Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,986,661 A→C 44.5% intergenic (+42/+56) mshA_1 → / ← CJ019_01891 D‑inositol‑3‑phosphate glycosyltransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,986,6610AC44.5% 10.9 / 6.1 37intergenic (+42/+56)mshA_1/CJ019_01891D‑inositol‑3‑phosphate glycosyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (17/3);  new base C (12/5);  total (29/8)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAGTGAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCAGCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTGGGTCAA  >  ACB143/1986528‑1986794
                                                                                                                                      |                                                                                                                                      
ggAGTGAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGcgccgc                                                                                                                                      >  5:166456/1‑136 (MQ=255)
     gAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCATTT‑CGGCGCAGCCGCa                                                                                                                                  >  7:67130/1‑135 (MQ=255)
     gAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑AGGCGCAGCCGCAc                                                                                                                                 >  3:383863/1‑136 (MQ=255)
     gAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCACTG‑CGGCGCCGCCGCAc                                                                                                                                 >  3:370241/1‑136 (MQ=255)
         gTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACACTGCAGTG‑CGGCGCAGCCGCACTg                                                                                                                               >  8:264351/1‑134 (MQ=255)
              gCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCACTG‑CGGCGCAGCCCCACAGCA‑ggggg                                                                                                                       >  4:200877/1‑134 (MQ=255)
                       aaaCGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCAGCCCCACTGGC‑GGGGGTTTTCCata                                                                                                              >  5:286630/1‑136 (MQ=255)
                               ggTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCCGCCGCACTGCA‑GGGCTTTTGCCATATGACTTGt                                                                                                      >  8:201393/1‑136 (MQ=255)
                                     ggATTTTACGCAAAAAACTCCGCTCACCAATACGGCGCCTGCACACGCGCACTTATTGCTTACGCTGGGCCACCCATCACCCCTGCAGTG‑CGGCGCCGCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAg                                                                                                 <  2:28627/135‑1 (MQ=255)
                                              ggAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCCGCCCCCCGGCC‑GGGCGTTTGCCATATGACTTGTATGAGGCACGCAGt                                                                                        >  4:258436/1‑135 (MQ=255)
                                                aaaaTACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTT‑CGGCCCACCCGCCCTGGACGGGCTTTTG‑CAAATGACATTTTTGAGGCACGCAGtta                                                                                      >  2:168230/1‑133 (MQ=255)
                                                           ctcacccATACGGCCCCTGCCCACGCGCACTGTTGGCGTCCGCTGGCCCACCCAGCACCCCGGCAGGG‑CGGCCCCCCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATcc                                                                          <  2:286653/130‑1 (MQ=255)
                                                                        cgcCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCCGCCGCCCTGCA‑GGGCTTTTGCCATATGACTTGTTTGAGGCCCCGAGTAAACGCGCTTATACGGGTACGGGca                                                               >  3:397832/1‑134 (MQ=255)
                                                                        cgcCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCCGCCGCCCTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGcaca                                                             >  2:14533/1‑136 (MQ=255)
                                                                        cgcCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCCGCCGCACTGCA‑TGGGTTTTGGCATATGACGTGTTTGAGGCACGCAGTAAACGCGCGTATACGGGGACGGGcacc                                                             >  8:4827/1‑135 (MQ=255)
                                                                        cgcCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCAGCCGCCCTGCA‑GGGGTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGccca                                                             >  1:298401/1‑136 (MQ=255)
                                                                        cgcCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCAGCCGCACTGCA‑GGGCGTTTGCCATATGACTTGTATGAGGCCCGCAGTAAACGCGCTTATCCGGCTACGGGcaca                                                             >  7:47497/1‑136 (MQ=255)
                                                                        cgcCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCACCCGCACTGCA‑CGGCTTTTTCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTTTCCGGCTACGGGcaca                                                             >  8:8284/1‑136 (MQ=255)
                                                                            tGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCCGTG‑CGGCGCCGCCGCCCTGCA‑GGGGTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCGTATCCGGCTACGGGCCCAGCCt                                                         >  7:3988/1‑136 (MQ=255)
                                                                             gCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCAGCCGCACTGCC‑GGGCTTTTGCCATATGACTTGTATGAGGCCCGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTg                                                        >  7:82632/1‑136 (MQ=255)
                                                                              cTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCCGCCGCCCGGCA‑GGGGGTTTGGCATATGACGTGTATGGGGCCCGCAGTAAACGCGGGTATCCGGCTACGGGCCCAGCCggg                                                       >  5:146583/1‑136 (MQ=255)
                                                                              cTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCCGCCGCACTGCA‑GGGCTTTTGCCATATGACGTGTTTGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTgg                                                       >  1:263287/1‑136 (MQ=255)
                                                                              cTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCAGCCGCACTGCA‑GGGCGTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCggg                                                       >  1:319216/1‑136 (MQ=255)
                                                                              cTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CCGCGCCGCCGCCCTGCA‑GGGCGTTTGCCAGATTGCATGTATGAGGCCCGCAGTAAACGCGCTTATACGGCGACGGGCACCCCCTgg                                                       >  2:153912/1‑136 (MQ=255)
                                                                                cacgcgcACTTATTCCTAACGCTTGCCCATCAATCAACCCTG‑ACTGCCGGCCCAGCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCg                                                     <  3:303551/133‑1 (MQ=255)
                                                                                cacgcgcACTGATTGCGTACGCTTGCCCACCCATCACCCCGCCAGCG‑CGGCCCCCCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCg                                                     <  5:386837/133‑1 (MQ=255)
                                                                                                      ttGGCCATCCATGAACCCTGCAGTG‑CGGCGCAGCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGtt                                 >  2:244164/1‑134 (MQ=255)
                                                                                                         gCCATCCATGAACCCTGCAGTG‑CGGCGCCGCCGCACTGCA‑GGGGGTTTTGCATATGTCTTGTTTTAGGCACGCAGTAAACGCGCGTTTACGGCGACGGGCACCGCCTGGCGACGCGGGCGCAGATAGCGGTGAGcc                            >  4:356283/1‑136 (MQ=255)
                                                                                                         cccacccatCACCCCTCCCGGG‑CGCCGCCGCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGcc                            <  2:267736/135‑1 (MQ=255)
                                                                                                             cccatCACCCCTGCCGGC‑CGCCCCACCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGc                        <  1:232165/135‑1 (MQ=255)
                                                                                                                 tGAACCCTGCAGTG‑CGGCGCAGCCGCCCTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTc                    >  1:267736/1‑136 (MQ=255)
                                                                                                                 tGAACCCTGCAGTG‑CGGCGCAGCCGCAATGGA‑GGGCATTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCCCTTATACGGCTACGGGCAAAACCTGGCGAAGCTGGCGCAGATAGCGTTGAACCGGGCGtt                     >  8:130161/1‑135 (MQ=255)
                                                                                                                           aGTG‑CGGCGCAGCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTTTGAGGCACGCAGTTAACGCGCTTATACGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCgg          >  8:41050/1‑136 (MQ=255)
                                                                                                                           aGTG‑CGGCGCAGCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTAAGGGCACAGCCTGGCGCCGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCgg          >  6:198024/1‑136 (MQ=255)
                                                                                                                           aGTG‑CCGCGCCGCCGCCCGGCA‑GGGCTTTTTACATAAGACTTGTATGAGGCACGCAGTAAAACCGCTTAACCGGCAAAGGGCACACCCAGGCGCCGCGGGCGCCAGTATCGGTGCGCCCGGCGGTCcacccagccg          >  4:330271/1‑134 (MQ=255)
                                                                                                                                 gggccAGCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTGGGt     <  3:204561/132‑1 (MQ=255)
                                                                                                                                 ggcgCCGCGCCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTGGGt     <  3:200877/136‑1 (MQ=255)
                                                                                                                                   cgcAGCCGCACAGCA‑GGGCTTTTGCCATATTACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCAGGCGACGCAGGCGCAGATAGCGTTGAGCCGGGCGTTCCCGCCAGCGGTGGGTc    >  5:366563/1‑135 (MQ=255)
                                                                                                                                      aGCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTGGGTCaa  >  3:69864/1‑134 (MQ=255)
                                                                                                                                      aGCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTGGGTCaa  >  4:224140/1‑134 (MQ=255)
                                                                                                                                      |                                                                                                                                      
GGAGTGAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTG‑CGGCGCAGCCGCACTGCA‑GGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTGGGTCAA  >  ACB143/1986528‑1986794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑