Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,091,127 C→T 7.0% R59R (CGG→CGA)  dmlR_15 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,091,1270CT 7.0% 188.6 / 3.5 57R59R (CGG→CGA) dmlR_15HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (19/34);  new base T (2/2);  total (21/36)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

GCAACGCCAGACGCAGACGCCCACGAACCGTTGCCGCACCTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGCCCTGCTCATACAAGGCCAGCAGG  >  ACB143/4090994‑4091262
                                                                                                                                     |                                                                                                                                       
gCAACGCCAGACGCAGACGCCCACGAACCGTTGCCGCACCTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCcg                                                                                                                                       >  7:182724/1‑136 (MQ=255)
 cAACGCCAGACGCAGACGCCCACGAACCGTTGCCGCACCTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCcgc                                                                                                                                      <  8:182724/136‑1 (MQ=255)
 cAACGCCAGACGCAGACGCCCACGAACCGTTGCCGCACCTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCcgc                                                                                                                                      <  4:232722/136‑1 (MQ=255)
 cAACGCCAGACGCAGACGCCCACGAACCGGTGCCGCACCTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCcgc                                                                                                                                      <  2:401360/136‑1 (MQ=255)
      ccAGACGCAGACGCCCACGAACCGTTGCCGCACCTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACt                                                                                                                                 <  4:146319/136‑1 (MQ=255)
       cagacgcagacgCCCACGAACCGTTGCCGCACCTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTg                                                                                                                                >  6:360862/1‑136 (MQ=255)
                              ttGCCGCACCTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAg                                                                                                         <  8:229785/136‑1 (MQ=255)
                              tagCCGCACCTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAg                                                                                                         <  6:337267/134‑1 (MQ=255)
                                 ccGCACCTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCcg                                                                                                      <  3:388312/136‑1 (MQ=255)
                                       cTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGTCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGtctc                                                                                                >  6:355605/1‑136 (MQ=255)
                                       cTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGTCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGtctc                                                                                                >  6:361463/1‑136 (MQ=255)
                                         tCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTc                                                                                                                           <  5:388769/107‑1 (MQ=255)
                                         tCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTc                                                                                                                           >  6:388769/1‑107 (MQ=255)
                                         tCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCCCCCGGCGAATGCCCAGCCGCGTctct                                                                                               >  5:114403/1‑135 (MQ=255)
                                            gCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAg                                                                                           >  3:288675/1‑136 (MQ=255)
                                            gCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAg                                                                                           >  6:313983/1‑136 (MQ=255)
                                                ccTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCAc                                                                                        <  8:217582/135‑1 (MQ=255)
                                                   ctTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGAAAAGCGCAGCTGCCGCCTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCCCTCAg                                                                                    >  6:25000/1‑136 (MQ=255)
                                                    ttCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCa                                                                                     <  8:74851/134‑1 (MQ=255)
                                                    ttCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAgg                                                                                   >  7:313134/1‑136 (MQ=255)
                                                    ttCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAgg                                                                                   <  4:247898/136‑1 (MQ=255)
                                                      cctccGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAgg                                                                                   <  7:319037/134‑1 (MQ=255)
                                                      cctccGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAgg                                                                                   <  6:192777/134‑1 (MQ=255)
                                                      cctccGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCACATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAgg                                                                                   <  6:184300/134‑1 (MQ=255)
                                                           gCTTCGGGAATCAGCCGGCTATCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGAGTACTGCGCTCCACCAGGCGAATGCCCAGCCTCGTCTCTAAATCACTCAGGCGTTTCg                                                                            <  7:333188/136‑1 (MQ=255)
                                                            cTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGTCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGa                                                                           <  5:361463/136‑1 (MQ=255)
                                                            cTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGTCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGa                                                                           <  5:355605/136‑1 (MQ=255)
                                                            cTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGa                                                                           <  1:276526/136‑1 (MQ=255)
                                                            cTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGa                                                                           <  4:51245/136‑1 (MQ=255)
                                                            cTTCGGCAATCAGCCGGCTAGCCTAGAGAAGCTTGGCGACCAGCCGCTCGCCCTCATCGGTAAAGCTCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGa                                                                           <  2:166326/136‑1 (MQ=255)
                                                               cGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCTTCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACa                                                                         <  3:179500/135‑1 (MQ=255)
                                                               cGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAg                                                                        >  8:249218/1‑136 (MQ=255)
                                                               cGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGGTTCGACAg                                                                        >  3:286240/1‑136 (MQ=255)
                                                               cGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGAGTTTCGACAg                                                                        >  3:280687/1‑136 (MQ=255)
                                                                 gCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCa                                                                      >  4:332662/1‑136 (MQ=255)
                                                                  cAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCAc                                                                     >  7:27406/1‑136 (MQ=255)
                                                                       aGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGCCAGCACCGTAg                                                                >  6:319462/1‑136 (MQ=255)
                                                                                                   ccAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCcggcgg                                     <  3:99920/135‑1 (MQ=255)
                                                                                                       ccGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGgcagca                                <  2:315124/136‑1 (MQ=255)
                                                                                                       ccGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGgcagca                                <  6:244357/136‑1 (MQ=255)
                                                                                                            cGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCCGGCGTTTCGACAGCACAGTAGGGTGGCGATCCAGTTGCCGCCCGGCGGCAGCAAACGa                           <  6:266703/136‑1 (MQ=255)
                                                                                                            cGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGa                           <  1:369142/136‑1 (MQ=255)
                                                                                                            cGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGa                           <  3:815/136‑1 (MQ=255)
                                                                                                            cGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGGAGGGTGGCCCTCCAGTTGCCGCCCGGCGGCAGCAAACGa                           >  8:82231/1‑136 (MQ=255)
                                                                                                             gCCCTCTTCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTATATCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGa                           <  6:319679/135‑1 (MQ=255)
                                                                                                             gCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGa                           <  1:287002/135‑1 (MQ=255)
                                                                                                             gCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGa                           <  4:245861/135‑1 (MQ=255)
                                                                                                               ccTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGcc                        <  8:74260/136‑1 (MQ=255)
                                                                                                               ccTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGcc                        <  8:89551/136‑1 (MQ=255)
                                                                                                                   aTCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTAGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGCCCt                      <  5:58237/134‑1 (MQ=255)
                                                                                                                        tAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGCCCTGCTCATa               <  1:160532/136‑1 (MQ=255)
                                                                                                                              gcAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGCCCTGCTCATACAAGGc         <  4:83051/136‑1 (MQ=255)
                                                                                                                               cAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACACAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGCCCTGCTCATACAAGGcc        <  5:313983/136‑1 (MQ=255)
                                                                                                                                aGCTGCCGCGTACTGCGCTCCCCCAGGCGAATGCCCAGCCGCGTCTCTAAAGCCCGCAGGCGGTTCGCCAGCCCCGTAGGGTGGCGCTCCATTTGCCGCCCGGCGGCAGCAAACCACCCCTGCGCAGCCAAGGccc       >  8:334260/1‑135 (MQ=255)
                                                                                                                                aGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTGAGGAGTTTCGACAGCACAGTAGGGTGGCGCTGAAGTTGCCGCCCGGCGGCAGCAAACGAGACGTGCTCATACAAGGc         >  8:329096/1‑134 (MQ=255)
                                                                                                                                aGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGCCCTGCTCATACAAGGc         >  5:343820/1‑134 (MQ=255)
                                                                                                                                  cTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCCGCAAACGAGCCCTTCTGATACAGCGCcagc     >  6:322378/1‑136 (MQ=255)
                                                                                                                                     ccGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGCCCTGCTCATACAAGGCCCGCAgg  >  2:266099/1‑136 (MQ=255)
                                                                                                                                     ccGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGCCCTGCTCATACAAGGCCAGCAgg  >  2:360480/1‑136 (MQ=255)
                                                                                                                                     ccGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGCCCTGCTCATACAAGGCCAGCAgg  >  4:183086/1‑136 (MQ=255)
                                                                                                                                     ccGCGTACTGCGATCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTATCGACAGCACCCTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGCCCTGCTCATACAAGCCCAGCAgg  >  2:221424/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GCAACGCCAGACGCAGACGCCCACGAACCGTTGCCGCACCTTCGGCTGCCTCTTCCTCCGCTTCGGCAATCAGCCGGCTAGCCTCGAGAACCTTGGCTACCAGCCGCTCGCCCTCATCGGTAAAGCGCAGCTGCCGCGTACTGCGCTCCACCAGGCGAATGCCCAGCCGCGTCTCTAAAGCACTCAGGCGTTTCGACAGCACCGTAGGGTGGCGCTCCAGTTGCCGCCCGGCGGCAGCAAACGAGCCCTGCTCATACAAGGCCAGCAGG  >  ACB143/4090994‑4091262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑