Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,171,262 C→A 11.9% F147L (TTC→TTA CJ019_02060 → IS1182 family transposase ISPpu16

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,171,2620CA11.9% 265.5 / 6.4 134F147L (TTC→TTACJ019_02060IS1182 family transposase ISPpu16
Reads supporting (aligned to +/- strand):  ref base C (74/42);  new base A (2/16);  total (76/58)
Fisher's exact test for biased strand distribution p-value = 4.02e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GGATTTCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGC  >  ACB143/2171127‑2171393
                                                                                                                                          |                                                                                                                                     
  atctCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCa                                                                                                                                      <  6:67384/132‑1 (MQ=255)
   tttCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCaaa                                                                                                                                    <  7:212678/136‑1 (MQ=255)
                 ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTTCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATcg                                                                                                                    >  3:163363/1‑136 (MQ=255)
                 ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATcg                                                                                                                    >  8:9148/1‑136 (MQ=255)
                 ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCAGAGTCGATCATTGCCATCGACGGCAGCAAGGTCAAAGCAGTGCACAATcg                                                                                                                    >  7:339428/1‑136 (MQ=255)
                         cGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGt                                                                                                             >  1:172810/1‑135 (MQ=255)
                            aaGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACt                                                                                                         >  7:198142/1‑136 (MQ=255)
                              ggACAACGGTAAAGCCATTGGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTTAACAATCGCGACCGTAACTTc                                                                                                       <  7:386734/136‑1 (MQ=255)
                              ggAAAACGTTAAAGCCATTCGTAGCGTCTGCCGTCAGTTGGTGATCCTCTGCCTTAACCTCAACCTTTTCTCTTTGTCTATCATTGCCATCGACGGCAGCAATTTCAAAGCAGTGAACAATCGCGACCGTAACTTc                                                                                                       <  8:204632/136‑1 (MQ=255)
                                     gttAAAGCCATTCGTCGCGTCTGCCGTCTGTTCGTGAGCCTGTGCCTTAACCTCAACCTTTTCTCTGTGTCCATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAgg                                                                                                <  7:298512/134‑1 (MQ=255)
                                     ggaaaaGCAATTCGTCGCGTCTGCCGTCTGTTCGTGATCCTCTGCCGAAACCTAAACATTTTCTCTGTGTCGATCATTGCCATCGACGGCAGCAAGTTAAAAGCAGTCAAAAACCGCGACCGTAACTTCACCCAgg                                                                                                <  8:1547/133‑1 (MQ=255)
                                     ggTAAAGCCATTCGTAGCGTCTGCCGTCTGTTCTTTACCATATGCCGAAACCTCAACCTTTTCTCTGCTGCGATAATTGCCATCGCCGGCAGCACGTTAAAAGCTTTTAAAAATCGCGACCGTAACTTCACCCAgg                                                                                                <  2:94490/136‑1 (MQ=255)
                                     ggTAAAGCAATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTCAACAATCGCGACCGTAACTTCACCCAgg                                                                                                <  7:382812/136‑1 (MQ=255)
                                          acccATTCGTCGCGGCTGCCGGCAGTGCGGCAGCCTCTGCCGAACCCTCAACCTTTGCTCTGAGGCTATCATGCCCACCGCCGGCAGCATTTACAACGCCGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAg                                                                                           <  8:17820/134‑1 (MQ=255)
                                          aGCCATTCGTCGGGTGTGCCGGCTGTTCGTGATCCTCTGCCTAAACCTCAACATTTTTTCTGAGTCCAACATTCCCATCGACGGCAGAAAGTACAAACCTGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAg                                                                                           <  2:141026/136‑1 (MQ=255)
                                           gCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTAAAAGCATTGAAAAATCGAGACCGTAACTTCACCCAGGCCAAgg                                                                                          >  5:64226/1‑136 (MQ=255)
                                            ccATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                         >  1:287091/1‑136 (MQ=255)
                                             cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTTCCATCGACGGCAGCAAGTACAAACCAGTTAACAATCGCGAACGTAACTACCCCCAGGCCAAggg                                                                                         >  6:127042/1‑134 (MQ=255)
                                             cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGTACAATCGCGACCGTAACTTCACCCAGGCCAAGGTg                                                                                        >  2:381102/1‑136 (MQ=255)
                                             cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                         >  4:189149/1‑135 (MQ=255)
                                               ttCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCCGCAAGTTCAAAGCAGTGAACAATAGCGACCGTAACTTCACCCAGGCCAAGGTg                                                                                        >  6:61750/1‑134 (MQ=255)
                                                 cGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAgg                                                                                    >  7:308721/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTACCCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGAAGTGAACAATCGCGACCGTATCTTCACCCAGGCCAAGGTGAAGGc                                                                                   >  6:361580/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTTCCATCGACGGCAGCAACTTCAAAGCAGTGAACAATCGCTACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                   >  7:28842/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                   >  8:371150/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                   >  6:137885/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAAATTCACACAGGCCAAGGTGAAGGc                                                                                   >  6:307106/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAAAAATCGCGACCGTAACTTCACCCAGCCCAAGGTGAAGGc                                                                                   >  6:353421/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAACTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGACAAGGTGAAGGc                                                                                   >  4:139861/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCAGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATAGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                   >  2:67263/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTACGTGATCCTCTGCCGTAACCACAACCTTTTCTCTGAGTCGATCATTGCCAACGACGGCCGCAAGTTCACAGCAGAGAACAATCGCGACCGTAACTTCCACCACGCCAAGGTGCAGGc                                                                                   >  4:230278/1‑136 (MQ=255)
                                                        tCTGCCGTCTGTTCGTGATCTCCTGCCGAAACCACAACCTTTTCTGTGAGTCTATCATTGCCAGCGACGGCAGAAAGTACAACGCCGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCAt                                                                             <  2:172810/136‑1 (MQ=255)
                                                         cTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                            >  3:245150/1‑136 (MQ=255)
                                                         cTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                            >  2:72223/1‑136 (MQ=255)
                                                          tGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                            >  3:369651/1‑135 (MQ=255)
                                                           gCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGCCGGCAGCAAGTTCAAAGCAGTGAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                            >  7:150202/1‑134 (MQ=255)
                                                           gCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGCAAAATCGCGACCGTAACTTCAACCAGGCCAAGGGGCAGGCCCGCATggc                                                                          >  2:208589/1‑134 (MQ=255)
                                                             cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                         >  6:397775/1‑135 (MQ=255)
                                                               acAGTTCGTGACCCTCTGCCGAACCCACAACATTTTCTCTGAGCCGAAAATTCCCATCGACGGCAGCAAGTGAAAAGCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCaa                                                                      <  5:62223/135‑1 (MQ=255)
                                                                   ttCGTTATCCTCTGCCTAAACCAAAACTTTTTCTCTGAGTCTATAATTGCCAGCGAGGGCAGCAAGTAAAAAGCTGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                  <  8:82901/136‑1 (MQ=255)
                                                                    tgttGATCCTGTTCCTTAACTTAATNATTTTNTCTGTNTCTTACATTCCATTCGAGGGAAGCATTTTAAAACCTTTAAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                  <  2:361344/132‑1 (MQ=255)
                                                                    tCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATc                                                                   >  5:396453/1‑134 (MQ=255)
                                                                    gcGTGAGCCTCTGCCGTAACCTCAACCTTTTCTCTGAGGCGATCATGGCCATCGACGGCAGCAAGTTCAACGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                  <  8:125500/134‑1 (MQ=255)
                                                                     ggTGATCCTCTGCCGTAACCTCAACCTTTTGTTTGTTTCTTTAATTGCCTTTGAGGTCAGAAATTTAAAACCATTTAAAAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                 <  4:153157/134‑1 (MQ=255)
                                                                     ctttaTCCTGTGCCGTACCCTCAACTTTTTCTCTGAGGGGATAATGTCCATCGACGGCATCATGTTCAAAGCAGTTAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                 <  8:298047/131‑1 (MQ=255)
                                                                     cGTGATCCTCTGCCGTAACATCAACCTTTTCTCTGAGTCTATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTTAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                 <  4:226778/135‑1 (MQ=255)
                                                                      tttaggctctGACGAAACCTAACCATTTTCTTGTCGTCTATCATTGCATCACCGGGCATAAATTTCAAAGCTGTAACAATCCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagc                                                               <  4:100778/130‑1 (MQ=255)
                                                                         acccTCTCCCGTAACCACACCGTTTTCTCGGCGCCAAGAATTCCCATCGACGGCAGAATTTAAAAACCATTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcaga                                                            <  3:340583/134‑1 (MQ=255)
                                                                          tCCTCTGCCGTAACCTCAACCTTTTCTCTGCGTCGATCATTTCCATCGCCGGCAGCAAGTTAAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgctcagag                                                           >  5:202367/1‑136 (MQ=255)
                                                                          tCCTCTGCCTAACACTCAACATTTTCTCTGAGTCTATAATTGCCATCGAGGGCAGCAAGTTCAAAGCAGTAAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcaga                                                            <  3:230424/135‑1 (MQ=255)
                                                                          accTCTGCCGTCACCACACCATATCATCTGAGCGGATCTTACCCATCCACGGCTCCAAGATCACAGCAGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagag                                                           <  2:384008/135‑1 (MQ=255)
                                                                           ccTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGAAAATCgagcagag                                                           >  3:284279/1‑135 (MQ=255)
                                                                           ccTCTCCCTAAACCACAACTTTTACTCTGTGTCCATAATTGCCATCGAGGGCAGCAATTTCAAACCAGTAAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagc                                                          <  4:370516/136‑1 (MQ=255)
                                                                           ccTCGGCGGAAACATCAACTTTTTCACGGAGTCCATCATTGCCATCGCCGGCAGCATTTCAAACCCAGGTAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCGGCATGCAGCAAATCgagcagagc                                                          <  7:182915/136‑1 (MQ=255)
                                                                                gcctaaaCCTCAACCTTTTATCTGCGCCCACCATTGCCATCTAGGGCAGCATGTTAAAAGCTTTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  6:333171/131‑1 (MQ=255)
                                                                                gccgacaCCTAAACCTTTTCCATCAGGCCATCATTCCCATCCAGGGCACCAAGTACAACCATGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  4:79656/130‑1 (MQ=255)
                                                                                gCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATAGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                       >  1:361344/1‑134 (MQ=255)
                                                                                gCCGTAACCTAAACTTTTTCTCTGAGCCGATATTTGCCATGCAGGGAAGAATTTTAAAAGCATTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  3:374438/136‑1 (MQ=255)
                                                                                gCCATACCCTCACCCTTTTCTCTCCGTCGAGAATTGCCAGCGAGGGCAGCATGTTCAACGCAGTCAACACCCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  6:360310/136‑1 (MQ=255)
                                                                                cccGTAACCCCAACATTTTCTCTGCGTCCTAAATGCCAAGCTCGGGCAGAAATTACAAACCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  4:201381/135‑1 (MQ=255)
                                                                                 ccttAACCTCAACTTTTTCTCTGAGTCTATCTTTCCCATCGACGCCAGAAATTCAAAACCAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  4:90643/132‑1 (MQ=255)
                                                                                    tAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCCCCCAGGCCAAGGTGAAGGCCCGCAGGCAGCAAATCGAGCAGAGCATTGAgcgc                                                 >  8:136169/1‑136 (MQ=255)
                                                                                       ccTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGCGCGCTAc                                              >  8:144184/1‑136 (MQ=255)
                                                                                       ccTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc                                              >  8:374225/1‑136 (MQ=255)
                                                                                       ccTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc                                              >  3:370020/1‑136 (MQ=255)
                                                                                         cTCAACTTTTTCTCTGAGTCGATCATTCCCATCGCCGGCAGCAATTTAAAAGCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc                                             <  5:116875/136‑1 (MQ=255)
                                                                                          tCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGCCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTAACCCAGGCCAAGGTGAAGGACCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCAAcc                                             >  6:37792/1‑135 (MQ=255)
                                                                                               cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTCCCTTACAg                                       >  1:22893/1‑136 (MQ=255)
                                                                                               cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                       >  2:390979/1‑136 (MQ=255)
                                                                                               cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                       >  3:187057/1‑136 (MQ=255)
                                                                                               cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                       >  3:201240/1‑136 (MQ=255)
                                                                                               cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                       >  7:62106/1‑136 (MQ=255)
                                                                                               cTTTTCTCTGAGTCGATCATAGCCATCGACGGCAGCAAGTTCAAAGCAGTGAAAAATCGCGACCGTAACTTCACCCAGGGCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                       >  3:65070/1‑136 (MQ=255)
                                                                                                    ctctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATg                                  >  5:322579/1‑136 (MQ=255)
                                                                                                     tctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATAGAGAGATACCTTACAGCGATg                                  >  8:222099/1‑135 (MQ=255)
                                                                                                      ctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGa                                >  1:353894/1‑136 (MQ=255)
                                                                                                        gatgctaTCATTCCCTTCCAGGGAAGCATGTACAAAGCCTTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGAt                               <  1:365592/129‑1 (MQ=255)
                                                                                                         aGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCACTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTc                             >  4:196411/1‑136 (MQ=255)
                                                                                                           tCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                           >  4:115007/1‑136 (MQ=255)
                                                                                                           tCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                           >  4:109155/1‑136 (MQ=255)
                                                                                                            cGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCTACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGAATTGAGCGCTACCTTACAGCGATGGATTCgg                           >  5:206721/1‑135 (MQ=255)
                                                                                                            cGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                           >  7:169418/1‑135 (MQ=255)
                                                                                                            cGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                           >  3:214174/1‑135 (MQ=255)
                                                                                                            cGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                          >  2:173300/1‑136 (MQ=255)
                                                                                                                 aTTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                     >  4:315913/1‑136 (MQ=255)
                                                                                                                 aTTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGAATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                     >  5:177696/1‑136 (MQ=255)
                                                                                                                  ttcccAGCGACGGCAGCAAGATCAAACCAGGGAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCGAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                    <  5:125315/133‑1 (MQ=255)
                                                                                                                  ttGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     >  6:93790/1‑103 (MQ=255)
                                                                                                                  ttGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  5:93790/103‑1 (MQ=255)
                                                                                                                   tcccATCGACGGCAGCATGTTCAAAGACGTCAAAAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                    <  2:238407/133‑1 (MQ=255)
                                                                                                                      cATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc                 >  7:55805/1‑135 (MQ=255)
                                                                                                                        aTCGACGGCAGCAAGTTCAAAGCAGTGAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCaa               <  8:37452/136‑1 (MQ=255)
                                                                                                                            aCGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGcc           >  5:211735/1‑136 (MQ=255)
                                                                                                                            aCGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGcc           >  6:168737/1‑136 (MQ=255)
                                                                                                                                gcagcaAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg          >  6:209519/1‑134 (MQ=255)
                                                                                                                                    caATTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg    <  8:261731/136‑1 (MQ=255)
                                                                                                                                    caAGTTCACAGCAGTCACAAAACGCGACCGTAACTTCACCCAGGCCAAGGTTAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg    <  8:146538/136‑1 (MQ=255)
                                                                                                                                    caAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg    >  7:322900/1‑136 (MQ=255)
                                                                                                                                     aaGTTAAAACCAGCCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg    <  5:148875/135‑1 (MQ=255)
                                                                                                                                       gTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGc  >  8:275206/1‑135 (MQ=255)
                                                                                                                                          |                                                                                                                                     
GGATTTCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGC  >  ACB143/2171127‑2171393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: