Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 3,427,762 T→G 24.4% F77V (TTC→GTC)  CJ019_03251 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,427,7620TG24.4% 105.8 / 2.8 55F77V (TTC→GTC) CJ019_03251hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (8/31);  new base G (13/2);  total (22/33)
Fisher's exact test for biased strand distribution p-value = 1.29e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACAAGTCACTTTCGCCGACACCGTCTACACCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGACCACTTGCGCCAATCCATCGAAGACATCTTGACCACGCCACT  >  ACB143/3427630‑3427894
                                                                                                                                    |                                                                                                                                    
gCACAAGTCACTTTCGCCGACACCGTCTACACCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTcc                                                                                                                                   <  6:85590/136‑1 (MQ=255)
gCACAAGTCACTTTCGCCGACACCGTCTACACCTCGCGCTCGCTGAACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTcc                                                                                                                                   <  8:70307/136‑1 (MQ=255)
    atgTCACTTTCCCCTACACCTTCTACACCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGATTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGc                                                                                                                               <  6:171248/134‑1 (MQ=255)
    aaGTCACTTTCGCCGACACCGTCTACACCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGc                                                                                                                               <  4:391595/136‑1 (MQ=255)
    aaGTCACTTTCGCCGACACCGACTACACCTCGCGCTCGCTGTACCTGGACACGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGc                                                                                                                               <  5:215494/136‑1 (MQ=255)
     aggcACTTTCGCCGACACCGTCTACACCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCTAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCt                                                                                                                              <  3:23702/133‑1 (MQ=255)
     aGTCACTTTCGCCGACACCGTCTACACCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCt                                                                                                                              <  2:76768/136‑1 (MQ=255)
       tCACTTTCGCCGACACCGTCTACACCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGa                                                                                                                            >  1:42723/1‑136 (MQ=255)
         aCTTTCGCCGACTCCGTCTACACCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATc                                                                                                                          <  2:131557/136‑1 (MQ=255)
         aCTTTCGCCGACACCGTCTACACCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATc                                                                                                                          <  1:124164/136‑1 (MQ=255)
         aCTTTCGCCGACACCGTCTACACCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATc                                                                                                                          <  2:148548/136‑1 (MQ=255)
         aCTTTCGCCCACACCTGCAACACCTCGCGCTCGCTGTACCTTGACTAGGGCGAGGAGCTGCGCGAGTTCAAGGGGGTGGCCATGCGCCTCTGCGTGGCGGGCGACGACGCCGAGGGTCGGGCCGTCCTGGCTGATc                                                                                                                          <  8:379066/136‑1 (MQ=255)
            tgcgcgGACACCGTCTACACGTCGCGCTCGCGTTCCCTGGACAAGGGCGAGGACCTGCGCGTGTTAACGGGGGGGGCCAAGCGCCTCAGGGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCAc                                                                                                                        <  4:6182/133‑1 (MQ=255)
                   acacCGTCTACACCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGCCGACCCCCCGGCGCTGGCCGTACTGGCTGATCACCCTGAGCt                                                                                                                >  2:37331/1‑136 (MQ=255)
                            acacCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCCAGCCCCTCAGCGTGCCGGCCGCCGCCCCCGAGGCGCGGGCCGTCCAGGGTGATCCCCCTGAGCTGCAGCGGCt                                                                                                       >  6:355547/1‑136 (MQ=255)
                            acacCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCt                                                                                                       >  8:320967/1‑136 (MQ=255)
                                   gcgcTCGCTGTCCCTGGACACGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACg                                                                                                  <  4:116243/134‑1 (MQ=255)
                                   gcgcTCGATGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGGCCAGCGCCTCCTCGTGCCCGCCGCCCCCGCCGCGGGGCTGGCCGTGCTGGGTGATCACCCGGGGCGGCAGCGGCTgggccgc                                                                                                >  7:208507/1‑131 (MQ=255)
                                    cgctcgctGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCt                                                                                               <  2:387993/136‑1 (MQ=255)
                                    cgctcgctGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCACCGCGTGCCGGCCGCCCGCGCCCCCGCGCTGGGCGTGCAGGGTGGTGCCCCAGAGCTGCCGCGGCGGggcggcg                                                                                               >  3:62442/1‑135 (MQ=255)
                                     gctcgctGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCACAGCGTGCCGGCCGCCGCCCCCCCGGCGCGGGCCGTGCGGGGTGGTGACCCGGAGCTGCAGCGGCGGGCCggggg                                                                                              >  4:100971/1‑133 (MQ=255)
                                     gctcgctGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCCCCACCGCCTGCCGGCCCCCGACGCCGAGGCGCGGGCCGGCCTGGCGGGTGACCCAGCGCTGGAGCCGCgggtccgcg                                                                                               >  4:101550/1‑129 (MQ=255)
                                     gctcgctGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAACCGCCGCAGCGTGCCGGCCGCCGCCGCCGACGCGCGGGCCGTCCTGGCGGCTAACCCTGAGCTGCAGCGGCTGggcggcgg                                                                                              >  5:326588/1‑134 (MQ=255)
                                     gctcgctGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAACCCCCGCACCGGGCCGGGCGCCGCCGCCGCCGCGCGGGCCGTGCTGGGTGATGACCCGGCGCGGGCGCGGGTGggcggcgg                                                                                              >  7:51079/1‑134 (MQ=255)
                                     gctcgctGTACCTGGACAAGGGCGAGGACCTGCGCGAGCTCAAGGTGGTGGCCAAGCCCCCCCGCGGGCCGGCCGCCGCCGCCGCGGCGCTGGCCGGGCGGGCTGATACCCCCGAGCTGCAGCCGCCGGACGGCTg                                                                                              >  3:242529/1‑136 (MQ=255)
                                      ctcgctGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCCAGCGCCGCAGCGTGCCCGCCCCCGCCGCCGCCGCCCGGGCCGTCCCGGGGGATCCCCCAGAGCTGCAGCCGCGGGCCGGcggc                                                                                             >  3:362843/1‑133 (MQ=255)
                                      ctcgctGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAACCGCCGCAGCGGGCCCGCCGCCCACCCCCCCGCGCATGCCATACTGGCGGGTCAACCGGAGCAGCAGCGGCAGGGCGGCGGa                                                                                             >  8:262639/1‑136 (MQ=255)
                                         gctGTACCTGGACACGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCGCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCa                                                                                          <  1:159334/136‑1 (MQ=255)
                                          ctGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCAt                                                                                         <  5:323963/136‑1 (MQ=255)
                                                    acacGGGCGTGGACCTGCGCGAGTTAACGGGGGTGGCCAAGCGCCTCCGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTg                                                                               <  6:227676/136‑1 (MQ=255)
                                                        gggCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCCCCACACCGTGCCGGCCGCCGACCCCGCGGCGCTGGCCTTACAGGCTGATAACCCGGAGCTGCAGCGGCGGGACGGCTGACCAAGATAGGCCAGGATc                                                                           >  2:32641/1‑136 (MQ=255)
                                                          gCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCCCCGCAGCGTGCCCGCCGCCGCCGCCCCGGCGCGGGGCGTGCTGGGTGATCCCCCGGAGCTGCACCGGCGGGGCGGCGGACCATGATAGGCCGGGATagc                                                                         >  4:155502/1‑136 (MQ=255)
                                                                aCCTGCGCGAGTTCAAGGTGGTGGCCCAGCCCCGCAGCGTGCCCGCCCCCCCCGCCGCGGCGCGGGCCGTACGGGCGGGTAACCCAGAGCGGCAGCGGCGGGGCGGCGGACCAAGTACGGCCAGGATcgcccccaa                                                                   >  4:110806/1‑131 (MQ=255)
                                                                     cgcgATTTCAAGGTGGTGGCCAAGCGCCTCAGGGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCg                                                               <  6:367326/135‑1 (MQ=255)
                                                                     cgcgAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCg                                                               <  7:289882/135‑1 (MQ=255)
                                                                     cgcgAGTTCAAGGTGGTGGCCAAGCGCCACAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCg                                                               <  7:92972/135‑1 (MQ=255)
                                                                           ttCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTa                                                         <  8:306297/135‑1 (MQ=255)
                                                                             cAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTAtc                                                       <  6:326588/135‑1 (MQ=255)
                                                                                  tggtggCCAAGCGCCTCAGCGTGCCGGCCGCCGCCGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCGGGCCGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGc                                                 >  3:397277/1‑136 (MQ=255)
                                                                                    gtggCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCt                                               <  1:220379/136‑1 (MQ=255)
                                                                                    gtggCCAAGCGCCCCAGCGGGCGGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCt                                               <  5:44744/136‑1 (MQ=255)
                                                                                        ccAAGCGCCTCAGCGTGCCCGCCGCCGACGCCGAGGCGCTGGCCGTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCGGGATAGCCGCCACGGCGAAGCGATCTCGGGCCGTGAc                                           >  2:220379/1‑136 (MQ=255)
                                                                                        ccAAGCGCCGCAGCGGGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGAc                                           <  4:39917/136‑1 (MQ=255)
                                                                                         cAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGAcc                                          <  3:384261/136‑1 (MQ=255)
                                                                                           aGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGACCAc                                        <  4:96071/136‑1 (MQ=255)
                                                                                                  ctccGTGCGGGCGGAGGTGGCCGTGGCGCTGGCCGTCCTGGCGGTTCACCGTGTGCTGCAGCGGCTGGTCGGCTGACCATGATCGGCCTGGATCGCCGCAAGGGCGAAGCTTTCTGGGGCCTTGTCGACTTgcgcg                                 <  2:30714/133‑2 (MQ=255)
                                                                                                    gCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGCGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGACCACTTGGGCCaa                               >  7:364146/1‑136 (MQ=255)
                                                                                                          cggccgACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGACCACTTGCGCCAATCCATc                         <  6:139380/136‑1 (MQ=255)
                                                                                                          cggccgACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGACCACTTGCGCCAATCCATc                         <  2:263376/136‑1 (MQ=255)
                                                                                                             ccgACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGACCACTTGCGCCAATCCATCg                        >  1:340625/1‑134 (MQ=255)
                                                                                                             ccgACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGACCACTTGCGCCAATCCATCg                        >  8:62620/1‑134 (MQ=255)
                                                                                                                acgacgCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGACCACTTGCGCCAATCCATCGAAGAc                   <  6:339349/136‑1 (MQ=255)
                                                                                                                    cgcctcggCTCTGGCCTTCCTGGCTGCTCACCCTGAGCTGCATCGGCTGGACGGCTGAACATGATCGTCCTGTATCGCCGCAACGGCGAAGCTATCTCGGTCCTTGACCACTTGCGCCAATCCATCTAATACATCt               <  2:103065/130‑1 (MQ=255)
                                                                                                                              cTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGACCACTTGCGCCAATCCATCGAAGACATCTTGACCACGcc     <  7:320967/136‑1 (MQ=255)
                                                                                                                                 gCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGACCACTTGCGCCAATCCATCGAAGACATCTTGACCACGCCACt  >  8:254212/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                                    
GCACAAGTCACTTTCGCCGACACCGTCTACACCTCGCGCTCGCTGTACCTGGACAAGGGCGAGGACCTGCGCGAGTTCAAGGTGGTGGCCAAGCGCCTCAGCGTGCCGGCCGACGACGCCGAGGCGCTGGCCTTCCTGGCTGATCACCCTGAGCTGCAGCGGCTGGACGGCTGACCATGATCGGCCTGGATCGCCGCAACGGCGAAGCTATCTCGGGCCTTGACCACTTGCGCCAATCCATCGAAGACATCTTGACCACGCCACT  >  ACB143/3427630‑3427894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑