               <  1:190935/137‑1 (MQ=255)
       gACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAgg                                                                                                                              <  8:376146/137‑1 (MQ=255)
       gACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAgg                                                                                                                              <  1:56918/137‑1 (MQ=255)
        aCCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGc                                                                                                                             <  2:341662/137‑1 (MQ=255)
            ataaGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGAc                                                                                                                         <  3:167112/135‑1 (MQ=255)
             gaAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGGCGTTGGAGGTGGTGGGGGTGAAGGCAGACa                                                                                                                        >  3:328205/1‑137 (MQ=255)
                      gtAGATTTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCa                                                                                                               <  8:163241/137‑1 (MQ=255)
                          aTGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATggg                                                                                                           <  8:248784/137‑1 (MQ=255)
                          aTGGGGGCGGTCCAGCGCAGCAGCGCAGACAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATggg                                                                                                           <  4:341051/137‑1 (MQ=255)
                              ggtcggtcCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTGGTTTGAGGGGGGGGTGGTGGAGGGGGGCAGGGTACTCAAGGGGctg                                                                                                       >  3:111305/1‑136 (MQ=255)
                                  ggtcCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCg                                                                                                   <  5:148870/137‑1 (MQ=255)
                                  ggtcCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGGGGTGTCGGGGGGGGGTGGTGGAGGCGGACAGTGAAATGCTTGGGCTCTCCg                                                                                                   >  3:70918/1‑137 (MQ=255)
                                  ggtcCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGGCGTTGGTGGGGGGGGGGGGGTAGGGAGCCAGTGAGCTCATGGGGCTCGCCg                                                                                                   >  1:54639/1‑137 (MQ=255)
                                       agcgcagcagcgcagcCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTCGGTGGAGGTGCTGGGGGTGAAGGCAGACAGTGAACGCCTGGGGCGCTCCgggtat                                                                                              >  5:340400/1‑133 (MQ=255)
                                                gcgcagcCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTCGGTGGCGGGGGTGGGGGTGGAGGGGGACAGTGTACTGATTGGGCTCTCCGGGTATAAATATccc                                                                                     >  5:282780/1‑136 (MQ=255)
                                                 cgcagcCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGGCGGTGGCGGGGGGGGGGGGGGTGGCGGACACGGAACACATGGGGCTCTCCGGGAATCAAAAgaccc                                                                                    >  3:164834/1‑133 (MQ=255)
                                                 cgcagcCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGACGTTGGGGGGGGGGGTGGTGTAGGCAGCCAGTGATCTCATGGGAGTCTCCGGGAATAAATATCcgc                                                                                    >  5:140320/1‑137 (MQ=255)
                                                         gACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTCGTTTGGGGTGGGGGGGGGGTGGGGGGCCACTGAACTTATGGGGCACGGCGGGTATCAAAAGCCGCGCCCCggg                                                                            >  2:306634/1‑135 (MQ=255)
                                                          aCGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCgtgt                                                                           <  1:247752/137‑1 (MQ=255)
                                                               aaGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGAcc                                                                      <  2:31733/137‑1 (MQ=255)
                                                               aaGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGGCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGAcc                                                                      <  4:96545/137‑1 (MQ=255)
                                                                     cggcCAGCGTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGGCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTg                                                                <  1:163381/137‑1 (MQ=255)
                                                                          aGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTCGGTGGAGGGGGGGGGGGGGTAGGCAGACAGTGAACTCATGGGGGTCGCCGGGAATAAAAAGCCGCGCGCCGGGTCGCCCAGGGTGGCCac                                                           >  4:131801/1‑136 (MQ=255)
                                                                                        cTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTcgc                                             <  6:140320/137‑1 (MQ=255)
                                                                                        cTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTcgc                                             <  4:170196/137‑1 (MQ=255)
                                                                                        cTCCCACTCTTCGGCGCTGACCCGGTCGCGGGGGGTTGGGGGGGGGGGGGGGTAGGGAGACAATGGACGAAAGGGTCTCTGCGGGTATAAATATCCCCGCGCCGGGTCGACCACGGTGGGGAGGGGGGCCTGGTcgg                                             >  6:320257/1‑136 (MQ=255)
                                                                                              ctctTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGTCTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGc                                       <  6:173492/137‑1 (MQ=255)
                                                                                               tctTCGGCGCTGACCCGGTCGCGGTCGGGGGTGGGGGGGGTGGGGGAGACACACAGGGCACTCATGGGGCTCTGCCGGAATAAATAGCCCCCCGCCCGGTCGCCCCCGGTGGGCAGGGGGGCCCGCGCGCgggggcg                                      >  4:259941/1‑132 (MQ=255)
                                                                                                    ggCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGa                                 <  6:204893/137‑1 (MQ=255)
                                                                                                            cccGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCg                         <  2:233593/137‑1 (MQ=255)
                                                                                                              cGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGAt                       <  4:164834/137‑1 (MQ=255)
                                                                                                                        cGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCg             <  4:315927/137‑1 (MQ=255)
                                                                                                                          ttGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCgag           <  4:328205/137‑1 (MQ=255)
                                                                                                                              aGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAg       >  5:111421/1‑137 (MQ=255)
                                                                                                                                   cTGGTGGTGAAGGCAGACAGTGACCTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCgg  <  5:96138/137‑1 (MQ=255)
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GCACGCGGACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGG  >  ACB143/3653615‑3653882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑