Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,277,0620GC35.3% 80.8 / 15.0 51P3P (CCG→CCC) fabR_1HTH‑type transcriptional repressor FabR
Reads supporting (aligned to +/- strand):  ref base G (11/22);  new base C (18/0);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 2.35e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCTGGCGGCCCATGTGGGGCTTACCGCCGGCT  >  ACB143/4276928‑4277192
                                                                                                                                      |                                                                                                                                    
cccTACCACCCAGGCTTCAACGACACCAAATCCCGCCCTGCGCCACTCCGCAAGGAAATGGCCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGt                                                                                                                                    <  8:140679/136‑1 (MQ=255)
   ttCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTTGTAACCGACTGGGGTGCAAGCGCCATTCCCCCCGGCaa                                                                                                                                 >  3:351754/1‑137 (MQ=255)
       aCGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCTTCTGGGGAGCAAGCGCCAATCCCCCCGTCAATGcc                                                                                                                             >  8:146666/1‑137 (MQ=255)
       aCGAAGGCTGCAACGACAGAAAATCCCGCCCTGCTCCACTGCTCAAGGAACTGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGcc                                                                                                                             <  3:130372/137‑1 (MQ=255)
        cgcaGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCGTAACCAACTGAGGCGCCAGACCCCTGCCCCCCGGCAATGccc                                                                                                                            >  5:332785/4‑136 (MQ=255)
            ggCTTCAACGACAGCAAATACCCCCCTGCTCCACTCCTCAAGCAACTGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAAt                                                                                                                        <  6:208590/137‑1 (MQ=255)
             gCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATg                                                                                                                       <  1:8887/137‑1 (MQ=255)
              cTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCGACTGAGGAGCAAGCGCCCAGCCCCCGGGCAAAGCCGCAATgc                                                                                                                      >  4:307570/1‑137 (MQ=255)
                tCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTATTAACCAACATAGGAGCAAGCCCCATGCCCCCCGTCAAATCCGCCATgcgc                                                                                                                    >  2:213061/1‑137 (MQ=255)
                  aaCGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCtg                                                                                                                  <  8:200647/137‑1 (MQ=255)
                    cGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCTTAACCAAATGTGGACCAAGCGACAAGCCCCCCGGCAATTCCGCAATTCGCtg‑tt                                                                                                               >  8:333587/1‑136 (MQ=255)
                    cGACAGCAAATACCGCCCTGCGCCACTGCTCACGGAAATGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCtg‑tg                                                                                                               <  4:51476/137‑1 (MQ=255)
                          cAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCTTGACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACt                                                                                                         >  6:163418/1‑137 (MQ=255)
                                  gCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGGTGTTGACCTACTGAGGAGCCACCCCCAAGCCCCCGGGGGATGCCGCCAAGCGCGG‑TGGCCACATTgtggag                                                                                                 >  1:309617/1‑137 (MQ=255)
                                   cccTGCTCGAGTCCTCAAGTATTGGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGc                                                                                                <  5:251486/137‑1 (MQ=255)
                                     cTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCTACTGAGGCGCAAGCGCCCTGCCCCCCGGCAATGCCGCCATGCGCGG‑GGGCCACGTTGTGGAGCgc                                                                                              >  4:45852/1‑136 (MQ=255)
                                        cTCCAGTGCTCAAGAAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGt                                                                                           <  1:1240/137‑1 (MQ=255)
                                           cACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCTACTGAGGAGCAAGCGCCCTGCCCCCCGGCAATGCCGCAATGCGCTG‑TGGCAACATTGAGGAGCGACGT‑Gaa                                                                                       >  4:153482/1‑136 (MQ=255)
                                            aCTGCTCCAGGAAATGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GAcc                                                                                      <  8:261906/137‑1 (MQ=255)
                                               ccTCAAGGAAATGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCggg                                                                                   <  6:45414/136‑1 (MQ=255)
                                                cTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTGTTAACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCCGGc                                                                                  >  2:103397/1‑137 (MQ=255)
                                                cTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCTTACACGACTGAGGAGCAAGCCCCAAGCCCCCCGTAAATGCCGCAAAGCGCGG‑GTCCAACATTGAGGAGCGACGT‑GACCgggg                                                                                  >  7:95686/1‑136 (MQ=255)
                                                   aaGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATTCCGCAATGCGCGG‑TGCCAAATTTGAGGAGCGACGT‑GCCCGGGCCAt                                                                               >  8:117385/1‑137 (MQ=255)
                                                   aaGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTTTTCACCTAAAGAGGCGCAAGCGCCATGCCCCCCGGCAATTCCGCAATGCGGGG‑TGGCAACATTTGGGCGCGACGT‑GACCGGGCCaa                                                                               >  6:118166/1‑136 (MQ=255)
                                                   aaGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCTTGACCGACTGAGGAGGAAGCGCCCTGCCCCCCGTCAAAGCCGCAATGCGCTG‑TGGCAACTTTTAGGAGCGCCCT‑GACCGGGccca                                                                               >  8:34223/1‑135 (MQ=255)
                                                     ggAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCCCCATGCCCCCGGGCAATGCCGCAAAGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATgg                                                                             >  5:160784/1‑137 (MQ=255)
                                                     ggAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCCACAGAGGAGCAACCCCCAATCCCCCCGTGAAATCCCCAAATCGCTG‑TGCCCAATTTGAGGAGCGACGT‑GACCGGGCCAttg                                                                             >  4:33383/1‑135 (MQ=255)
                                                     ggAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGGTGTTCACCGACGGAGGCGCGACCCCCCAGCCCCCCGGCACTGCCGCCACTCGCGG‑GGGCACCGTTTAGGGGCGACGTGGCCCGGGCCagg                                                                              >  2:191150/1‑135 (MQ=255)
                                                     ggAAATGGTCGCGGCAGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTCACCAAATTAGGAGCAAGCCCCATGCCCCCCGGAAAATCCGCAAAGCGCAGTTCCCAACTTT‑ATGAGCGACGG‑GACCGGGGCATgg                                                                             >  8:357634/1‑137 (MQ=255)
                                                             tCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTTTTGACCGAATGAGGGGCAAGCGCCCTGCCCCCCGGGAATGGCCCAATGCGCGG‑GGGCAAAGTTGAGGAGCGACCT‑GGCCCGGCCATGGCAATGgtt                                                                     >  6:40418/1‑137 (MQ=255)
                                                             tCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGCCCCTCGGTGGGGGAAGCGCCCTGCCCCCCGGCCAAGCCGCAATGCGGGG‑TGGCCACATTGAGGGGCGGCCT‑GACCGGGCCATGGGAATGttt                                                                     >  2:257240/1‑137 (MQ=255)
                                                             tCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTCACCTACTGAGGAGCAAGCGCCAAGCCCCCGGGCAATGCCGCCATGCGCTG‑TGGCAAAGTTGAGGCGCGACGT‑GGCCGGGCCCTGGGATTGgtt                                                                     >  7:290164/1‑137 (MQ=255)
                                                             tCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGGTGACCTACTGAGGATCAAGCGCCAATACCCCCGTCCATGCCGCCCAGAGCTG‑TGGCAACTATTAGGAGCGACGT‑GGACGGGCCATGGCCtttttt                                                                     >  4:359625/1‑137 (MQ=255)
                                                                cggccggcTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCGCCGACTGAGGAGCAACCGCCATGCCCCCCGGCAATGCCCCAATGCGCGG‑TGCCAACTTTGTGGAGCGCCGG‑TACCGGGCCCTGGGATTGTTTGcc                                                                  >  8:129339/1‑137 (MQ=255)
                                                                  gccggcTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGa                                                                <  2:282119/137‑1 (MQ=255)
                                                                       gtggctggGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAgg                                                           <  7:129339/136‑1 (MQ=255)
                                                                       ctggctggGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAgg                                                           <  7:18306/137‑1 (MQ=255)
                                                                             ggggCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGATTGCCGAAAAGGGCTTTg                                                     <  7:54956/137‑1 (MQ=255)
                                                                                 cgcAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGCCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCa                                                 <  1:197281/137‑1 (MQ=255)
                                                                                            tCGCGGGTTGTTGACCTACTGAGGGGCAAGCGCCAAGCCCCCCGTCAATTCCGCAATGCGCGG‑TGCCCACGTTGAGGAGCGCCGT‑GACCGGGCCATGGGATTGTTTGGCGAAAAGGGCTTTGGCCAGGTCAGCATgc                                      >  4:131381/1‑137 (MQ=255)
                                                                                                      ttcACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCTggc                            <  6:168205/137‑1 (MQ=255)
                                                                                                      ttcACCTACTGAGGAGCAAGCGCCAGGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGCCGG‑GACCGGGCCATGGCATTGTTTGCCGAAACGGGCTTTGGCCAGGTCATCATGCGCGAGCTggc                            <  2:221573/137‑1 (MQ=255)
                                                                                                          ccTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCTGGCGGcc                        <  3:153482/137‑1 (MQ=255)
                                                                                                               tGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCTGGCGGCCCAtgt                   >  1:62241/1‑137 (MQ=255)
                                                                                                                  ggagCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCTGGCGGCCCATGTggg                <  7:333587/137‑1 (MQ=255)
                                                                                                                   gagCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGa                                                                >  8:119731/1‑88 (MQ=255)
                                                                                                                   gagCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGa                                                                <  7:119731/88‑1 (MQ=255)
                                                                                                                      cAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCTGGCGGCCCATGTGGGGCtt            >  5:44932/1‑137 (MQ=255)
                                                                                                                           gcCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCTGGCGGCCCATGTGGGGCTTAccgc       >  1:289630/1‑137 (MQ=255)
                                                                                                                             cATGACCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCAGTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCTGGCGGCCCATGTGGTGCTTAccgccg     <  1:90285/137‑1 (MQ=255)
                                                                                                                                gCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCTGGCGGCCCATGTGGGGCTTACCGCCGGCt  <  3:362836/137‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
GCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTG‑TGCCAACTTTGAGGAGCGACGT‑GACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCATGCGCGAGCTGGCGGCCCATGTGGGGCTTACCGCCGGCT  >  ACB143/4276928‑4277192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑