Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,190,676 G→A 100% intergenic (+890/+101) CJ019_03940 → / ← CJ019_03941 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,190,6760GA100.0% 187.0 / ‑6.8 55intergenic (+890/+101)CJ019_03940/CJ019_03941hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (26/27);  minor base C (1/0);  total (28/27)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATCGGATAGCTATCAGGGGCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGGCAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCTAGACT  >  ACB143/4190554‑4190802
                                                                                                                          |                                                                                                                              
aaTCGGATAGCTATCAGGGGCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCtt                                                                                                                   >  6:277145/1‑136 (MQ=255)
        aGCTATCAGGGGCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTg                                                                                                           <  3:270007/136‑1 (MQ=255)
                ggggCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTc                                                                                                   <  4:214339/136‑1 (MQ=255)
                ggggCGATGCCCGAAGCCTAAAATTCAATCAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTc                                                                                                   <  7:154232/136‑1 (MQ=255)
                 gggCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCg                                                                                                  <  8:196087/136‑1 (MQ=255)
                  ggCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTc                                                                                                   >  7:372144/1‑134 (MQ=255)
                       tgaccGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTAGAGTAg                                                                                             >  6:88167/4‑135 (MQ=255)
                       tGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGCCAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAg                                                                                             >  4:273852/1‑135 (MQ=255)
                       tGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAg                                                                                             >  7:124841/1‑135 (MQ=255)
                       tGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAg                                                                                             >  2:344500/1‑135 (MQ=255)
                       tGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAg                                                                                             >  4:224667/1‑135 (MQ=255)
                       tGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCT‑CACAGACAGGTCCAAGCTTCGAATAGGGAATACTAGAGTAGaa                                                                                           >  5:244965/1‑136 (MQ=255)
                       tGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGATCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCGGGAATACTCGAGTAg                                                                                             >  2:395769/1‑135 (MQ=255)
                        gCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGaa                                                                                           >  2:266157/1‑136 (MQ=255)
                          ccGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGACACACGTTCCAAGATCGCCCCCTCCACAGACAGTACCAAGCTACGAATCAGGAATACTCGATTAGatt                                                                                          >  8:152453/1‑133 (MQ=255)
                                     aaTTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCAAAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAg                                                                              <  8:356063/136‑1 (MQ=255)
                                             gaagaatgaagGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGAGAGGACGGc                                                                        >  1:110420/1‑134 (MQ=255)
                                                           cTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTcgc                                                        <  2:29472/136‑1 (MQ=255)
                                                              cctttcctGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGaa                                                                                           >  7:673/1‑98 (MQ=255)
                                                              cctttcctGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGaa                                                                                           <  8:673/98‑1 (MQ=255)
                                                              cctttcctGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGAt                                                     >  4:184563/1‑136 (MQ=255)
                                                                  tcctGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGa                                                  <  1:410236/135‑1 (MQ=255)
                                                                  tcctGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGa                                                  <  3:383655/135‑1 (MQ=255)
                                                                    ctGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGc                                               <  2:1027/136‑1 (MQ=255)
                                                                       tCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGt                                            >  2:305154/1‑136 (MQ=255)
                                                                         cTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTc                                           >  3:141684/1‑135 (MQ=255)
                                                                            ggTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTAt                                        <  6:87869/135‑1 (MQ=255)
                                                                               cTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTAc                                    >  1:399704/1‑136 (MQ=255)
                                                                               cTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTAc                                    >  4:191634/1‑136 (MQ=255)
                                                                                  ccGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCTAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTAc                                    <  7:326029/133‑1 (MQ=255)
                                                                                   cGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTaaa                                >  4:220494/1‑136 (MQ=255)
                                                                                    ggATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTaaa                                >  8:72671/1‑135 (MQ=255)
                                                                                    ggATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTaaa                                >  7:21625/1‑135 (MQ=255)
                                                                                    ggATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTaaa                                >  3:277373/1‑135 (MQ=255)
                                                                                    ggATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTaaa                                >  3:105710/1‑135 (MQ=255)
                                                                                    ggATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTaaa                                >  4:135553/1‑135 (MQ=255)
                                                                                         cGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGc                           <  1:103744/135‑1 (MQ=255)
                                                                                                acGTTCCAAGATCGCCCCCTCCACAGACAGATCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTagccagc                   <  3:113911/136‑1 (MQ=255)
                                                                                                  gTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATGGAAGCGGTCTATTTACTAAATACGCTAgccagcca                 <  1:69542/136‑1 (MQ=255)
                                                                                                  gTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAgccagcca                 <  3:224667/136‑1 (MQ=255)
                                                                                                  gTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAgccagcca                 <  8:225931/136‑1 (MQ=255)
                                                                                                  gTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAgccagcca                 <  6:351251/136‑1 (MQ=255)
                                                                                                  gTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAgccagcca                 <  2:148203/136‑1 (MQ=255)
                                                                                                  gTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAgccagcca                 <  6:99892/136‑1 (MQ=255)
                                                                                                  gTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAgccagcca                 <  4:278631/136‑1 (MQ=255)
                                                                                                   ttCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAgccagcca                 >  5:81999/1‑135 (MQ=255)
                                                                                                   ttCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCaa                <  1:6843/136‑1 (MQ=255)
                                                                                                        aGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCATACCAc           <  3:270929/136‑1 (MQ=255)
                                                                                                        aGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCAc           <  1:62408/136‑1 (MQ=255)
                                                                                                          aTCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACtt         <  2:246157/136‑1 (MQ=255)
                                                                                                                 ccTCCACAGGCAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGCCGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCTAGACt  >  7:143985/1‑136 (MQ=255)
                                                                                                                 ccTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCTAGACt  <  1:266157/136‑1 (MQ=255)
                                                                                                                 ccTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCTAGACt  >  1:69835/1‑136 (MQ=255)
                                                                                                                 ccTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCTAGACt  <  2:90517/136‑1 (MQ=255)
                                                                                                                 ccTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCAGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACAACGTCTATACt  >  6:271000/1‑136 (MQ=255)
                                                                                                                          |                                                                                                                              
AATCGGATAGCTATCAGGGGCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGGCAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCTAGACT  >  ACB143/4190554‑4190802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑