Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,620,1460GC32.5% 37.2 / 12.6 42G269G (GGC→GGG) CJ019_05281hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (11/16);  new base C (1/12);  total (14/28)
Fisher's exact test for biased strand distribution p-value = 6.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTG‑CAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGT  >  ACB143/5620013‑5620270
                                                                                                                                      |                                                                                                                             
cgcCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCCGCTG‑CCGGCAAAAGTTGccc                                                                                                                            >  8:14755/1‑137 (MQ=255)
 gcCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTG‑CAGGCAAAAGTAGCCCa                                                                                                                           <  2:17422/137‑1 (MQ=255)
 gcCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCCGCCTGGGACGCCGCGGCCCGGCAAAA‑TAGcccc                                                                                                                           >  7:53165/1‑136 (MQ=255)
     tCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTG‑CAGGCAAAAGTAGCCCCGcgg                                                                                                                       >  2:119577/1‑135 (MQ=255)
      cgcgGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCAAGGCAGGCACCAG‑CAGGCCAAAGGAGCCCaccagt                                                                                                                      >  1:26649/1‑132 (MQ=255)
               ccGGTAACGGGAAGGGGTTTAAGTGGCGGAACACGCGGATATCGGCCTGGTGGGCTTCACGTGAGGCGGCCGGGTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTG‑CAGGCAAAAGTAGCCCAGC‑TGTATGCAAcgc                                                                                                            <  8:172275/137‑1 (MQ=255)
                     acgggaacgggTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCGGCATGGCAGGCAGCAG‑CAGGCAAAAGTAGCCCACC‑TGTATGCAACGCGACAcc                                                                                                      >  2:233204/1‑137 (MQ=255)
                         taacgggGTCAACTGGCGGAACACGCGTATAGCGGCCCGGTCGGCTCCACGTGAGGCGGCCGGCGGGTCGGGTGCGGCCGGCATGGCAGGCAGCTG‑CAGGCAAAAGACCCCCAGC‑TGTATCCAACGCGACTCCGGCa                                                                                                  <  6:5877/136‑1 (MQ=255)
                         caacgggTTGAACTGGCGCAACACGCGGATATCGGCCAGGTCGGCTTCCCGGGAGGCGGCCGGCTGGTCGGGGGCTGCCTGCATGGCAGGCAGGTG‑CAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCa                                                                                                  <  6:334105/136‑1 (MQ=255)
                                ttGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTTCCAGCCTGGCACGCAGCTG‑CAGGCAAAAGTATCCCAGC‑TGTATGCAACGCGACTCCGGCACCCCGGc                                                                                           >  2:97043/1‑137 (MQ=255)
                                   acggggCGCAACCCGCGGTTAGCGGCCTGGGGGGGTCCCCGTGCGGCGGCGGGGGGGGGGGGGGCGGCCTGCAGGGCTGGCAGCTG‑CAGGCAAAAGACCCCCCGC‑TGTATCCACCGCGACTCCGGCACGCCGGCCCa                                                                                        <  2:165665/133‑1 (MQ=255)
                                    aCTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCAGCATGGCAGGCAGCTG‑CAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTACGGCACGCCCGCCCaa                                                                                       >  6:89421/1‑137 (MQ=255)
                                           gAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCGGCATGGCAGGCACCTG‑CCGGCAAAAGTACCCCATC‑TGTAAGCCACGCGACTCCCGCCCGCCGGGCCAACGCCGCa                                                                                >  8:97006/1‑137 (MQ=255)
                                                cgcgGATATCGGCCAGGTCGGCTTCACGTGTGGCGGCGGGCGGGGCGGGTGCTGCCTGCATGGCAGGAAGCTG‑CAGGCAAAATTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCacgacg                                                                           <  8:276633/137‑1 (MQ=255)
                                                   gtatagCGCCCAGGGCGGCTTCCCGGGCGGCGGCCGGCTGGTCGGGTGCGGCCTGCATGGCAGGCAGCGG‑ACGGCAAAATTCCCCCCGC‑GGAATCCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATg                                                                        <  4:251141/135‑1 (MQ=255)
                                                                  cGGCTTCACGTGAGGAGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTG‑CACGCAAAAGTAGCCCCGA‑TGGATGCAACGAGAATCCGGAAAGCCGGCCCAACGCGGCACCAACATGCCGTCCCACGCAAGc                                                         >  8:71704/1‑137 (MQ=255)
                                                                   ggCTTCAGGTCCGGCGGCCGGCTGGGGGGGTGCTGCCTGCAGGCCAGCCAGCGG‑CAGCCAAAAGTCCCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCa                                                        <  8:53165/137‑1 (MQ=255)
                                                                   ggCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTG‑CAGGCAAAAGTAGCCCCGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCa                                                        <  2:259339/137‑1 (MQ=255)
                                                                                  ggccggcTGGTCGGGTGCTGCCTGCATGGCACGCAGCAG‑CAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCCGCACGCCGGCCCAACGCGGCCCGACGATGCTGTCCCACGCACGCAGGCGCCCCATAAGgc                                         >  6:180059/1‑137 (MQ=255)
                                                                                  ggccggcTGGGCGGGTGCGGCCTGCATGGCAGGCAGCTG‑CAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGgc                                         <  6:128353/137‑1 (MQ=255)
                                                                                  gcccggcGGGGCGGGGGCGGCCTGCCTGGCAGGCAGCGG‑CAGCCAAAAGTAGCCCCGC‑TGTATCAAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGgc                                         <  2:354609/135‑1 (MQ=255)
                                                                                      ggcTGGTCGGGTGCTGCCTGGATGGCAGGCAGCTG‑CAGGCAAAAGTAGCCCCGC‑TGTATGCAACGCGCCGCCGGCACGCCGGCCCCACGCGGCCCGCCGCTGGTGTCCCAGGCCCGCCGGCGCCCCGTCCGgcgcgc                                     >  2:53451/1‑137 (MQ=255)
                                                                                      ggcTGGTCGGGTGCTGCCGGCATGGGAGGCAGCGG‑CAGGGAAAAGTATCCCCCCGTGT‑TGCAACCCGCCACCCGCCCGCCCGCCCCACCCGGCCCCACGATGCTGTGCCCGGCCCCCCGGCGCCCCATCCGgcgcgc                                     >  2:284875/1‑137 (MQ=255)
                                                                                        gggggcGGGGGCGGCCGGCAGGCCCGGCAGCTG‑CCGCCAAAAGCCCCCCCGC‑TGTAGCCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcgg                                   <  1:119577/132‑1 (MQ=255)
                                                                                             ggggTGCTGCCTGCATGGAAGGCAGCTG‑CAGGAAAAAGTAGCCCACC‑TGTATGAACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcctcg                              <  8:50568/136‑1 (MQ=255)
                                                                                             ggggTGCGGCCGGCATGCCTGGCAGCTC‑CAGCCAAAAGTCCCCCCGC‑TGTAGCCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcctcg                              <  1:310375/136‑1 (MQ=255)
                                                                                                  gcggcctgcAGGCCAGCCCGGTG‑CAGCCAAAAGACCCCCCGC‑TTTAGCCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCtt                         <  3:20152/134‑1 (MQ=255)
                                                                                                   ctgcctgcTTGGCAGGCTGCTG‑CAGCCAAAAGTACCCCCCC‑TGTAGCCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCttt                        <  2:26649/137‑1 (MQ=255)
                                                                                                        ggcAGGGCAGCAAGCTG‑CCGGAAAAAGTCGCCCCGT‑TGTATGCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAg                   <  4:3818/136‑1 (MQ=255)
                                                                                                         gcATGGCAGGCAGCGG‑CAGGCAAAAGTAGCCCAGC‑TGTATTCAAACCGCCTACCGCCCCCCGGCCCAACCCCGCACCCCCAATCTGTACCCGGCCCGCACGCCCCCCATAAGGCGCGCGGCCACCGCCATTTGCAgg                  >  2:223138/1‑137 (MQ=255)
                                                                                                          cagggcaggcaGCGG‑CAGCCAAAAGTACCCCAGC‑TGTATCCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGc                 <  3:115629/134‑1 (MQ=255)
                                                                                                          aaTGGCAGGCAGCGG‑CAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGc                 <  8:57646/136‑1 (MQ=255)
                                                                                                             ggcaggcaGCTG‑CAGGAAAAAGTACCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCa              <  5:77842/137‑1 (MQ=255)
                                                                                                              gcaggcagCGG‑CAGGCAAAAGAAGCCCAGC‑TGTATGCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCAt             <  7:46999/137‑1 (MQ=255)
                                                                                                               caggctgCTG‑CCGGCAAACGTAGCCCAGC‑GGTTTGCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATc            <  1:227700/137‑1 (MQ=255)
                                                                                                                  gcagCGG‑CAGCAAAATGTCCCCCCGC‑TTTATCAACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATctgc         <  3:321381/137‑1 (MQ=255)
                                                                                                                     gCTG‑CAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTgg      >  7:118922/1‑137 (MQ=255)
                                                                                                                      gctgacggCAAATGTAGCCGAGC‑GGAAGCCAACCCGACGCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTgg      <  6:345872/131‑1 (MQ=255)
                                                                                                                          cagtaaaaaGTACCCCACC‑TGAATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGt  <  7:71709/132‑1 (MQ=255)
                                                                                                                          cAGGCAAACGTAGCCCAGC‑TGTATGCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGt  <  7:71704/137‑1 (MQ=255)
                                                                                                                          cAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGt  <  5:180059/137‑1 (MQ=255)
                                                                                                                          cAGGCAAAAGTAGCCCAGC‑TGTATCCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGt  <  5:383171/137‑1 (MQ=255)
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CGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTG‑CAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGT  >  ACB143/5620013‑5620270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑