Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 4,759,509 G→A intergenic (+124/‑16) fpvA_3 → / → ligM_KToptimized Ferripyoverdine receptor/sequence generated by OPTIMIZER for KT2440

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,759,5090GA100.0% 120.1 / NA 35intergenic (+124/‑16)fpvA_3/ligM_KToptimizedFerripyoverdine receptor/sequence generated by OPTIMIZER for KT2440
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/20);  total (15/20)

ACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTC  >  ACB143/4759375‑4759640
                                                                                                                                      |                                                                                                                                   
aCAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGt                                                                                                                                   <  5:325644/137‑1 (MQ=255)
  aGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                                 <  1:235479/137‑1 (MQ=255)
  aGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                                 <  6:325572/137‑1 (MQ=255)
  aGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                                 <  5:163946/137‑1 (MQ=255)
  aGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                                 <  3:66661/137‑1 (MQ=255)
    cTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag                                                                                                                               <  3:133233/137‑1 (MQ=255)
            cGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                                 >  8:48005/1‑127 (MQ=255)
            cGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                                 <  7:48005/127‑1 (MQ=255)
            cGCCTGACGTTGAACGCAAAAAACCCCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTAGTATAATGTGTGGAATTTTGAGAGGATAAAAATTTCACAAAAAAACATAGAGTTTAGGATGtata                                                                                                                       >  3:176096/1‑137 (MQ=25)
                 gACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGa                                                                                                                  >  2:47199/1‑137 (MQ=255)
                  aCGTTGAACGAAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAg                                                                                                                 <  2:13545/137‑1 (MQ=255)
                             aaaaaaCCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTa                                                                                                      <  1:245494/137‑1 (MQ=255)
                                     gCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAg                                                                                              >  8:371406/1‑137 (MQ=255)
                                            ggTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGc                                                                                       <  7:371406/137‑1 (MQ=255)
                                                         taGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACAAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCcggcgg                                                                          <  1:47199/136‑1 (MQ=255)
                                                                           catcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGtt                                                        <  4:69869/137‑1 (MQ=255)
                                                                            atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTg                                                       >  1:316266/1‑137 (MQ=255)
                                                                                  ccGTATAAGGTGTGGAATTGTCAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGATGTATACATGAGCGCCCGGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAAc                                                 <  1:103194/136‑1 (MQ=39)
                                                                                             gtgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCACCACCGTTGAAATGTTGCGTAACTCCCAGATTgg                                      >  4:308269/1‑137 (MQ=255)
                                                                                               ggAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                                 >  2:306010/1‑44 (MQ=255)
                                                                                               ggAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                                 <  1:306010/44‑1 (MQ=255)
                                                                                               ggAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcg                                    <  5:384413/137‑1 (MQ=255)
                                                                                                 aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGcc                                  >  1:1675/1‑137 (MQ=255)
                                                                                                        aGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCt                           >  1:3842/1‑137 (MQ=255)
                                                                                                             aTAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCg                      >  1:278583/1‑137 (MQ=255)
                                                                                                             aTAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCG‑CCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCgg                     <  5:150651/137‑1 (MQ=255)
                                                                                                               aaCAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgt                    <  5:99816/137‑1 (MQ=255)
                                                                                                                aCAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgtc                   >  6:218998/1‑137 (MQ=255)
                                                                                                                aCAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgt                    >  7:29065/1‑136 (MQ=255)
                                                                                                                aCAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgt                    <  8:29065/136‑1 (MQ=255)
                                                                                                                   aTTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTg                                                                                                 >  2:90096/1‑56 (MQ=255)
                                                                                                                   aTTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTg                                                                                                 <  1:90096/56‑1 (MQ=255)
                                                                                                                     ttCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTcgc              >  4:285675/1‑137 (MQ=255)
                                                                                                                              acacATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGtt     >  5:189044/1‑137 (MQ=255)
                                                                                                                                 caTAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTtctc  <  2:231687/137‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
ACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTC  >  ACB143/4759375‑4759640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑