Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,312,593 A→G 100% Q255R (CAG→CGG)  aaeA_2 → p‑hydroxybenzoic acid efflux pump subunit AaeA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,312,5930AG100.0% 288.1 / NA 80Q255R (CAG→CGG) aaeA_2p‑hydroxybenzoic acid efflux pump subunit AaeA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/33);  total (47/33)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCAGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAACAGGCAG  >  ACB143/1312460‑1312720
                                                                                                                                     |                                                                                                                               
cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGCCCTTCCACTGGGTGCGACTGGCCCGgc                                                                                                                               >  2:159073/1‑136 (MQ=255)
cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgc                                                                                                                               >  2:159071/1‑136 (MQ=255)
cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgc                                                                                                                               >  3:222261/1‑136 (MQ=255)
cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgc                                                                                                                               >  8:143166/1‑136 (MQ=255)
cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgc                                                                                                                               <  2:354143/136‑1 (MQ=255)
cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCAAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgc                                                                                                                               >  1:89607/1‑136 (MQ=255)
  gATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgcg                                                                                                                              >  5:64867/1‑135 (MQ=255)
  gATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgcg                                                                                                                              >  1:186887/1‑135 (MQ=255)
  gATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgcg                                                                                                                              >  1:210534/1‑135 (MQ=255)
       gCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATAcc                                                                                                                        <  1:161912/136‑1 (MQ=255)
             gTGACCCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAg                                                                                                                  <  1:198297/136‑1 (MQ=255)
             gTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAg                                                                                                                  >  6:54141/1‑136 (MQ=255)
             gTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAg                                                                                                                  <  7:101555/136‑1 (MQ=255)
                 aCCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGAtt                                                                                                              >  3:224935/1‑136 (MQ=255)
                  ccGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTc                                                                                                             <  2:376814/136‑1 (MQ=255)
                    gATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGc                                                                                                           >  1:163517/1‑136 (MQ=255)
                        cAGGGGCACGTGGCTAGCCTCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGGGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTgg                                                                                                       <  7:330043/136‑1 (MQ=255)
                        cAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTg                                                                                                        >  2:24286/1‑135 (MQ=255)
                        cAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTg                                                                                                        >  3:61355/1‑135 (MQ=255)
                        cAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTg                                                                                                        >  8:96850/1‑135 (MQ=255)
                           gggCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATc                                                                                                    <  4:316209/136‑1 (MQ=255)
                           gggCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATc                                                                                                    <  8:155091/136‑1 (MQ=255)
                           gggCACGGGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCGTCCGGAGGTAGAGCCGACCTTCATCTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATc                                                                                                    <  4:140430/136‑1 (MQ=255)
                            ggCACGTGGAGAGCATCAGCCGCTGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCa                                                                                                   <  2:49500/136‑1 (MQ=255)
                              cACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGCCTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAgg                                                                                                 >  1:81627/1‑136 (MQ=255)
                              cACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAgg                                                                                                 >  3:280591/1‑136 (MQ=255)
                                cGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTg                                                                                               >  5:38870/1‑136 (MQ=255)
                                  aggAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGcc                                                                                             <  3:7382/135‑1 (MQ=255)
                                   ggAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGTGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATAATGTGCCg                                                                                            >  6:110351/1‑136 (MQ=255)
                                   ggAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCg                                                                                            >  2:100362/1‑136 (MQ=255)
                                      gagCATCAGCCGCGTCATCACCGAGCGCAACAGCAACCCGGACAGCCAGTTGCTTCTGGATGTAGAGCCGACCTTCAACTGTGAGCGACTGGCCCGGCGCATACAGGTGAGGATTCGCATGGCTCAGGTGCCGGaa                                                                                         <  6:283808/136‑1 (MQ=255)
                                      gagCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGaa                                                                                         <  1:190001/136‑1 (MQ=255)
                                        gCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAgg                                                                                       <  1:141602/136‑1 (MQ=255)
                                          aTCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGCCCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGc                                                                                     >  4:21446/1‑136 (MQ=255)
                                          aTCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGc                                                                                     >  6:87629/1‑136 (MQ=255)
                                          aTCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGc                                                                                     >  3:366340/1‑136 (MQ=255)
                                          aTCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGc                                                                                     >  6:32825/1‑136 (MQ=255)
                                            cAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCt                                                                                    >  3:71749/1‑135 (MQ=255)
                                              gCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGGGCCGGAAGGGCTGAc                                                                                 <  7:138336/136‑1 (MQ=255)
                                               ccGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGAcc                                                                                >  6:279983/1‑136 (MQ=255)
                                               ccGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGAcc                                                                                >  1:141174/1‑136 (MQ=255)
                                               ccGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGAcc                                                                                >  2:16259/1‑136 (MQ=255)
                                                     cATCACCGACCGCCACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCCCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGt                                                                          >  7:268322/1‑136 (MQ=255)
                                                         accgaccgCAAGAGCAACCCGTACAGCCTGTTGCGGGCGGAGGTAGAGCCGAACTTCAACTTGGTGCGACTGGCCCGGCGCTTTCCGGTGAGGATTCGCCTGTATCTGGTGGGGGAATGGCTGACCTTGAGTGCg                                                                       <  1:353575/135‑1 (MQ=255)
                                                                gCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGAc                                                               <  4:46470/136‑1 (MQ=255)
                                                                gCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGAc                                                               >  7:56657/1‑136 (MQ=255)
                                                                  aaCAGCAACCCGGACAGCCAGTTTCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACgg                                                             <  7:143166/136‑1 (MQ=255)
                                                                    cAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCg                                                           >  2:209408/1‑136 (MQ=255)
                                                                     agaaaCCAGGACAGCCAGTTGCTGCCTGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTTACATTGAGTGCGGGGATGACgcc                                                            >  4:267888/4‑132 (MQ=38)
                                                                         aCCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGCGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAgtgt                                                      <  6:233221/136‑1 (MQ=255)
                                                                           ccGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGc                                                    <  1:89204/136‑1 (MQ=255)
                                                                           ccGGACAGCCAGCTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGc                                                    <  5:11792/136‑1 (MQ=255)
                                                                               aCAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGt                                                >  2:252830/1‑136 (MQ=255)
                                                                                  gCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCa                                             >  5:54948/1‑136 (MQ=255)
                                                                                     aGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGa                                          <  3:45111/136‑1 (MQ=255)
                                                                                          ctgcCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGAcc                                     <  2:195673/136‑1 (MQ=255)
                                                                                          ctgcCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGAcc                                     <  6:5954/136‑1 (MQ=255)
                                                                                                gAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGc                               >  6:251060/1‑136 (MQ=255)
                                                                                                 aGGTAGAGCCGACCTTCTACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGcc                              <  4:71749/136‑1 (MQ=255)
                                                                                                 aGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGcc                              <  1:12042/136‑1 (MQ=255)
                                                                                                      gagCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCTGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAg                          <  5:128350/135‑1 (MQ=255)
                                                                                                      gagCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAg                          <  8:210709/135‑1 (MQ=255)
                                                                                                          cGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGaag                     >  5:131755/1‑134 (MQ=255)
                                                                                                          cGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATg                     >  4:258059/1‑136 (MQ=255)
                                                                                                          cGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATg                     <  4:224935/136‑1 (MQ=255)
                                                                                                          cGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATg                     >  6:67392/1‑136 (MQ=255)
                                                                                                          cGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATg                     <  5:373600/136‑1 (MQ=255)
                                                                                                               ttCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGCGCCGCAGTGATGGTGCt                >  8:265518/1‑136 (MQ=255)
                                                                                                               ttCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCt                >  7:82064/1‑136 (MQ=255)
                                                                                                               ttCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCt                >  2:112363/1‑136 (MQ=255)
                                                                                                                tCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTg               >  4:179686/1‑136 (MQ=255)
                                                                                                                    cTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCt            >  8:237916/1‑135 (MQ=255)
                                                                                                                    cTGGGGTCGACTGGCCAGGCGCATACCTGTGATGATTCGCCTGGATCAGGTGCCGGAATGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCtt           <  7:145467/136‑1 (MQ=255)
                                                                                                                      gggTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTaa         >  3:260558/1‑136 (MQ=255)
                                                                                                                       ggTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAAc        <  1:24286/136‑1 (MQ=255)
                                                                                                                          gCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAACAgg     >  5:6598/1‑136 (MQ=255)
                                                                                                                           cGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAACAgg     >  7:155672/1‑135 (MQ=255)
                                                                                                                           cGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAACAGGc    <  6:106538/136‑1 (MQ=255)
                                                                                                                            gACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGCCCGCGAGCCGCAGTGATGGTGCTGGCTTAACAgg     >  3:33639/1‑134 (MQ=255)
                                                                                                                              cTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAACAGGCAg  >  3:142329/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                               
CTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCAGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAACAGGCAG  >  ACB143/1312460‑1312720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: