Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,346,999 A→G 100% R477R (CGT→CGC)  CJ019_01261 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,346,9990AG100.0% 283.4 / NA 74R477R (CGT→CGC) CJ019_01261hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/33);  total (41/33)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGCCGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTACGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAACGGCGGTGTCGTT  >  ACB143/1346877‑1347130
                                                                                                                          |                                                                                                                                   
ttGCCGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGtcct                                                                                                                        >  4:159314/1‑134 (MQ=255)
 tGCCGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGtcat                                                                                                                        <  2:179434/135‑1 (MQ=255)
  gCCGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGtcat                                                                                                                        <  6:69320/134‑1 (MQ=255)
  gCCGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGtcat                                                                                                                        <  7:219569/134‑1 (MQ=255)
   ccGGTCAGGTCGTGCATGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCAc                                                                                                                     <  5:191961/136‑1 (MQ=255)
   ccGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGCCGGCGGTCATCAc                                                                                                                     >  6:234695/1‑136 (MQ=255)
   ccGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCAc                                                                                                                     >  6:274440/1‑136 (MQ=255)
   ccGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCAc                                                                                                                     >  1:364524/1‑136 (MQ=255)
   ccGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCAc                                                                                                                     >  4:342271/1‑136 (MQ=255)
   ccGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCAc                                                                                                                     >  8:116847/1‑136 (MQ=255)
   ccGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCAc                                                                                                                     <  4:352808/136‑1 (MQ=255)
   ccGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTAATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGCAGGCGGTCATCAc                                                                                                                     >  6:218764/1‑136 (MQ=255)
   ccGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCGTCATGAACAGGTCAGGGATCCAGTTGGCGGTGATCATGTCGTGGGTGCGACGGCGGTCATCAc                                                                                                                     <  6:167867/136‑1 (MQ=255)
        cAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGgtg                                                                                                                <  3:47774/136‑1 (MQ=255)
          ggTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGtt                                                                                                              >  1:59342/1‑136 (MQ=255)
             cGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGGCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCtt                                                                                                           <  2:340666/136‑1 (MQ=255)
                    gTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGc                                                                                                    <  1:189027/136‑1 (MQ=255)
                        ggCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATAACCGGTGTTATTTCGCAGCTCGa                                                                                                >  2:387401/1‑136 (MQ=255)
                         gCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTc                                                                                                  <  3:276404/133‑1 (MQ=255)
                            cTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGCTGaa                                                                                            >  3:20498/1‑136 (MQ=255)
                            cTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACACGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGaa                                                                                            >  8:278048/1‑136 (MQ=255)
                               ccGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAAtt                                                                                          <  1:195857/135‑1 (MQ=255)
                                 gAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGGTCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAAttctt                                                                                       >  6:126482/1‑136 (MQ=255)
                                 gAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAAttgtt                                                                                       >  5:258132/1‑136 (MQ=255)
                                 gAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAAttctt                                                                                       >  7:265192/1‑136 (MQ=255)
                                 gAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAAttctt                                                                                       >  3:159740/1‑136 (MQ=255)
                                 gAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAAttctt                                                                                       >  4:292611/1‑136 (MQ=255)
                                  aaGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAAttcttc                                                                                      >  3:347919/1‑136 (MQ=255)
                                          aaCAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTc                                                                                >  3:208504/1‑134 (MQ=255)
                                           aCAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAg                                                                             >  5:73116/1‑136 (MQ=255)
                                                         ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                                <  7:117950/135‑1 (MQ=255)
                                                         ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                                <  1:408846/135‑1 (MQ=255)
                                                         ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                                <  8:229429/135‑1 (MQ=255)
                                                         ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                                <  5:274440/135‑1 (MQ=255)
                                                         ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                                <  5:65815/135‑1 (MQ=255)
                                                         ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGAGCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                                <  8:205497/135‑1 (MQ=255)
                                                          cGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAg                                                              >  3:245017/1‑136 (MQ=255)
                                                           gTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAgg                                                             <  6:168571/136‑1 (MQ=255)
                                                            tCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAgg                                                             >  6:289827/1‑135 (MQ=255)
                                                            tCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAgg                                                             >  7:122069/1‑135 (MQ=255)
                                                                cGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                                >  8:42849/1‑128 (MQ=255)
                                                                cGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                                <  7:42849/128‑1 (MQ=255)
                                                                 gAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCCCCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGca                                                       >  6:361621/1‑136 (MQ=255)
                                                                 gAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGc                                                        <  7:261376/135‑1 (MQ=255)
                                                                  aaGAGACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGcac                                                      <  3:270582/136‑1 (MQ=255)
                                                                     acacGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATa                                                   <  1:387401/136‑1 (MQ=255)
                                                                                 aaCAGGTCAGGGATCCAGTTGGCGGTGATCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCCATCTCCAGCTGCCAGGTTTCCAGGTAGGCACATACAGCCACTTTgc                                       >  2:374582/1‑136 (MQ=255)
                                                                                   cAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTgcgc                                     <  1:274179/136‑1 (MQ=255)
                                                                                        cAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGcc                                >  6:93718/1‑136 (MQ=255)
                                                                                        cAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGc                                 >  1:56117/1‑135 (MQ=255)
                                                                                            gATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCAccc                            >  3:246724/1‑136 (MQ=255)
                                                                                              tCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTg                          <  8:339678/136‑1 (MQ=255)
                                                                                                 aGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGgtg                                                                                                                >  5:234919/1‑47 (MQ=255)
                                                                                                 aGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGgtg                                                                                                                <  6:234919/47‑1 (MQ=255)
                                                                                                 aGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAg                                                          >  6:228900/1‑101 (MQ=255)
                                                                                                 aGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAg                                                          <  5:228900/101‑1 (MQ=255)
                                                                                                   ttGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACCGCGCCTTTGCGCTTGCCACCCTGGTTGa                     >  4:207503/1‑136 (MQ=255)
                                                                                                   ttGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGa                     >  6:275395/1‑136 (MQ=255)
                                                                                                   ttGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGa                     >  1:279064/1‑136 (MQ=255)
                                                                                                        ggTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGc                 >  3:148716/1‑135 (MQ=255)
                                                                                                        ggTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGc                 >  7:156040/1‑135 (MQ=255)
                                                                                                         gtgtTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCaa               <  5:274878/136‑1 (MQ=255)
                                                                                                           gtTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAAcg             <  6:303597/136‑1 (MQ=255)
                                                                                                            ttCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATTTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAAcgg            <  7:278048/136‑1 (MQ=255)
                                                                                                            ttCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAAcg             <  3:149932/135‑1 (MQ=255)
                                                                                                              cATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACAGCAAcggcg          >  2:147232/1‑136 (MQ=255)
                                                                                                               aTGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAAcggcgg         >  2:267752/1‑136 (MQ=255)
                                                                                                               aTGTCGTGGGTGCCACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGAAGTCCAGGTGCCACTTTTCCAGGAAGGCACATACACCGCCTTTGCCCTTGCCACCCTGGTTGACCGCAAcgccgg         >  2:273517/1‑136 (MQ=255)
                                                                                                                    gTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCACCGGCGGTGTCg    >  4:65529/1‑136 (MQ=255)
                                                                                                                     tGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAACGGCGGTGTCGt   <  7:300012/136‑1 (MQ=255)
                                                                                                                      gggTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAACGGCGGTGTCGtt  <  2:19653/136‑1 (MQ=255)
                                                                                                                       ggTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAACGGCGGTGTCGtt  >  7:303609/1‑135 (MQ=255)
                                                                                                                       ggTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAACGGCGGTGTCGtt  >  4:53386/1‑135 (MQ=255)
                                                                                                                       ggTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAACGGCGGTGTCGtt  <  5:231298/135‑1 (MQ=255)
                                                                                                                          |                                                                                                                                   
TTGCCGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTACGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAACGGCGGTGTCGTT  >  ACB143/1346877‑1347130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑