Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,277,0290CG30.0% 61.6 / 10.1 45F279L (TTC→TTG) hbd3‑hydroxybutyryl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (10/18);  new base G (12/0);  total (27/18)
Fisher's exact test for biased strand distribution p-value = 1.19e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGATCGGCCTGGACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGC  >  ACB143/4276893‑4277156
                                                                                                                                        |                                                                                                                                 
tGATCGGCCTGGACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTttc                                                                                                                                   >  8:18306/1‑137 (MQ=255)
     ggCCTGGACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCAc                                                                                                                              <  3:279179/137‑1 (MQ=255)
                cgtgctgcCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGAAAATACCGCCCTGCTCCACTGCTCAAGTAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTgaggag                                                                                                                   <  6:270153/135‑1 (MQ=255)
                   gctgGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGACTGGCTGGGGCGCAAGAGCGGTCGCGGTTTATTGAACGACTGAGGAGCaa                                                                                                                >  8:367990/1‑137 (MQ=255)
                           tcttGGAGGCCTTCCACGAAGGCTTCAACGACAGAAAATACCGCCCTGCTCCAGTGCTCAAGTAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATg                                                                                                        <  6:355464/134‑1 (MQ=255)
                             aTGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTAACCGAATGTGGAGCAAGCGCCATGcc                                                                                                      >  5:211860/1‑137 (MQ=255)
                                   cccTACCACCCAGGCTTCAACGACACCAAATCCCGCCCTGCGCCACTCCGCAAGGAAATGGCCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGt                                                                                                <  8:140679/136‑1 (MQ=255)
                                      ttCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTTGTAACCGACTGGGGTGCAAGCGCCATTCCCCCCGGCaa                                                                                             >  3:351754/1‑137 (MQ=255)
                                          aCGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCTTCTGGGGAGCAAGCGCCAATCCCCCCGTCAATGcc                                                                                         >  8:146666/1‑137 (MQ=255)
                                          aCGAAGGCTGCAACGACAGAAAATCCCGCCCTGCTCCACTGCTCAAGGAACTGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGcc                                                                                         <  3:130372/137‑1 (MQ=255)
                                           cgcaGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCGTAACCAACTGAGGCGCCAGACCCCTGCCCCCCGGCAATGccc                                                                                        >  5:332785/4‑136 (MQ=255)
                                               ggCTTCAACGACAGCAAATACCCCCCTGCTCCACTCCTCAAGCAACTGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAAt                                                                                    <  6:208590/137‑1 (MQ=255)
                                                gCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATg                                                                                   <  1:8887/137‑1 (MQ=255)
                                                 cTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCGACTGAGGAGCAAGCGCCCAGCCCCCGGGCAAAGCCGCAATgc                                                                                  >  4:307570/1‑137 (MQ=255)
                                                   tCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTATTAACCAACATAGGAGCAAGCCCCATGCCCCCCGTCAAATCCGCCATgcgc                                                                                >  2:213061/1‑137 (MQ=255)
                                                     aaCGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCtg                                                                              <  8:200647/137‑1 (MQ=255)
                                                       cGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCTTAACCAAATGTGGACCAAGCGACAAGCCCCCCGGCAATTCCGCAATTCGCtg‑tt                                                                           >  8:333587/1‑136 (MQ=255)
                                                       cGACAGCAAATACCGCCCTGCGCCACTGCTCACGGAAATGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCtg‑tg                                                                           <  4:51476/137‑1 (MQ=255)
                                                             cAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCTTGACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACt                                                                     >  6:163418/1‑137 (MQ=255)
                                                                     gCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGGTGTTGACCTACTGAGGAGCCACCCCCAAGCCCCCGGGGGATGCCGCCAAGCGCGG‑TGGCCACATTgtggag                                                             >  1:309617/1‑137 (MQ=255)
                                                                      cccTGCTCGAGTCCTCAAGTATTGGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGc                                                            <  5:251486/137‑1 (MQ=255)
                                                                        cTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCTACTGAGGCGCAAGCGCCCTGCCCCCCGGCAATGCCGCCATGCGCGG‑GGGCCACGTTGTGGAGCgc                                                          >  4:45852/1‑136 (MQ=255)
                                                                           cTCCAGTGCTCAAGAAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGt                                                       <  1:1240/137‑1 (MQ=255)
                                                                              cACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCTACTGAGGAGCAAGCGCCCTGCCCCCCGGCAATGCCGCAATGCGCTG‑TGGCAACATTGAGGAGCGACGT‑Gaa                                                   >  4:153482/1‑136 (MQ=255)
                                                                               aCTGCTCCAGGAAATGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GAcc                                                  <  8:261906/137‑1 (MQ=255)
                                                                                  ccTCAAGGAAATGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCggg                                               <  6:45414/136‑1 (MQ=255)
                                                                                   cTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTGTTAACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCCGGc                                              >  2:103397/1‑137 (MQ=255)
                                                                                   cTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCTTACACGACTGAGGAGCAAGCCCCAAGCCCCCCGTAAATGCCGCAAAGCGCGG‑GTCCAACATTGAGGAGCGACGT‑GACCgggg                                              >  7:95686/1‑136 (MQ=255)
                                                                                      aaGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATTCCGCAATGCGCGG‑TGCCAAATTTGAGGAGCGACGT‑GCCCGGGCCAt                                           >  8:117385/1‑137 (MQ=255)
                                                                                      aaGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTTTTCACCTAAAGAGGCGCAAGCGCCATGCCCCCCGGCAATTCCGCAATGCGGGG‑TGGCAACATTTGGGCGCGACGT‑GACCGGGCCaa                                           >  6:118166/1‑136 (MQ=255)
                                                                                      aaGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCTTGACCGACTGAGGAGGAAGCGCCCTGCCCCCCGTCAAAGCCGCAATGCGCTG‑TGGCAACTTTTAGGAGCGCCCT‑GACCGGGccca                                           >  8:34223/1‑135 (MQ=255)
                                                                                        ggAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCCCCATGCCCCCGGGCAATGCCGCAAAGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATgg                                         >  5:160784/1‑137 (MQ=255)
                                                                                        ggAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCCACAGAGGAGCAACCCCCAATCCCCCCGTGAAATCCCCAAATCGCTG‑TGCCCAATTTGAGGAGCGACGT‑GACCGGGCCAttg                                         >  4:33383/1‑135 (MQ=255)
                                                                                        ggAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGGTGTTCACCGACGGAGGCGCGACCCCCCAGCCCCCCGGCACTGCCGCCACTCGCGG‑GGGCACCGTTTAGGGGCGACGTGGCCCGGGCCagg                                          >  2:191150/1‑135 (MQ=255)
                                                                                        ggAAATGGTCGCGGCAGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTCACCAAATTAGGAGCAAGCCCCATGCCCCCCGGAAAATCCGCAAAGCGCAGTTCCCAACTTT‑ATGAGCGACGG‑GACCGGGGCATgg                                         >  8:357634/1‑137 (MQ=255)
                                                                                                tCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTTTTGACCGAATGAGGGGCAAGCGCCCTGCCCCCCGGGAATGGCCCAATGCGCGG‑GGGCAAAGTTGAGGAGCGACCT‑GGCCCGGCCATGGCAATGgtt                                 >  6:40418/1‑137 (MQ=255)
                                                                                                tCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGCCCCTCGGTGGGGGAAGCGCCCTGCCCCCCGGCCAAGCCGCAATGCGGGG‑TGGCCACATTGAGGGGCGGCCT‑GACCGGGCCATGGGAATGttt                                 >  2:257240/1‑137 (MQ=255)
                                                                                                tCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTCACCTACTGAGGAGCAAGCGCCAAGCCCCCGGGCAATGCCGCCATGCGCTG‑TGGCAAAGTTGAGGCGCGACGT‑GGCCGGGCCCTGGGATTGgtt                                 >  7:290164/1‑137 (MQ=255)
                                                                                                tCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGGTGACCTACTGAGGATCAAGCGCCAATACCCCCGTCCATGCCGCCCAGAGCTG‑TGGCAACTATTAGGAGCGACGT‑GGACGGGCCATGGCCtttttt                                 >  4:359625/1‑137 (MQ=255)
                                                                                                   cggccggcTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCGCCGACTGAGGAGCAACCGCCATGCCCCCCGGCAATGCCCCAATGCGCGG‑TGCCAACTTTGTGGAGCGCCGG‑TACCGGGCCCTGGGATTGTTTGcc                              >  8:129339/1‑137 (MQ=255)
                                                                                                     gccggcTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGa                            <  2:282119/137‑1 (MQ=255)
                                                                                                          gtggctggGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAgg                       <  7:129339/136‑1 (MQ=255)
                                                                                                          ctggctggGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAgg                       <  7:18306/137‑1 (MQ=255)
                                                                                                                ggggCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGATTGCCGAAAAGGGCTTTg                 <  7:54956/137‑1 (MQ=255)
                                                                                                                    cgcAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGCCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCa             <  1:197281/137‑1 (MQ=255)
                                                                                                                               tCGCGGGTTGTTGACCTACTGAGGGGCAAGCGCCAAGCCCCCCGTCAATTCCGCAATGCGCGG‑TGCCCACGTTGAGGAGCGCCGT‑GACCGGGCCATGGGATTGTTTGGCGAAAAGGGCTTTGGCCAGGTCAGCATgc  >  4:131381/1‑137 (MQ=255)
                                                                                                                                        |                                                                                                                                 
TGATCGGCCTGGACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGC  >  ACB143/4276893‑4277156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑