Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	179,484	T→C	C316R (TGC→CGC)	CJ019_00182 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	179,484	0	T	C	100.0%	226.9 / NA	63	C316R (TGC→CGC)	CJ019_00182	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/45);  total (18/45)

CTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCTGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGT  >  ACB143/179352‑179614                                                                                                                                     |                                                                                                                                   ctgcGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAg                                                                                                                                >  5:195695/1‑137 (MQ=255)   gcGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGACAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGcc                                                                                                                              >  2:97236/1‑137 (MQ=255)       gTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                          <  5:297150/137‑1 (MQ=255)       gTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                          <  4:306027/137‑1 (MQ=255)         tCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcggcg                                                                                                                        >  4:356792/1‑137 (MQ=255)         tCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcggcg                                                                                                                        >  3:76366/1‑137 (MQ=255)                ctggctggCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                 <  1:178660/137‑1 (MQ=255)                ctggctggCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                 <  5:115877/137‑1 (MQ=255)                   gctggcCTGAACGCCCGCCTTGGCGGCCAGCGTCCCGACGAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACGAACGACCTCATTTCACGCAGCCGCGGCGGCGAGTTTgg                                                                                                              >  1:311809/1‑136 (MQ=255)                     tggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAg                                                                                                            >  1:134588/1‑137 (MQ=255)                      ggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCCGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAgg                                                                                                           >  3:331329/1‑136 (MQ=255)                         cTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGTTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACAAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCt                                                                                                        <  3:21704/137‑1 (MQ=255)                          tGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTg                                                                                                       >  6:234649/1‑137 (MQ=255)                           taaCGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTTCTTGTGCCAGCTACCCGGAAACCAACGAGCTCATTTCCCGGAGCCGCGGCGGCGAGTTTGCAGTGCTgg                                                                                                      <  4:320086/136‑1 (MQ=255)                           gAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTgg                                                                                                      <  1:198413/137‑1 (MQ=255)                               gcccgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCAcc                                                                                                  >  3:10953/1‑137 (MQ=255)                                 cctccTTGGCGGCCATCGTAACGAAGAGTGTGTGCAAGCTGTAGTGGAACAGCTGCGTAGTACTTGTGCCAGCTAACATGCCACCAACGAACTCATTTCCCGCAGCCTCGGCTGCGAGTTTGCAGTGCTGGCACAgg                                                                                                <  8:141659/134‑1 (MQ=255)                                 ccgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  6:255366/137‑1 (MQ=255)                                 ccgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  6:386480/137‑1 (MQ=255)                                 ccgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  7:147529/137‑1 (MQ=255)                                 ccgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  8:118914/137‑1 (MQ=255)                                 ccgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  3:312924/137‑1 (MQ=255)                                 ccgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  8:122372/137‑1 (MQ=255)                                 ccgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  5:2716/137‑1 (MQ=255)                                  cgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                               <  8:192264/137‑1 (MQ=255)                                    ccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCa                                                                                             >  4:12953/1‑137 (MQ=255)                                             ccAGCGTACCGACCAGTTGTTTCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    <  2:294706/137‑1 (MQ=255)                                             ccAGCGTACCGACCAGTTGTTTCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    <  1:97236/137‑1 (MQ=255)                                             ccAGCGTACCGACCAGTTGTTGCAAGCTGTAGGTGAACAGCTGCTTCGTACTTGTGACAGCTACCCGGAAAACAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    <  4:191730/137‑1 (MQ=255)                                             ccAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    <  1:267981/137‑1 (MQ=255)                                              cAGCGTACCGACCATTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATga                                                                                   <  8:151032/137‑1 (MQ=255)                                              cAGAGTAGCGACCAGTTGTTGAAATCGGTAGGTGAACAGCTTCGTATGACTTGTGCAAGCTACCCGGAAACCCACGACCTCTTTTCCCGCAGCCGCGGCTGCGAGTTTGCAGTGCTGGCACCGGGCATGTTGCATga                                                                                   <  3:259187/137‑1 (MQ=255)                                                 cGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATga                                                                                   <  4:60885/134‑1 (MQ=255)                                                 cGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATga                                                                                   >  3:60885/1‑134 (MQ=255)                                                  gTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATgaggag                                                                               >  6:322261/1‑137 (MQ=255)                                                   tACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGaggagg                                                                              <  5:203473/137‑1 (MQ=255)                                                    aCCGAGCAGTTGTAGCATGCGGTAGGTGTACAGCTGCGGCGTACTTGTGCCAGCTACACGGACACCAACGACCTCATTTCCCGCAGCCGCGGCGGGGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGc                                                                             <  6:78368/137‑1 (MQ=255)                                                     ccGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCa                                                                            <  1:280666/137‑1 (MQ=255)                                                     ccGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCa                                                                            <  5:234649/137‑1 (MQ=255)                                                        aCCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTa                                                                         <  4:76366/137‑1 (MQ=255)                                                             ttggtgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCt                                                                    <  5:99731/137‑1 (MQ=255)                                                               gttgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  7:326907/137‑1 (MQ=255)                                                               gttgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  8:125521/137‑1 (MQ=255)                                                               gttgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  6:147232/137‑1 (MQ=255)                                                                   cAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGt                                                                                                           >  1:212295/1‑92 (MQ=255)                                                                   cAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGt                                                                                                           <  2:212295/92‑1 (MQ=255)                                                                              tGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAgg                                                   <  4:103167/137‑1 (MQ=255)                                                                               gAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGc                                                  <  4:10953/137‑1 (MQ=255)                                                                                     cTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGtt                                            <  3:321450/137‑1 (MQ=255)                                                                                       gCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGc                                          >  1:361575/1‑137 (MQ=255)                                                                                         gtcgtACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCag                                        <  3:12953/137‑1 (MQ=255)                                                                                             tACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGcc                                    >  4:361069/1‑137 (MQ=255)                                                                                             tACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGcc                                    >  6:351273/1‑137 (MQ=255)                                                                                              aCTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCt                                   <  4:113678/137‑1 (MQ=255)                                                                                              aCTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCt                                   <  5:48764/137‑1 (MQ=255)                                                                                                ttGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                               <  6:261522/75‑1 (MQ=255)                                                                                                ttGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                               >  5:261522/1‑75 (MQ=255)                                                                                                        gCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACg                         <  3:148450/137‑1 (MQ=255)                                                                                                          tACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                               >  4:238492/1‑65 (MQ=255)                                                                                                          tACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                               <  3:238492/65‑1 (MQ=255)                                                                                                                          aCCTGATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGATGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGAc       <  6:195695/137‑1 (MQ=255)                                                                                                                          aCCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGAc       <  6:167522/137‑1 (MQ=255)                                                                                                                               aTTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCAAGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  5:66671/137‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   CTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCTGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGT  >  ACB143/179352‑179614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑