Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	3,861,137	C→A	6.7%	R142R (CGC→CGA)	CJ019_03659 →	Gluconokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	3,861,137	0	C	A	6.7%	190.5 / 3.0	60	R142R (CGC→CGA)	CJ019_03659	Gluconokinase
Reads supporting (aligned to +/- strand):  ref base C (19/37);  new base A (2/2);  total (21/39)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01

CCATGCCCGGGCTGGTGTTCGTGTTCCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCGCCTGACCTCGGCGGCATTTCCGTCTC  >  ACB143/3861004‑3861272                                                                                                                                      |                                                                                                                                        ccatgccCGGGCTGGTGTTCGTGTTCCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACgcgg                                                                                                                                       <  7:373191/136‑1 (MQ=255) ccatgccCGGGCTGGTGTTCGTGTTCCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACgcgg                                                                                                                                       <  5:330064/136‑1 (MQ=255)  catgccCGGGCTGGTGTTCGTGTTCCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCCCgcggc                                                                                                                                      >  2:147903/1‑136 (MQ=255)   atgccCGGGCTGGTGTTCGTGTTCCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACgcggcg                                                                                                                                     >  2:256552/1‑136 (MQ=255)   atgccCGGGCTGGTGTTCGTGTTCCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACgcggc                                                                                                                                      >  8:286429/1‑135 (MQ=255)      cccGGGCTGGTGTTCGTGTTCCTCGAACTGTCGCCTGCCGTGGCCGAAATAGGCGTGCTGGCTCGCGCAGGGCACTTCATGCCGTCCAGCCTCATCGACAGCCAGTTCGGCGGCGTGGAATCGCCACGCGGCGAg                                                                                                                                   <  6:84995/135‑1 (MQ=255)       ccGGGCTGGTGTTCGTGTTCCTCGAACTGTCGCCTGCCGAGGCCGAAACACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCCCGCGGCGAGcc                                                                                                                                 >  3:36811/1‑136 (MQ=255)       ccGGGCTGGTGTTCGTGTTCCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGcc                                                                                                                                 <  5:54266/136‑1 (MQ=255)       ccGGGCTGGTGTTCGTGTTCCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGcc                                                                                                                                 <  1:215771/136‑1 (MQ=255)       ccGGGCTGGTGTTCGTGTTCCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGc                                                                                                                                  >  7:337871/1‑135 (MQ=255)       ccGGGCTGGTGTTCGTGTGCCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCGCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGcc                                                                                                                                 <  1:146155/136‑1 (MQ=255)            cTGGTGTTCGTGTTCCTCGAACTGGCGCCTGCCTAGGCCGAAAACCGCGGTCTGGCTCGCCCAGGGCACTTCAGGCCGGCCAGCCTCATCTACAGCAAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGa                                                                                                                            <  8:148657/136‑1 (MQ=255)              ggTGTTCGTGTTCCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGaca                                                                                                                          >  7:240451/1‑136 (MQ=255)                        ttcCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTg                                                                                                                 <  3:187072/135‑1 (MQ=255)                                            cGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCTACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGaa                                                                                            <  7:102700/136‑1 (MQ=255)                                            cGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGaa                                                                                            <  2:162624/136‑1 (MQ=255)                                              aGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAg                                                                                           >  8:286921/1‑135 (MQ=255)                                                 ccGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACt                                                                                       >  1:387590/1‑136 (MQ=255)                                                   gAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTgg                                                                                     >  4:385969/1‑136 (MQ=255)                                                               cTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGAGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGaa                                                                                            <  7:95236/117‑1 (MQ=255)                                                               cTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGAGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGaa                                                                                            >  8:95236/1‑117 (MQ=255)                                                               cTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGAGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGaa                                                                                            <  7:89595/117‑1 (MQ=255)                                                               cTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGAGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGaa                                                                                            >  8:89595/1‑117 (MQ=255)                                                                tGGCTCGCCCAGGGCACTTCATGCCGGCGAGCCTCATCGACAGCCAGTGCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGa                                                                        <  5:85066/136‑1 (MQ=255)                                                                tGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGa                                                                        <  6:186516/136‑1 (MQ=255)                                                                gggCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCGAACCGATCGAAGCACTGGCCGAAGCGGTGGa                                                                        <  5:272746/135‑1 (MQ=255)                                                                  gCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGa                                                                        <  3:276012/134‑1 (MQ=255)                                                                  gCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGa                                                                        <  7:131278/134‑1 (MQ=255)                                                                  gCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGAcc                                                                      <  1:40830/136‑1 (MQ=255)                                                                  gCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGAcc                                                                      <  5:109654/136‑1 (MQ=255)                                                                  gCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTGCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGa                                                                        <  7:241931/134‑1 (MQ=255)                                                                  gCTCGCCCAGGGCACTTCATGCCAGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGa                                                                        <  6:65501/134‑1 (MQ=255)                                                                       cccTGGGCACTTCATGCGGGCAAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTgg                                                                 <  7:156412/136‑1 (MQ=255)                                                                       cccAGGGCACTTCATGCCGGCCAGCCTCATCGCCAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCTCTGGCGGAAGCGGTGGACCACTgg                                                                 <  4:85186/136‑1 (MQ=255)                                                                       cccAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTgg                                                                 <  4:36408/136‑1 (MQ=255)                                                                       cccAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTgg                                                                 <  4:63442/136‑1 (MQ=255)                                                                         cAGGGCACTTCATGCCGGCCAGCCTCATCGACACCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCt                                                               <  5:207066/136‑1 (MQ=255)                                                                            ggCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTaaa                                                            <  8:52475/136‑1 (MQ=255)                                                                            ggCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTaaa                                                            <  6:21260/136‑1 (MQ=255)                                                                               aCTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCa                                                         >  8:98375/1‑136 (MQ=255)                                                                               aCTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCa                                                         >  4:342884/1‑136 (MQ=255)                                                                                   cATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACt                                                       <  5:164711/134‑1 (MQ=255)                                                                                    aTGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCCACCGATCGAAGCACTGGCCGAAGCGGTGGCCCACTGGCTAAAGCACTGCg                                                    >  3:240210/1‑136 (MQ=255)                                                                                             aGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGCCCACTGGCTAAAGCACTGCGGTGAGCCgg                                           >  6:51865/1‑136 (MQ=255)                                                                                              gCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACTCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCggg                                          >  6:110374/1‑136 (MQ=255)                                                                                              gCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCa                                                                                                      >  6:293051/1‑76 (MQ=255)                                                                                              gCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCa                                                                                                      <  5:293051/76‑1 (MQ=255)                                                                                              gCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGa                                                                        >  4:273570/1‑106 (MQ=255)                                                                                              gCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGa                                                                        <  3:273570/106‑1 (MQ=255)                                                                                              gCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCggg                                          >  5:202363/1‑136 (MQ=255)                                                                                                    tCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGgc                                    <  5:144799/136‑1 (MQ=255)                                                                                                       aCAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCg                                                                             >  5:7621/1‑92 (MQ=255)                                                                                                       aCAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCg                                                                             <  6:7621/92‑1 (MQ=255)                                                                                                            ctgggcgcgGCCCTGGAATCGCCACGCGTCGAGCCACTGACACTTGCACTGGACGCCACCCTTCCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCg                            <  2:394211/131‑1 (MQ=255)                                                                                                            cAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCg                            <  6:113093/136‑1 (MQ=255)                                                                                                            cAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCg                            >  2:170069/1‑136 (MQ=255)                                                                                                              gTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACAGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCGcc                          >  6:319376/1‑136 (MQ=255)                                                                                                                  gcgGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCGCCTGAc                      >  5:121546/1‑136 (MQ=255)                                                                                                                    ggCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCGCCTGACCt                    <  1:397554/136‑1 (MQ=255)                                                                                                                                 ccACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCGCCTGACCTCGGCGGCATTTcc       <  7:286429/136‑1 (MQ=255)                                                                                                                                 ccACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCGCCTGACCTCGGCGGCATTTcc       <  8:123407/136‑1 (MQ=255)                                                                                                                                 ccACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCGCCTGACCTCGGCGGCATTTcc       <  2:76774/136‑1 (MQ=255)                                                                                                                                 ccACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCGCCTGACCTCGGCGGCATTTcc       <  8:325117/136‑1 (MQ=255)                                                                                                                                 ccACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCGCCTGACCTCGGCGGCATTTcc       <  1:16444/136‑1 (MQ=255)                                                                                                                                   aCGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCGCCTGACCTCGGCGGCATTTCCGt     >  6:332680/1‑136 (MQ=255)                                                                                                                                      cggcgAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCGCCTGACCTCGGCGGCATTTCCGtctc  <  7:29278/136‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        CCATGCCCGGGCTGGTGTTCGTGTTCCTCGAACTGTCGCCTGCCGAGGCCGAAAAACGCGTGCTGGCTCGCCCAGGGCACTTCATGCCGGCCAGCCTCATCGACAGCCAGTTCGCGGCCCTGGAATCGCCACGCGGCGAGCCACTGACACTTGCACTGGACGCCACCCAACCGATCGAAGCACTGGCCGAAGCGGTGGACCACTGGCTAAAGCACTGCGGTGAGCCGGGCCTGGCGCAAACCGCCTGACCTCGGCGGCATTTCCGTCTC  >  ACB143/3861004‑3861272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑