Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	4,713,494	Δ2 bp	100%	coding (1668‑1669/1905 nt)	htpG ←	Chaperone protein HtpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	4,713,494	0	T	.	100.0%	225.8 / NA	47	coding (1669/1905 nt)	htpG	Chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (20/27);  total (20/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*	ACB143	4,713,495	0	C	.	100.0%	225.8 / NA	47	coding (1668/1905 nt)	htpG	Chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (20/27);  total (20/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGTGCGACAGCTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCATCTGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAGTCGCTCGACCAAA  >  ACB143/4713359‑4713612                                                                                                                                        ||                                                                                                                      tgtgCTACAGCTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTTGACTCCGGCACTTTCTGCCCGCTGGATTCCAGGATCTGGCGCAt                                                                                                                        <  2:242874/136‑1 (MQ=255) tgtgCGACCGCTCGTCGAAACGGGCTGCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCAt                                                                                                                        <  4:55261/136‑1 (MQ=255) tgtgCGACAGCTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCAt                                                                                                                        <  7:219167/136‑1 (MQ=255)  gtgCGACAGCTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCAt                                                                                                                        <  8:136733/135‑1 (MQ=255)      gACAGCTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCAtgcagg                                                                                                                   <  8:279334/136‑6 (MQ=255)         aGCTCGGCGAAACGGTCGTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGccc                                                                                                              <  3:245143/136‑1 (MQ=255)            tCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAgg                                                                                                           >  6:149451/1‑136 (MQ=255)            tCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAgg                                                                                                           >  6:344561/1‑136 (MQ=255)              ggCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTc                                                                                                         <  1:291390/136‑1 (MQ=255)              ggCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCCGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCAGGCGCA‑‑TGCAGGCCCAGGTc                                                                                                         >  2:313305/1‑136 (MQ=255)                cGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCGCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCt                                                                                                       <  2:366424/136‑1 (MQ=255)                                    tCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATc                                                                                   >  8:348173/1‑136 (MQ=255)                                     cGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCg                                                                                  >  2:133258/1‑136 (MQ=255)                                     cGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCg                                                                                  >  6:314787/1‑136 (MQ=255)                                     cGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCg                                                                                  >  1:333397/1‑136 (MQ=255)                                     cGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCg                                                                                  >  8:88651/1‑136 (MQ=255)                                      gggATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTGGAACTCGACGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGc                                                                                 <  4:254479/134‑1 (MQ=255)                                      gTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCTCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGc                                                                                 >  7:114549/1‑136 (MQ=255)                                         aTCCAGCTGCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCgg                                                                              <  7:67041/136‑1 (MQ=255)                                           ccAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGc                                                                             >  5:274527/1‑135 (MQ=255)                                           ccAGCTTCTCGATCAGCGGGTGGCTCGGGGTGAACTCGAAGATCGGCTTGGACTCCGGCACTTGCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCg                                                                            <  2:188614/136‑1 (MQ=255)                                            cAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCg                                                                            <  6:105533/135‑1 (MQ=255)                                            cAGCTTCTCGATCAGAGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCg                                                                            <  8:371265/135‑1 (MQ=255)                                               cTTCTCGATCAGCGGGTGGCTCGGGGTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTc                                                                        <  7:124661/136‑1 (MQ=255)                                                     gATCCGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTc                                                                    <  6:285306/134‑1 (MQ=255)                                                     gATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGt                                                                     <  7:72000/133‑1 (MQ=255)                                                       tCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGc                                                                >  4:222537/1‑136 (MQ=255)                                                         aGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCgg                                                              <  5:170189/136‑1 (MQ=255)                                                               tGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCgag                                                        <  3:355591/136‑1 (MQ=255)                                                               tGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCgag                                                        <  7:88651/136‑1 (MQ=255)                                                                 gCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGAc                                                      <  8:50707/136‑1 (MQ=255)                                                                           aaCTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTc                                             <  8:60675/135‑1 (MQ=255)                                                                            aCTCGAAGTGCGGCCTGGACTCCGGCACTTTCTGCCCGCGGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCCGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCgg                                           <  8:135404/136‑1 (MQ=255)                                                                            aCTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCgg                                           <  4:189952/136‑1 (MQ=255)                                                                            aCTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCgg                                           <  8:132313/136‑1 (MQ=255)                                                                            aCTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGAGCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCgg                                           <  8:175296/136‑1 (MQ=255)                                                                            aCTCGAAGATCGGCTTGGACTCCGGCACTTGCTGCCCGCTGGCTTCCAGGAGCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCgg                                           <  8:315231/136‑1 (MQ=255)                                                                               cGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCa                                        >  8:353718/1‑136 (MQ=255)                                                                                   gATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCt                                    >  1:332183/1‑136 (MQ=255)                                                                                    aTCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTg                                   >  5:121022/1‑136 (MQ=255)                                                                                       ggCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTc                                 <  5:188742/135‑1 (MQ=255)                                                                                        gCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCAc                               <  1:341733/136‑1 (MQ=255)                                                                                           tGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCAcc                              >  1:49323/1‑134 (MQ=255)                                                                                                     cACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCtt                   >  7:335404/1‑135 (MQ=255)                                                                                                      aCTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCttt                  >  6:352622/1‑135 (MQ=255)                                                                                                          tCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAg               <  3:333598/134‑1 (MQ=255)                                                                                                           cTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAGTCg            >  2:44966/1‑136 (MQ=255)                                                                                                                cGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAGTCGCTCGa       <  3:222537/136‑1 (MQ=255)                                                                                                                     gCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAGTCGCTCGACCaaa  >  4:109806/1‑136 (MQ=255)                                                                                                                     gCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAGTCGCTCGACCa    <  4:241185/134‑1 (MQ=255)                                                                                                                     gCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAGTCGCTCGACCa    <  5:371993/134‑1 (MQ=255)                                                                                                                     gCTTCCAGGATCTGGCGCA‑‑TGCAGGCACAGGTCCTGCTCGCCAATGGCCAGGATCGCAGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAGTCGCTCGACaaaa  >  6:118715/1‑136 (MQ=255)                                                                                                                                        ||                                                                                                                      TGTGCGACAGCTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCATCTGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAGTCGCTCGACCAAA  >  ACB143/4713359‑4713612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑