Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,020,866:1 +C 100% coding (599/1005 nt) tagO → putative undecaprenyl‑phosphateN‑acetylglucosaminyl 1‑phosphate transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,020,8661.C92.5% 167.3 / 7.6 53coding (599/1005 nt)tagOputative undecaprenyl‑phosphateN‑acetylglucosaminyl 1‑phosphate transferase
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base C (21/27);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

GCATCGCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATACTTT  >  ACB143/2020734‑2020988
                                                                                                                                     |                                                                                                                            
gCATCGCCAGTATCGAGGCCATATGCGTGTGCATGGGCGGTAGCCTGCTCTACTGGGTGACTGGGCATGCCTAGGGAGCGTGGCTGCCGATATTACTGGCGGGCACTGTTGCGTGCGTCCTTTGCTGGAAtttgc                                                                                                                             <  6:131804/135‑3 (MQ=255)
    cGCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTccccccc                                                                                                                      >  3:22172/1‑136 (MQ=255)
     gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCATCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGTGCTTCCTGTGCTGGAATTTccccccc                                                                                                                      <  5:336435/135‑1 (MQ=255)
     gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTcccccccc                                                                                                                     >  1:166767/1‑135 (MQ=255)
     gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTcccccccc                                                                                                                     <  1:126568/136‑2 (MQ=255)
     gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTcccccccc                                                                                                                     >  5:317972/1‑135 (MQ=255)
     gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTcccacccc                                                                                                                     >  5:315123/1‑135 (MQ=255)
     gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTccaccccc                                                                                                                     >  2:52309/1‑135 (MQ=255)
       cAGTATCGAGGCCATCTGAGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTccccccccgc                                                                                                                   >  6:119068/1‑133 (MQ=255)
                  ccATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGCGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTa                                                                                                        >  8:236231/1‑136 (MQ=255)
                  ccATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTa                                                                                                        >  1:295635/1‑136 (MQ=255)
                    aTCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATg                                                                                                      <  8:261640/136‑1 (MQ=255)
                    aTCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATg                                                                                                      >  2:387945/1‑136 (MQ=255)
                    aTCTGCGTGTGCATGGGCGGAAACCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATg                                                                                                      <  4:128319/136‑1 (MQ=255)
                       tGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGc                                                                                                   >  6:295572/1‑136 (MQ=255)
                                  gggCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGAt                                                                                                <  6:69955/128‑1 (MQ=255)
                                  gggCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGAt                                                                                                >  5:69955/1‑128 (MQ=255)
                                   ggCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGCTATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGGTGGAATTTCCCCCCCCGCACGTATTTTTAGTGGCGATGCCGGGAgg                                                                                       >  6:383283/1‑135 (MQ=255)
                                     cGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTgg                                                                                     <  1:387945/136‑1 (MQ=255)
                                               ctctACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTgg                                                                             <  6:66326/134‑1 (MQ=255)
                                               ctctACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTgg                                                                             <  5:371764/134‑1 (MQ=255)
                                               ctctACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTgg                                                                             <  6:207973/134‑1 (MQ=255)
                                               ctctACTGGCTCACTGGTAATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCttg                                                                             <  4:169350/134‑3 (MQ=255)
                                               cgctACTGGCTCCCTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTTGCGGCCACTGTGGCGGTCGTCCGGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTgg                                                                             <  8:191386/132‑1 (MQ=255)
                                                 ctACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTa                                                                          >  5:274716/1‑135 (MQ=255)
                                                 ctACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTAt                                                                         >  2:23216/1‑136 (MQ=255)
                                                 ctACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTAt                                                                         >  7:305856/1‑136 (MQ=255)
                                                    cTGGCTCACTGGTCATGACGAGGCAGCGGGGCGGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGt                                                                      <  4:71928/136‑1 (MQ=255)
                                                       gCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGc                                                                    >  1:274907/1‑135 (MQ=255)
                                                       gCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGc                                                                    >  7:35744/1‑135 (MQ=255)
                                                       gCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGGATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGc                                                                    >  7:294036/1‑135 (MQ=255)
                                                          cACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGGATTTTTATGGGCGATGCCGGCAGGGGATTTCTGGGTATCGTGGTAgg                                                                >  5:227195/1‑136 (MQ=255)
                                                          cACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTACCCCCCCGCACGGATTTTTATGGGCGATTCCCGCAGTGGCTTTATTGGTTTAGTGcaatg                                                                >  6:236662/1‑132 (MQ=255)
                                                            cTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCTCTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTTGGTATCGTGCTAGGgc                                                              <  6:246304/136‑1 (MQ=255)
                                                            cTGGGCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGgc                                                              <  1:349223/136‑1 (MQ=255)
                                                                  aTGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGTTgg                                                        <  2:168158/136‑1 (MQ=255)
                                                                  aTGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTgg                                                        <  5:67240/136‑1 (MQ=255)
                                                                     ccGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCACCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTTCTAGGGCTGCTGGCAt                                                     >  7:77090/1‑135 (MQ=255)
                                                                            aGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGc                                              <  6:292211/136‑1 (MQ=255)
                                                                            aGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGc                                              <  3:380151/136‑1 (MQ=255)
                                                                             gcttGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCGGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCa                                             >  6:164772/4‑136 (MQ=255)
                                                                                 ggCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCt                                         >  1:368480/1‑136 (MQ=255)
                                                                                      ccGATATTTCTTGCGGGCTCTGTGGCGGGCTTACTGTGCTGGAATTTCCCCCCCGGACGTTTTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGGATTAGTGGCAGGCTGGGTaa                                   <  5:324254/136‑1 (MQ=255)
                                                                                      ccGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGt                                     >  4:134316/1‑135 (MQ=255)
                                                                                       cGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTaa                                   <  1:306090/136‑1 (MQ=255)
                                                                                       cGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTaa                                   <  1:72596/136‑1 (MQ=255)
                                                                                         ttatTACTGGCGGCATCGGGGGCGGGGTTCCTGTGTTGTTATTTCCGGCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGTGTAAAc                                 <  8:383251/135‑1 (MQ=255)
                                                                                         atatTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAAc                                 <  4:389296/136‑1 (MQ=255)
                                                                                              aCTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGc                             <  2:274907/135‑1 (MQ=255)
                                                                                              aCTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGc                             <  7:299257/135‑1 (MQ=255)
                                                                                              aCTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGc                             <  3:134316/135‑1 (MQ=255)
                                                                                              aCTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGc                             <  3:381228/135‑1 (MQ=255)
                                                                                                     gcCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTc                     >  7:155976/1‑136 (MQ=255)
                                                                                                       cACTGTGGCGTGCTTCCTGTGCTGGAATTTCCCCCCCAGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTATGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGTGTAAACCCTCTATTATTCtt                   <  6:280433/136‑2 (MQ=255)
                                                                                                       cACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTg                   <  2:244088/136‑1 (MQ=255)
                                                                                                       cACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTg                   <  5:236662/136‑1 (MQ=255)
                                                                                                       cACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCGGGGTATCGTGCTTGGGCTGCGGGCATTAGTGGCAGGCTGGGTTAACCCGCTATTATTCTg                   >  2:104876/1‑136 (MQ=255)
                                                                                                            tgGCGGGCTGCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGTCAGGCTGGGTAAACCCGCTATTATTCTGGGggt              <  2:107902/136‑1 (MQ=255)
                                                                                                                    ttCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAAt       >  1:207735/1‑135 (MQ=255)
                                                                                                                    ttCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATa      >  3:259294/1‑136 (MQ=255)
                                                                                                                       cTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCAGTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATACtt   >  7:359688/1‑136 (MQ=255)
                                                                                                                          tgCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATACttt  <  5:258315/134‑1 (MQ=255)
                                                                                                                                     |                                                                                                                            
GCATCGCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATACTTT  >  ACB143/2020734‑2020988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: