Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 605,516 C→T 8.6% A572A (GCG→GCA dxs ← 1‑deoxy‑D‑xylulose‑5‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143605,5160CT 8.6% 253.1 / 11.9 81A572A (GCG→GCAdxs1‑deoxy‑D‑xylulose‑5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (37/37);  new base T (3/4);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

CCGGCCGCATCCAGCCCGCACTCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGC  >  ACB143/605382‑605647
                                                                                                                                      |                                                                                                                                   
ccGGCCGCATCCAGCCCGCACTCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGccc                                                                                                                                     <  3:280668/135‑1 (MQ=255)
ccGGCCGCATCCAGCCCGCACTCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGccc                                                                                                                                     <  1:291093/135‑1 (MQ=255)
ccGGCCGCATCCAGCCCGCACTCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGccc                                                                                                                                     <  3:298866/135‑1 (MQ=255)
ccGGCCGCATCCAGCCCGCACTCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGccc                                                                                                                                     <  4:189561/135‑1 (MQ=255)
ccGGCCGCATCCAGCCCGCACTCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCcg                                                                                                                                    <  7:344829/136‑1 (MQ=255)
  ggCCGCATCCAGCCCGCACTCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGccc                                                                                                                                     <  1:57837/133‑1 (MQ=255)
   gCCGCATCCAGCCCGCACTCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCTgcgc                                                                                                                                 <  7:14048/136‑1 (MQ=255)
          ccAGCCCGCACTCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCat                                                                                                                          >  1:320179/1‑136 (MQ=255)
              cgcGCACTCAGCCAGCATTTGCTCAGTCTTGTCTTGCTCTACTTAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCTCTGCCTGGCTGGCAAGGAACTCAGCCACCGCAGCGCCCGCGCCACCCattttg                                                                                                                      <  6:188846/134‑5 (MQ=255)
              cccGCACTCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGTTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCatgatg                                                                                                                      <  5:122210/136‑1 (MQ=255)
                cGCACTCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGc                                                                                                                    <  1:319663/136‑1 (MQ=255)
                     tCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCcgc                                                                                                                                   >  5:338428/1‑116 (MQ=255)
                     tCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCcgc                                                                                                                                   <  6:338428/116‑1 (MQ=255)
                     tCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTtct                                                                                                               >  3:188669/1‑136 (MQ=255)
                     tCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTtct                                                                                                               >  3:117588/1‑136 (MQ=255)
                       agccagcATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTtct                                                                                                               <  1:317052/134‑1 (MQ=255)
                        gccagcATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCCTGATGGCGTTCTCtt                                                                                                            <  6:387271/136‑1 (MQ=255)
                        gccagcATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCtt                                                                                                            <  5:285300/136‑1 (MQ=255)
                         ccagcATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCCCCCACCGCAGCGCCCCCGCCACCCATGATGGCGTtcacatc                                                                                                           >  5:241425/1‑131 (MQ=255)
                          cagcATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTc                                                                                                           >  1:108291/1‑135 (MQ=255)
                                   cgcAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTAc                                                                                                  <  1:322320/135‑1 (MQ=255)
                                      aGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACcagc                                                                                              <  8:301978/136‑1 (MQ=255)
                                      aGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACcag                                                                                               >  2:326729/1‑135 (MQ=255)
                                          ttGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCTGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCt                                                                                          >  3:98610/1‑136 (MQ=255)
                                             gCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCAt                                                                                       <  6:324093/136‑1 (MQ=255)
                                                tGCTCGACATAGATATCCGGCAGCCCCAGGTGCGGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGATCTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGc                                                                                    >  5:174353/1‑136 (MQ=255)
                                                tGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTCCCAGCAGCTCATGGc                                                                                    >  1:85132/1‑136 (MQ=255)
                                                     gACATAGATCTCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACAATCAGCTCATGGctgcct                                                                               <  1:248836/136‑1 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTTACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  3:309000/1‑136 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCTGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               <  7:210313/136‑1 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCTGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  8:369826/1‑136 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCTGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcc                                                                                <  8:60915/135‑1 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               <  1:15349/136‑1 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  8:67527/1‑136 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  5:62798/1‑136 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  6:107950/1‑136 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  8:43911/1‑136 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  6:291164/1‑136 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               <  6:3062/136‑1 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  8:209377/1‑136 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               <  8:100415/136‑1 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  7:100415/1‑136 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  3:154622/1‑136 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  4:102581/1‑136 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               <  4:76417/136‑1 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               <  1:292741/136‑1 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcc                                                                                <  2:399794/135‑1 (MQ=255)
                                                     gACATAGATATCCGGCAGCCCCAGGTGCACAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  6:10027/1‑136 (MQ=255)
                                                      aCATAGATATCCGGCAGCCCCATGTGCAGAAGCGGCGTGAGCACTGCCTGGCTTGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcctg                                                                              <  2:292596/136‑1 (MQ=255)
                                                           gATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGc                                                                         <  6:209692/136‑1 (MQ=255)
                                                                  ggCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCa                                                                  <  8:11306/136‑1 (MQ=255)
                                                                    cAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCAc                                                                 >  3:89335/1‑135 (MQ=255)
                                                                    cAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCAc                                                                 >  2:345197/1‑135 (MQ=255)
                                                                       ccccAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAgc                                                             <  6:294981/136‑1 (MQ=255)
                                                                          cAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGcc                                                          >  4:199075/1‑136 (MQ=255)
                                                                              tGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGt                                                      <  4:170817/136‑1 (MQ=255)
                                                                                    aGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCg                                                >  2:233345/1‑136 (MQ=255)
                                                                                    aGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCAGCCAGCTCTAGCACCAGCGCCTCGTCCAGCg                                                <  4:238865/136‑1 (MQ=255)
                                                                                     gCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCTGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCgg                                               >  2:293903/1‑136 (MQ=255)
                                                                                           tGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               <  8:104159/98‑1 (MQ=255)
                                                                                           tGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGctgcct                                                                               >  7:104159/1‑98 (MQ=255)
                                                                                           tGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGAc                                         >  5:160198/1‑136 (MQ=255)
                                                                                             aGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACg                                        <  3:238339/135‑1 (MQ=255)
                                                                                             aGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACg                                        <  7:124595/135‑1 (MQ=255)
                                                                                             aGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGa                                       <  1:318392/136‑1 (MQ=255)
                                                                                                aCTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGctc                                                                       >  7:131758/1‑101 (MQ=255)
                                                                                                aCTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGctc                                                                       <  8:131758/101‑1 (MQ=255)
                                                                                                     ctggctggCAAGGAACTCACCCACCGCAGCGCCCGCGcc                                                                                                                                >  1:79944/1‑39 (MQ=255)
                                                                                                     ctggctggCAAGGAACTCACCCACCGCAGCGCCCGCGcc                                                                                                                                <  2:79944/39‑1 (MQ=255)
                                                                                                     ctggctggCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATg                               >  5:32106/1‑136 (MQ=255)
                                                                                                     ctggctggCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATg                               >  7:3102/1‑136 (MQ=255)
                                                                                                          tggcAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGac                          >  4:364334/1‑136 (MQ=255)
                                                                                                            gcAAGGAACTCACCCACCGCAGCGCCTGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGacca                        <  5:216635/136‑1 (MQ=255)
                                                                                                            gcAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGCAGAGCATGTCGacca                        >  4:296067/1‑136 (MQ=255)
                                                                                                            gcAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGacca                        <  2:66124/136‑1 (MQ=255)
                                                                                                            gcAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGacca                        <  1:233345/136‑1 (MQ=255)
                                                                                                            gcAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCGCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGacca                        >  3:328693/1‑136 (MQ=255)
                                                                                                                 gAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTg                   >  5:15621/1‑136 (MQ=255)
                                                                                                                 gAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTg                   >  5:273501/1‑136 (MQ=255)
                                                                                                                 gAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTg                   <  5:267112/136‑1 (MQ=255)
                                                                                                                      cacccaccGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGtt              <  7:336689/136‑1 (MQ=255)
                                                                                                                      cacccaccGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGtt              <  5:147975/136‑1 (MQ=255)
                                                                                                                       acccaccGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGtt              <  7:261781/135‑1 (MQ=255)
                                                                                                                             cGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGt       <  8:365847/136‑1 (MQ=255)
                                                                                                                               cAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGACGCGCATGTCTCCCACCGGGGCGTTGATCTGTTc     >  4:896/1‑136 (MQ=255)
                                                                                                                               cAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTc     <  7:262101/136‑1 (MQ=255)
                                                                                                                               cAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTc     >  6:63593/1‑136 (MQ=255)
                                                                                                                                  cgcCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGa                                          <  7:69525/96‑1 (MQ=255)
                                                                                                                                  cgcCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGa                                          >  8:69525/1‑96 (MQ=255)
                                                                                                                                  ccgccGCGCCAACCATGTAGGGGTTACGCTCGAATGTTTACAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc  <  8:361171/133‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
CCGGCCGCATCCAGCCCGCACTCAGCCAGCATTTGCGCAGGCTTGGCATGCTCGACATAGATATCCGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGC  >  ACB143/605382‑605647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: