Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	616,680	G→T	14.3%	T17T (ACC→ACA)	CJ019_00584 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	616,680	0	G	T	14.3%	198.4 / 5.0	69	T17T (ACC→ACA)	CJ019_00584	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/38);  new base T (9/2);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 6.01e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAATCTCCAAGGATGGCCGCACGTG  >  ACB143/616550‑616808                                                                                                                                   |                                                                                                                                 cATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGt                                                                                                                                <  3:208583/133‑1 (MQ=255) cATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGACGTGTAGTATCCTCTCCAGTAATTATTTTATCTGAGTAAATTTTGTAgg                                                                                                                             >  1:87564/1‑135 (MQ=255) cATTTTCATGGTGGTGCAGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGt                                                                                                                                <  2:78430/133‑1 (MQ=255)     ttCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCAc                                                                                                                          <  5:129394/135‑1 (MQ=255)            tggtgCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATGCTCTCCAGTAATTCTTTGATCTGAGTAATTTTTGTTGGCACCGCatgat                                                                                                                  >  8:254547/1‑136 (MQ=255)              gtgCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCAtgatga                                                                                                                <  1:79109/136‑1 (MQ=255)              gtgCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCAtgatga                                                                                                                <  3:71213/136‑1 (MQ=255)                       tGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCCTCGGCACCCGCAGAGCCGTGGCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGt                                                                                                         <  7:171842/134‑1 (MQ=255)                       tGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTGTTAACCTCTCCAGTAATTGTTTGATAGTAGGAATTTTGGTTGGCCCCGCTAGATGATATGAGTGc                                                                                                       >  6:70927/1‑136 (MQ=255)                         cATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAg                                                                                                     <  1:217714/136‑1 (MQ=255)                            cACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGGGGTATACTCTCCAGTTATTCTTTGATATGAGTTATTTTTGTTGCCACCTCATGATGATAGGCGTGGAGctc                                                                                                  >  3:86910/1‑136 (MQ=255)                                aTGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTc                                                                                              <  6:366496/136‑1 (MQ=255)                                    aaGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTATTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAAt                                                                                           >  8:266312/1‑135 (MQ=255)                                    aaGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATACTCTCCAGTAATTCTTTGATCAGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCGCTTTCAAt                                                                                           >  8:236523/1‑135 (MQ=255)                                    aaGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGGGTTATGCTCTGCAATAATTATTTTATATGAGTTATTTTGGTAGCCACCACAAGATGATAGGCGTGCAGCGCTTTCAAt                                                                                           >  1:303712/1‑135 (MQ=255)                                    aaGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGGGTTATACTCTACAGTTATTATTTTATATGATTAATTTTTGTAGCACCCACATGATTTTAGGGGTGCAGCTCGTTCaaa                                                                                           >  6:262061/1‑134 (MQ=255)                                      ggCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAg                                                                                        <  7:311442/136‑1 (MQ=255)                                      ggCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGGGTTATCCACTCCAGTAATTCTTTGATCTGTGTAATTTTTGTAGCCACCTCATGATGATAGGCGTGGCGCTCTTTGTATAAg                                                                                        >  7:378309/1‑136 (MQ=255)                                         ttCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTGTTTAACGCGGCACTTATTATTTTGTTTGTGTAATTTTTTTTTACACCTCAATATGTTAGGCGTGCATCTGTTTAAATaaatcg                                                                                     >  6:182836/1‑132 (MQ=255)                                           ccAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCg                                                                                     <  7:178453/134‑1 (MQ=255)                                            cAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTATTTGATCTGAGTAATTTTTGTAGCCACCACATGATGAATGGGGGGCAGCTCTTTAAATAAGGCGtt                                                                                   >  2:265671/1‑135 (MQ=255)                                              gTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCAt                                                                                <  4:214147/136‑1 (MQ=255)                                              gTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCAt                                                                                <  3:374987/136‑1 (MQ=255)                                              gTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCAt                                                                                <  4:122058/136‑1 (MQ=255)                                              gTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGGCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGGTCAt                                                                                >  8:234449/1‑136 (MQ=255)                                                 gCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGc                                                                              <  4:316181/135‑1 (MQ=255)                                                 gCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGc                                                                              <  3:51822/135‑1 (MQ=255)                                                    tAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTATTTTATCTGAGTAATTTTTGTATCCACCTCATGATGATAGGCGTGGAGAGCTTTCAATAATGCGTTGATGCTAAg                                                                          >  2:105589/1‑136 (MQ=255)                                                     aaaCAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTTATCTGAGTAAATTTTGTAGACACCTCATGATGATAGGCGTGCAGCTCTTTCAAAAAGGCGTTCATTCTAAg                                                                          >  3:103470/1‑135 (MQ=255)                                                          acaGCTTTCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCtca                                                                    <  6:154175/136‑1 (MQ=255)                                                          acaGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCtca                                                                    <  4:405932/136‑1 (MQ=255)                                                                        tCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAAc                                                      <  2:248954/136‑1 (MQ=255)                                                                        tCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAAc                                                      <  2:101818/136‑1 (MQ=255)                                                                          ggCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCg                                                    <  3:394331/136‑1 (MQ=255)                                                                          ggCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCg                                                    <  5:291344/136‑1 (MQ=255)                                                                           gCACCCTCAGAGCCGTGTCTTATCCTCTCCAGTAAATCTTTGATATGAGTAATTTTTGTAGCTACCTCATTATTATAGTCTTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGt                                                   <  8:223780/136‑1 (MQ=255)                                                                                      gCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTaaa                                        <  7:254547/136‑1 (MQ=255)                                                                                      gCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTaaa                                        <  2:293559/136‑1 (MQ=255)                                                                                                 tCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGtaat                              <  4:86910/135‑1 (MQ=255)                                                                                                 tCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGtaat                              <  7:183646/135‑1 (MQ=255)                                                                                                   ctctCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGtaataa                            <  5:122743/135‑1 (MQ=255)                                                                                                   ctctCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGtaataa                            <  7:196114/135‑1 (MQ=255)                                                                                                   ctctCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGtaataa                            <  1:216962/135‑1 (MQ=255)                                                                                                   ctctCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGtaataa                            <  1:345200/135‑1 (MQ=255)                                                                                                      tcCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCaa                        >  2:190282/1‑136 (MQ=255)                                                                                                       ccAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGtaataa                            >  4:248123/1‑131 (MQ=255)                                                                                                       ccAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGtaataa                            <  3:248123/131‑1 (MQ=255)                                                                                                         aGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTAc                                                <  2:366457/109‑1 (MQ=255)                                                                                                         aGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTAc                                                >  1:366457/1‑109 (MQ=255)                                                                                                         aGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAAtct                     >  4:359335/1‑136 (MQ=255)                                                                                                         aGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAAtct                     >  1:111546/1‑136 (MQ=255)                                                                                                         aGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAAtct                     >  6:193612/1‑136 (MQ=255)                                                                                                         aGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAAtct                     >  7:370542/1‑136 (MQ=255)                                                                                                         aGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAAtc                      >  7:365219/1‑135 (MQ=255)                                                                                                         aGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATAAAAGTAATAAGCAAtct                     >  3:272939/1‑136 (MQ=255)                                                                                                         aGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTAATTATGATATGAGTGCAGCTCTTTCAAAAAGTCGTTAATGCTAAGACATCATCACTACATCAAACATTTACAGATAAAATATAAGTAATAAGAAAtc                      >  1:212611/1‑135 (MQ=255)                                                                                                              ttCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGt                                                   <  4:160904/101‑1 (MQ=255)                                                                                                              ttCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGt                                                   >  3:160904/1‑101 (MQ=255)                                                                                                                   tGATCTGAGTAATTTTGGTAGAAACATAATTATGATAGGCGTGCAGCTCTTTCAATATTGCTTTCATGCTAAGTCCTAATCACTACATCAAACAGTTACAGATTAAATATAAGTAATAAGAAAGCTCCAAGGATgg           >  6:360101/1‑136 (MQ=255)                                                                                                                    gATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAATCTCCAAGGATgg           <  1:265671/135‑1 (MQ=255)                                                                                                                     aTCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAATCTCCAAGGATGGcc         >  7:201302/1‑136 (MQ=255)                                                                                                                          aGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAATCTCCAAGGATGGcc         <  6:263318/131‑1 (MQ=255)                                                                                                                          aGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAATCTCCAAGGATGGcc         <  4:172807/131‑1 (MQ=255)                                                                                                                          aGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAATCTCCAAGGATGGCCGCACg    >  7:28827/1‑136 (MQ=255)                                                                                                                          aGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAATCTCCAAGGATGGCCGCACg    <  5:314989/136‑1 (MQ=255)                                                                                                                          aGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAATCTCCAAGGATGGCCGCACg    <  5:114446/136‑1 (MQ=255)                                                                                                                          aGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAATCTCCAAGGATGGCCGCACg    <  7:60554/136‑1 (MQ=255)                                                                                                                           gTAATTTTTGTATCCACCTCATGATGATATGCGTGCAGCTCTTTCAATAAGTCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAATCTCCAAGGATGGCCGCACg    <  2:212611/135‑1 (MQ=255)                                                                                                                            tAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTTCAGATTAAATATAAGTAATAAGCAATCTCCAAGGATGGCCGCACgtg  >  8:294259/1‑136 (MQ=255)                                                                                                                                   |                                                                                                                                 CATTTTCATGGTGGTGCCGCTCTGCATCACCATGCAAGGCTTCCAGTTGCTTAAACAACAGCTTGCAGTCATCGGCACCCGCAGAGCCGTGTCTTATCCTCTCCAGTAATTCTTTGATCTGAGTAATTTTGGTAGCCACCTCATGATGATAGGCGTGCAGCTCTTTCAATAAGGCGTTCATGCTAAGTCCTCATCACTACATCAAACCGTTACAGATTAAATATAAGTAATAAGCAATCTCCAAGGATGGCCGCACGTG  >  ACB143/616550‑616808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑