Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	1,227,173	A→G	67.7%	V389A (GTC→GCC)	glpK ←	Glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	1,227,173	0	A	G	67.7%	89.3 / 56.7	65	V389A (GTC→GCC)	glpK	Glycerol kinase
Reads supporting (aligned to +/- strand):  ref base A (12/9);  new base G (19/25);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01

CGCATCTGCGGCCGCTCCACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCGGTAAAGG  >  ACB143/1227038‑1227305                                                                                                                                        |                                                                                                                                     cgcATCTGCGGCCGCTCCACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGa                                                                                                                                      >  4:350121/1‑136 (MQ=255) cgcATCTGCGGCCGCTCCACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGa                                                                                                                                      >  6:198332/1‑136 (MQ=255)                 ccACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTgg                                                                                                                        >  2:339575/1‑134 (MQ=255)                  cACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAg                                                                                                                     <  1:147086/136‑1 (MQ=255)                  cACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAg                                                                                                                     <  5:99052/136‑1 (MQ=255)                  cACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAg                                                                                                                     <  1:93860/136‑1 (MQ=255)                     gCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCg                                                                                                                  <  6:334141/136‑1 (MQ=255)                       aGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGAt                                                                                                                <  5:380955/136‑1 (MQ=255)                       aGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGAt                                                                                                                >  6:22564/1‑136 (MQ=255)                          tGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGa                                                                                                             <  1:9585/136‑1 (MQ=255)                            ccGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACt                                                                                                           >  5:386120/1‑136 (MQ=255)                             cGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCTTCGCGGGTCTGGTAGGCGATGGACTc                                                                                                          >  2:131325/1‑136 (MQ=255)                                  aTGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTg                                                                                                     <  1:236972/136‑1 (MQ=255)                                  aTGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCGGGTAGGCGATGGACTCCAGTg                                                                                                     >  7:185086/1‑136 (MQ=255)                                     tCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTgcgg                                                                                                  <  1:314759/136‑1 (MQ=255)                                     tCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGCCGATGGACTCCAGTgcg                                                                                                   <  5:312190/135‑1 (MQ=255)                                      cGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTgcggc                                                                                                 <  1:94085/136‑1 (MQ=255)                                      cGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTgcggc                                                                                                 >  4:156508/1‑136 (MQ=255)                                        gCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGgcgc                                                                                               >  6:266118/1‑136 (MQ=255)                                        gCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGgcgc                                                                                               <  5:171843/136‑1 (MQ=255)                                        gCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGgcgc                                                                                               >  8:2633/1‑136 (MQ=255)                                          cTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCgg                                                                                             <  8:18342/136‑1 (MQ=255)                                             gAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGga                                                                                            <  1:330254/134‑1 (MQ=255)                                             gAACTGCATGAGGAAGATGTTGGCCACGGCACCTCCGTACACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGGCTGGTAGGCGATGGACGCCAGTGCGGCGCGgatg                                                                                          <  8:363751/136‑1 (MQ=255)                                                      gAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGTATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTc                                                                                  >  7:226460/1‑135 (MQ=255)                                                      gAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCAGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTc                                                                                  >  7:330351/1‑135 (MQ=255)                                                      gAGGAAGTTGTTGGCCACGGCACAACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACACTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGTCGCGGATGATGTGGTCt                                                                                 >  2:281535/1‑136 (MQ=255)                                                          aaGTTGTTGGCCACGGCACCACCGTACACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGAGATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCTGATGATGTGGTCTACCt                                                                             <  1:152383/136‑1 (MQ=255)                                                              tgttgGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCAc                                                                         >  6:325019/1‑136 (MQ=255)                                                               gttgGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCAc                                                                         <  7:44255/135‑1 (MQ=255)                                                               gttgGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCAc                                                                         <  3:340091/135‑1 (MQ=255)                                                               gttgGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCAc                                                                         <  1:82987/135‑1 (MQ=255)                                                                ttgGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGc                                                                       >  1:294335/1‑136 (MQ=255)                                                                   gCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCAc                                                                    <  2:179066/136‑1 (MQ=255)                                                                          caccacCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTGCCTTCACGCCACGTGTCAg                                                             >  5:330014/1‑136 (MQ=255)                                                                                cGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGaa                                                       <  5:263863/136‑1 (MQ=255)                                                                                    cACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCCGGCCGaacaac                                                   >  3:232067/1‑136 (MQ=255)                                                                                     aCGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACg                                                  >  4:169517/1‑136 (MQ=255)                                                                                     aCGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACg                                                  >  5:104851/1‑136 (MQ=255)                                                                                     aCGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCAGGTGTCAGGCCGAACAACg                                                  >  6:322119/1‑136 (MQ=255)                                                                                     aCGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGGCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACg                                                  <  5:187051/136‑1 (MQ=255)                                                                                       gcgcAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGca                                                <  5:266118/136‑1 (MQ=255)                                                                                       gcgcAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGca                                                <  6:290638/136‑1 (MQ=255)                                                                                       gcgcAGCTCGGACAGGCGCAGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGca                                                <  5:309650/136‑1 (MQ=255)                                                                                               cGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACg                                                                        <  6:26143/104‑1 (MQ=255)                                                                                               cGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACg                                                                        >  5:26143/1‑104 (MQ=255)                                                                                                ggACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCCCGTGTCAGGCCGAACAACGCCCCACGGGCa                                       >  3:52973/1‑136 (MQ=255)                                                                                                ggACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGc                                        <  7:240303/135‑1 (MQ=255)                                                                                                 gACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCAt                                      <  6:335261/136‑1 (MQ=255)                                                                                                     ggCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAg                                                                                                                     >  5:100562/1‑53 (MQ=255)                                                                                                     ggCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAg                                                                                                                     <  6:100562/53‑1 (MQ=255)                                                                                                      gcgcTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACggg                                 <  8:330351/136‑1 (MQ=255)                                                                                                      gcgcTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACggg                                 <  3:19407/136‑1 (MQ=255)                                                                                                      gcgcTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACggg                                 <  1:99624/136‑1 (MQ=255)                                                                                                          tGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCCCGGGCATACGGGTcc                              >  8:359583/1‑135 (MQ=255)                                                                                                          tGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCAGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTcc                              >  2:276734/1‑135 (MQ=255)                                                                                                                   tCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACgggg                    >  6:139841/1‑136 (MQ=255)                                                                                                                     ctgctgCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGc                   <  5:198332/135‑1 (MQ=255)                                                                                                                     ctgctgCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGc                   <  3:350121/135‑1 (MQ=255)                                                                                                                        ctgcATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCAccc               <  5:371599/136‑1 (MQ=255)                                                                                                                         tgcATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCAcc                >  1:7975/1‑134 (MQ=255)                                                                                                                              ggCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCg         >  3:15350/1‑136 (MQ=255)                                                                                                                                cGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCgg        >  4:379463/1‑135 (MQ=255)                                                                                                                                cGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCgg        >  8:298283/1‑135 (MQ=255)                                                                                                                                  tCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCGGTa      >  5:289475/1‑135 (MQ=255)                                                                                                                                   cGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCGGTa      >  5:366074/1‑134 (MQ=255)                                                                                                                                     aGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCGGTAAAgg  >  3:55018/1‑136 (MQ=255)                                                                                                                                        |                                                                                                                                     CGCATCTGCGGCCGCTCCACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCGGTAAAGG  >  ACB143/1227038‑1227305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑