Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,693,1750AC44.4% 8.0 / 12.4 47S236R (AGC→CGC) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (8/17);  new base C (3/17);  total (12/35)
Fisher's exact test for biased strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAG‑ATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCG  >  ACB143/5693041‑5693309
                                                                                                                                       |                                                                                                                                      
tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGGGGGCGTTAGCCAC‑AACACCacc                                                                                                                                      >  1:335224/1‑135 (MQ=255)
tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAG‑ATCACCAgc                                                                                                                                      >  6:186052/1‑137 (MQ=255)
tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGGGCCCTTCCGCCCG‑AAAACCacc                                                                                                                                      >  6:201480/1‑135 (MQ=255)
     cACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCCG‑ATCACCAGCGcccc                                                                                                                                 >  8:25345/1‑136 (MQ=255)
                cATGGCCAGCTGCCGCGGCCTGCCGGGCATCCCGGCGCGCGCCGAAACCGTGGCCCGGGCGCGCAACCTGCTGCGGGTGGAACAGAGCGGCGGGCGGGCGCACTTCAGCCAG‑ACCCCCCGCGCCCGGGGCGCGCGGc                                                                                                                      <  8:31906/137‑1 (MQ=255)
                 aTGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTGCAGCCAG‑ATCACCAGCGCCCGTGGCGCGCGGCt                                                                                                                     <  5:227731/137‑1 (MQ=255)
                 aTGGCCAGCTGCCTCGGCGGGCCGGGCATCCCGGAGAGCGCCGAAACCGGGGCCCTGGCGCGCAACCTGCGGCTGGGGCAACAGCGCGGCGGGCGGGCGCACTGCACCCAG‑AACACCCGCGCCCGGGGCGCGCGGCt                                                                                                                     <  6:207728/137‑1 (MQ=255)
                     ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGGGGCCCGGGCGCGCAACCTGCGGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAG‑ATCACCAGCGCCCGTGGGGCGCGGCTGATc                                                                                                                 <  6:88027/137‑1 (MQ=255)
                          ttCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAG‑ATCACCCGCGCCCGTGGCGCGCGGCTGCTCgcccc                                                                                                            >  7:184486/1‑136 (MQ=255)
                           gccTCGGCGGGCCGGTCATCCAGGAGAGCGCCGAAACGGTGGCCCTGGCGGGAACCCGGCTGCTGGGGTAACCTCGCGGGGGGGGGGCGCACTTCAGCCAG‑ATCACCCGCGCCCGTGTCGCGCGGCTGATCgcccag                                                                                                           <  2:180416/136‑1 (MQ=255)
                           cccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAG‑ATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccag                                                                                                           <  6:291977/136‑1 (MQ=255)
                            ccTCGGCCTGCCGGGCATCCCGGCGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAT‑ATCACCCGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                          <  7:36545/137‑1 (MQ=255)
                            ccTCGGCCTGCCGGGCACCCCGGAGAGCGCCGAAACCGGGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAG‑ATCACCCGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                          <  7:30863/137‑1 (MQ=255)
                             cTCGGCCTGCCGGGCTTCCCGGAGAGCGCCGAACCCGGGGCCCTGGCGCGCAACCTGCTGCTGGTGCACCAGCGCGGCGGGCGTGCGCACTTCAGCCAG‑ATCACCAGCGCCCGGGGCGCGCGGCTGATCgcccaggc                                                                                                         <  3:194443/137‑1 (MQ=255)
                                 gCCTGCCGGGCAGCCCGGCTAGCGCCGAAACCGTGGCCCGGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGGGTGCGTGCGCACTTCAGCCAG‑ATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccaggcccag                                                                                                     <  2:324847/137‑1 (MQ=255)
                                  ccTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAG‑ATCACCAGCGCCCGTGGCGCGCGGCTGATCGcccaggcccagg                                                                                                    <  5:186052/137‑1 (MQ=255)
                                      ccGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGCGCCCTTCAGCCCG‑ATCACCACCCCCCGTGGCGCGCGGCTGATCGCCccggccccggccgt                                                                                                >  3:144993/1‑135 (MQ=255)
                                      ccGGGCATCCCGGAGAGCGACGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGCTGGAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGCACCACC‑CCCCCCCAGGCGCCCGCCTGAACGCCCAGGCCCCCGCCtt                                                                                                >  2:265355/1‑137 (MQ=255)
                                         ggCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAG‑CTCACCAGCGCCCCTGGCGCGCGGCTGATCGCCCCCGCCCCGGCCGTggg                                                                                             >  8:41126/1‑137 (MQ=255)
                                           cATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCGGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAG‑ATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGcc                                                                                           <  7:88624/137‑1 (MQ=255)
                                                     ggcgcGGAAACCGGGGCCCTGGCGGGCAACGTGGTGCGGGTGCAACAGAGCGGGGTGCGTGCGCAGTTCACCCAG‑ATCACCGGCGCCGGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                 <  4:28502/136‑1 (MQ=255)
                                                     cgcgcCGAACCCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGTAACAGCGCGGGGTGCGGGCGCACTTCACCCAG‑ACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                 <  2:335224/136‑1 (MQ=255)
                                                     cgcgcCGAAAGCGGGGCCCTGGCGCGCAACCTGCTGCGGGGGCAACAGAGCGGCGTGCGGGCCCACTACACCCAC‑ATCCCCCGCGCCCGTGGCGCGCGGCAGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                 <  7:211716/136‑1 (MQ=255)
                                                     agCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAG‑ATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                 <  1:37870/137‑1 (MQ=255)
                                                        gcCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGTGGCCCGTCAGCCAC‑ATAACCCCCGCCCCGGGCGCGCGGCTGATCGCCCCGGCCCAGGCCTTGGGCCTGCCGGTTAccgc                                                                              >  5:345085/1‑137 (MQ=255)
                                                          cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAG‑ATAACCAGCGCCCCTGGCGCGCGGCTGATCGCCCACGCCCCGGCCGTGGGCCTGCCGGTAAccgccc                                                                            >  8:22286/1‑136 (MQ=255)
                                                          cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCTTCAGCCAG‑CTCACCCGCGCCCCGGGCGCGCGGCTGATCGCCCCGGCCCAGGCCTTGGGCGTGCCGGTAAcagccg                                                                            >  4:91367/1‑137 (MQ=255)
                                                             aaCCGTGGCCCGGGCGCGCAACCTGCTGCGGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAG‑ATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATg                                                                         <  8:122440/137‑1 (MQ=255)
                                                               ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGTGTGCGCACTTCAGCCAG‑ATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  1:47988/137‑1 (MQ=255)
                                                               ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAG‑ATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  2:7674/137‑1 (MQ=255)
                                                               ccGTGGCCCTGGCGCGCAACCGGCTGCTGGTGGACCAGAGCGGCGGGCGGGCGCACTTCAGCCAG‑ATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  8:44738/137‑1 (MQ=255)
                                                               ccGGGGCCCTGGCGCGCAACGTGCTGCGTGTGGAACCGAGCGGGGGGGGTGGGCAGTTCCGCCAG‑ATCACGAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  2:359916/137‑1 (MQ=255)
                                                                    gCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAG‑AGCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt                                                                  <  6:269843/137‑1 (MQ=255)
                                                                        tGGCGCGCAACCTGCTGCTGGTGTAACAGAGCGGCGTGCGTGCGAACTTCAGCAAG‑ATCACCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTAc                                                              <  7:25345/137‑1 (MQ=255)
                                                                                           gggggAACCGCGCGGCGTGCGTGCGCACTTCCGCCAG‑ATCCCCACCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACg                                           <  4:252026/134‑1 (MQ=255)
                                                                                                   tgagCGGCTGGCTTGCGCACTTCACCCAC‑ATCCCCCGGGCCGGTGCCGCGCTGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg                                   <  3:177355/136‑1 (MQ=255)
                                                                                                     cgcggcgGGCGTGCGCACTTCAGCCCG‑AGCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                                 <  7:124423/136‑1 (MQ=255)
                                                                                                      ggggcgTGCGTGCGCACTGCCCCCAC‑AGCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                                <  7:22234/135‑1 (MQ=255)
                                                                                                      gcggcgTGCGTGCGCACTTCAGCCAG‑ATAACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCACGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTTATACTCACCGACGACTCGCTgcgc                                >  6:158573/1‑137 (MQ=255)
                                                                                                      gcggcgGGCGGGCCCACTTCACCCCC‑AGCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                                <  7:27661/137‑1 (MQ=255)
                                                                                                          ggtgcgtgcGCACTTCAGCCAG‑ATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                            <  8:206272/136‑1 (MQ=255)
                                                                                                          ggggcgtgcgCACTTCAGCCAG‑AACACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                            <  1:207632/134‑1 (MQ=255)
                                                                                                          cgggcgtgcgCACTGCAGCCAG‑AGCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                            <  7:61133/134‑1 (MQ=255)
                                                                                                            ggctggcgaACTGCAGCCAC‑CCCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc                          <  4:329570/132‑1 (MQ=255)
                                                                                                               gggcgcACTTCCCCCAT‑AACACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                       <  1:175099/135‑1 (MQ=255)
                                                                                                                       ttCAGCCAG‑ATCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTa               <  2:220841/137‑1 (MQ=255)
                                                                                                                       ttCAGCCAG‑ATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTa               <  6:167838/137‑1 (MQ=255)
                                                                                                                            ccAG‑ATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTCCCACg          >  4:71290/1‑137 (MQ=255)
                                                                                                                                     ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgccg  >  8:108119/1‑137 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAG‑ATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCG  >  ACB143/5693041‑5693309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑