Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,386,519	G→A	intergenic (‑528/+1123)	CJ019_05070 ← / ← nptII	hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,386,519	0	G	A	100.0%	69.9 / NA	22	intergenic (‑528/+1123)	CJ019_05070/nptII	hypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/9);  total (13/9)

CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑‑GGA‑TC‑‑AT‑‑C‑CGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCT  >  ACB143/5386410‑5386607                                                                                                                           |                                                                                         gcctttttATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGcc                                                                   <  5:75039/134‑1 (MQ=255)    tttttATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGccccc                                                                <  2:21790/137‑1 (MQ=255)              aaaCAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCAc                                                      <  2:8392/136‑1 (MQ=255)                            cGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt                                        >  4:361102/1‑137 (MQ=255)                             gAATACTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTGGGCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCtttt                                       <  7:245488/137‑1 (MQ=255)                                  ttttCATTTAACACCCTAATATCACATCTCGCCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGT‑‑‑GAACGGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                                  <  1:170691/136‑1 (MQ=255)                                       aTTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCt                             <  1:325080/137‑1 (MQ=255)                                        tttAATACCGTAATATGACATCTCGGGAGGCTTGTCGACGACGGCGGTCTCAGTGGTGT‑‑‑CA‑‑GGT‑AC‑‑ATACC‑CCGACGCTAGCAAAGCATGAGAAAGCCACCGGAATATCTCGTTCCGGGGGCTTTTTTATTGCGCTg                            <  8:4602/137‑1 (MQ=255)                                                   aaTATGACATCTCGGCCGGCTTTGCGACGACGGGGGTCTCAGTGGGGTA‑‑CT‑‑GTACAA‑‑AC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg                 <  5:360596/137‑1 (MQ=255)                                                    atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAggg                >  8:161605/1‑137 (MQ=255)                                                     tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc               >  4:296994/1‑137 (MQ=255)                                                     tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc               >  6:388511/1‑137 (MQ=255)                                                      atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCg              >  5:155029/1‑137 (MQ=255)                                                             ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTTCGATGCGGGTGCCAGGGCGTGCCCtt       >  6:156043/1‑137 (MQ=255)                                                             ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt       >  8:189773/1‑137 (MQ=255)                                                             ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGAACACCTTCCGGGGGCGTTTTTATTTGGCAGCGGGTGCCAGGGCGGGCCCtt       >  1:194952/1‑137 (MQ=255)                                                             ctcGGCCGGCTTGTCGACGACGGCGGTCTAAGT‑‑GGTGTACG‑‑GTA‑CA‑‑AA‑‑CACCTACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt       >  6:374407/1‑137 (MQ=255)                                                             ctcGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt       <  5:355881/137‑1 (MQ=255)                                                                ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCCCCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg    >  5:148875/1‑137 (MQ=255)                                                                ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg    >  1:305794/1‑137 (MQ=255)                                                                ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg    >  1:117363/1‑137 (MQ=255)                                                                  ccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCAGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCt  >  1:243328/1‑137 (MQ=255)                                                                                                                           |                                                                                         CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑‑GGA‑TC‑‑AT‑‑C‑CGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCT  >  ACB143/5386410‑5386607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑