Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 3,052,174 T→C 7.4% L138P (CTG→CCG)  CJ019_02867 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,052,1740TC 7.4% 167.4 / 2.1 53L138P (CTG→CCG) CJ019_02867hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (14/35);  new base C (2/2);  total (16/37)
Fisher's exact test for biased strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCGATGCCTGGCTCGGCCGCCAGTTGTTCGGCGAGCGCTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAGCTGCTGGGGGCCTTGCACCAGGTCTCAGGCGGGCAATTGAC  >  ACB143/3052040‑3052309
                                                                                                                                      |                                                                                                                                       
tATCGATGCCTGGCTCGGCCGCCAGTTGTTCGGCGAGCGCTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTg                                                                                                                                        >  2:159948/1‑136 (MQ=255)
  tCGATGCCTGGCTCGGCCGCCAGTTGTTCGGCGAGCGCTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGca                                                                                                                                      <  7:280835/136‑1 (MQ=255)
        ccTGGCTAGGCCGCCAGTTGTTCGGCGAGCGCTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGAGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACt                                                                                                                                <  3:47494/136‑1 (MQ=255)
          tgtcTCGGCCGCGAGTTGTTCGGCGAGCGCTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGc                                                                                                                              <  1:35678/133‑1 (MQ=255)
           ggCTCGGCCGCCAGTTGTTCGGCGAGCGCTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCt                                                                                                                             <  3:105498/136‑1 (MQ=255)
            ggtCGGCCGCCAGTTGTTCGGCGAGCGCTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGc                                                                                                                              <  6:270839/132‑1 (MQ=255)
            gCTCGGCCGCGAGTTGTGCGGCGAGCGCTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCGGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTTGGAGTCCCTTAAAGAACTGc                                                                                                                              <  8:110190/134‑1 (MQ=255)
            gCTCGGCCGCCAGTTGTTCGGCGAGCGCTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGc                                                                                                                              <  8:64170/134‑1 (MQ=255)
             cTCGGCCGCCAGTTGTTCGGCGAGCGCTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTg                                                                                                                           >  6:16981/1‑136 (MQ=255)
                            ttCGGCGAGCGCTACCACAGCAGCCGGCGGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTg                                                                                                             <  8:29614/135‑1 (MQ=255)
                                  gAGCGCTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCgttgt                                                                                                      <  3:137204/136‑1 (MQ=255)
                                    gcgcTACCACCGCAGCCGGCTGCTGGACTTGAGTGAGGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGttgttg                                                                                                    <  8:263079/136‑1 (MQ=255)
                                    gcgcTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGttgttg                                                                                                    <  1:346762/136‑1 (MQ=255)
                                    gcgcTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGttgttg                                                                                                    <  7:316393/136‑1 (MQ=255)
                                    gcgcTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGttgttg                                                                                                    <  2:10185/136‑1 (MQ=255)
                                    gcgcTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGttgttg                                                                                                    <  5:343738/136‑1 (MQ=255)
                                    gcgcTACCACAGCAGCCGGCGGGTGGTCTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGttgttg                                                                                                    <  5:42413/136‑1 (MQ=255)
                                                     ggCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCAt                                                                                   >  1:81622/1‑136 (MQ=255)
                                                     ggCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCAt                                                                                   >  4:196708/1‑136 (MQ=255)
                                                     ggCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCAt                                                                                   >  1:398678/1‑136 (MQ=255)
                                                       ctgctgGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATcc                                                                                 <  1:11764/136‑1 (MQ=255)
                                                          ctgGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCt                                                                              <  3:400368/136‑1 (MQ=255)
                                                          ctgGACTTGAGTGAGGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCt                                                                              <  4:61061/136‑1 (MQ=255)
                                                                   aGTGCTGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTa                                                                      <  8:153837/135‑1 (MQ=255)
                                                                   aGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGTGATGACATCGATGAATTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTTGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCTGTa                                                                      <  8:26391/135‑1 (MQ=255)
                                                                   aGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTa                                                                      <  2:270246/135‑1 (MQ=255)
                                                                   aGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTAc                                                                     <  3:97700/136‑1 (MQ=255)
                                                                    gTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTAcg                                                                    <  1:51363/136‑1 (MQ=255)
                                                                    gTGATCGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTAcg                                                                    <  7:360871/136‑1 (MQ=255)
                                                                     tGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCCGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTAcgg                                                                   >  3:52397/1‑136 (MQ=255)
                                                                     tGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCCGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTAcgg                                                                   >  3:57958/1‑136 (MQ=255)
                                                                         ggCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTAcggcgg                                                                <  1:159948/135‑1 (MQ=255)
                                                                           ccgccgCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGca                                                                                                                                      <  3:307820/63‑1 (MQ=255)
                                                                           ccgccgCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGca                                                                                                                                      >  4:307820/1‑63 (MQ=255)
                                                                             gccgcGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCAt                                                           >  6:104198/1‑136 (MQ=255)
                                                                               cgcgTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATgg                                                         >  3:348813/1‑136 (MQ=255)
                                                                               cgcgTGTTGATGGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATgg                                                         <  7:167483/136‑1 (MQ=255)
                                                                                               tGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAgct                                         >  6:11734/1‑136 (MQ=255)
                                                                                                ggATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAgctg                                        >  4:152918/1‑136 (MQ=255)
                                                                                                 gATGCGCGGGATGAAATCGCTGAAGTCTGGGACTCCCCGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAgctgc                                       <  4:57958/136‑1 (MQ=255)
                                                                                                 gATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCCGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAgctgc                                       <  4:52397/136‑1 (MQ=255)
                                                                                                  aTGCGTTGGATGAAATCTATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAgctgct                                      <  2:22193/136‑1 (MQ=255)
                                                                                                       cgGGATGAAATCTATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGGTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCGGCTGGATGAGCTGCTggggg                                 <  6:29/136‑1 (MQ=255)
                                                                                                       cgGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAGCTGCTggggg                                 <  8:118545/136‑1 (MQ=255)
                                                                                                            tGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGGTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAGCTGCTGGGGGCCTTg                            <  6:361384/136‑1 (MQ=255)
                                                                                                             gtattCGTTGTAGTCTGGGACTCCCTGCATCAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAGCTGCTGGGGGCCTTg                            <  3:298370/131‑1 (MQ=255)
                                                                                                              aaaTCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAGCTGCTGGGGGCCTTGc                           <  7:317110/135‑1 (MQ=255)
                                                                                                                       aaGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGAt                                              >  8:319612/1‑107 (MQ=255)
                                                                                                                       aaGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGAt                                              <  7:319612/107‑1 (MQ=255)
                                                                                                                       aaGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAGCTGCTGGGGGCCTTGCACCAGGtct                  >  1:182642/1‑135 (MQ=255)
                                                                                                                             gggACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAGCTGCTGGGGGCCTTGCACCAGGTCTCAGGCgg           >  5:72932/1‑136 (MQ=255)
                                                                                                                              ggACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAGCTGCTGGGGGCCTTGCACCAGGTCTCAGGc             <  1:208788/133‑1 (MQ=255)
                                                                                                                                 cTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAGCTGCTGGGGGCCTTGCACCAGGTCTCAGGCGGGCaa       >  8:283367/1‑136 (MQ=255)
                                                                                                                                      tGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAGCTGCTGGGGGCCTTGCACCAGGTCTCAGGCGGGCAATTGAc  >  2:163710/1‑136 (MQ=255)
                                                                                                                                      |                                                                                                                                       
TATCGATGCCTGGCTCGGCCGCCAGTTGTTCGGCGAGCGCTACCACAGCAGCCGGCTGCTGGACTTGAGTGATGGCCGCCGCGTGTTGATTGCCGTGGATGCGCGGGATGAAATCGATGAAGTCTGGGACTCCCTGCAACAACTGCTTGGCGTGTGTGGCCTGGCGTTGTTGCTGAGCTTGTGGACCATCCGCTGGGCGGTACGGCGGGCCATGGGCCTGCTGGATGAGCTGCTGGGGGCCTTGCACCAGGTCTCAGGCGGGCAATTGAC  >  ACB143/3052040‑3052309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑