| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 570,448 | A→C | 34.6% | D599A (GAC→GCC) | fdoG_2 → | Formate dehydrogenase‑O major subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 570,448 | 0 | A | C | 34.6% | 22.0 / 5.5 | 39 | D599A (GAC→GCC) | fdoG_2 | Formate dehydrogenase‑O major subunit |
| Reads supporting (aligned to +/- strand): ref base A (14/11); new base C (11/3); total (25/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTGGGCCTGGGCCTGGCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTCCCCGAGCACTACGA > ACB143/570316‑570577 | aCTGGGCCTGGGCCTGGCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCCCGACGt > 7:158062/1‑136 (MQ=255) ctgggcctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGCCCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCGCCGCCGGGCCGGAc > 3:388297/1‑136 (MQ=255) ctgggcctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAACGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCCCCGTGCCGGAc > 7:126195/1‑136 (MQ=255) ctgggcctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCCCGCCGTGCCggtc > 2:147893/1‑134 (MQ=255) ctgggcctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCCCCGACGTGCCGGAc > 7:92971/1‑136 (MQ=255) ctgggactggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGa > 2:79356/1‑135 (MQ=255) cctggCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTc > 2:55008/1‑134 (MQ=255) gccAACCCCCGCATCCTATACACCCGTCCCCCCACCGACCCCCAAGGCACGCCGGGGCACCCGCAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACt < 7:311923/136‑1 (MQ=255) aaCCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGCCGGGCCGGACTTGAAGGTCGACTCGCCGCCCGAGGCGGGGATg > 7:348812/1‑135 (MQ=255) gCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGCCGTGCCGGACTTCAAGGTCGACTCGCCCCCCGAGGCGGGGATGAAcccgt > 7:223072/1‑134 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGCCGTGCCGGCCTTCAAGGTCGACGCGCCGCCCCAGGCGGGGCTGAACCCatt > 1:379534/1‑135 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGCCGTGCCGGACTTCAAGGTCGACTCGCCGCCCGCGGCGGGGATGAACCCttt > 8:51314/1‑135 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGCCGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGCACCCttt > 2:119178/1‑135 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGGCGTCCAGGTCGACTCGCCGCCCGAGGCGGGGCTGAACCCttt > 8:51319/1‑135 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGTTGCACCCttt > 3:267746/1‑135 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGCACCCgtt > 8:15280/1‑135 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCttt > 3:125612/1‑135 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCCGCCCCGACGTGCCGGACTTCAACGTCGACTCCCCGCCCGAGGCGGGGATGAACCCgtt > 6:122493/1‑135 (MQ=255) cTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCCGCCCCGACGGGCCGGACGTCAAGGTCGACTCGCCCCCCGAGGCGGGGATGCACCCttt > 5:334403/1‑135 (MQ=255) ccAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTtca > 6:396649/1‑135 (MQ=255) cAGCGACCCGCAAGGAACGCCGTGGGACCCGGATAAAACGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTtcatc < 5:204020/136‑1 (MQ=255) gCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGCCGGGGCGGCCAGCAAGGGCGCCACGCCGCCCCCCGCGGGGCGGAACCCGGTcatcat > 8:137722/1‑136 (MQ=255) gCCGTGGCACCCGTAGAAAACGCGCCTGGTATGGGGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTg < 3:326807/136‑1 (MQ=255) cccGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCCGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCCCCCGAGGCGGGGGTGCACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGGttc > 2:2215/1‑136 (MQ=255) agaAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTATTCGc > 5:280737/1‑136 (MQ=255) cgcCGGGAATGGTGGAACGGCAAGCCCGGGACCGGCACCGACGGGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACa < 4:261932/136‑1 (MQ=255) gcCTGGTATGGTGGAACGGCAAGGCCTGGACCCGCACCGCCGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCATTCATCATGAACCCCGAAGGCGTGGCGCGTTTGTTCGCCCTCGACa > 6:398757/1‑135 (MQ=255) ggAACGCCAAGGCCTGCCGCGCCACCCCCGAGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAgg < 3:346278/136‑1 (MQ=255) caCGGCAAGGCCTGGACGGGCACCGACGTGCCGGACTTCAAGGGCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGcc < 2:70088/135‑1 (MQ=255) aaCGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGcc < 1:276395/136‑1 (MQ=255) ggCAAGGCCTGGACCGGCCCCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGGTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGt < 5:322702/136‑1 (MQ=255) ggCAAGGCCGGGACCGGCACCGACGTGCGGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGt < 5:95506/136‑1 (MQ=255) cAAGGCCTGGACCGGCACCGCCGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATTGCCGCAGGCCCGtta > 3:221408/1‑135 (MQ=255) acggCCGGGCCCGCCCCCGCCGGGCCGCACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTcc < 1:400315/134‑1 (MQ=255) ggCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTcccc < 7:24648/136‑1 (MQ=255) gCCTGGACCGGCACCGCCGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTCCCCg < 3:219719/136‑1 (MQ=255) ccTGGACCGGCCCCGACGTGGCGGCCGTCAAGGTCGACTCGCCGCCCCCGGCGGGGCTGTGCCCGGTCATCATGTCCCCCGCCGGCGTGGCGGGGTTCTTAGGCAACGCCCAGAGGGCCGCACGCCCGGGCCCCgc > 6:55120/1‑135 (MQ=255) aCCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTCCCCGAGCACt < 5:390627/136‑1 (MQ=255) gCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTCCCCGAGCACTACGa < 4:399460/136‑1 (MQ=255) | ACTGGGCCTGGGCCTGGCCGGCCAACCGCCGCATCCTTTACAACCGTGCCTCCAGCGACCCGCAAGGCAAGCCGTGGGACCCGGAGAAAAAGCGCCTGGTATGGTGGAACGGCAAGGCCTGGACCGGCACCGACGTGCCGGACTTCAAGGTCGACTCGCCGCCCGAGGCGGGGATGAACCCTTTCATCATGAACCCCGAAGGCGTGGCGCGTTTCTTCGCCATCGACAAGATGGCCGAAGGCCCGTTCCCCGAGCACTACGA > ACB143/570316‑570577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |