Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,245,128 A→C 38.7% L178R (CTG→CGG)  CJ019_03982 ← Crotonyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,245,1280AC38.7% 12.3 / 14.4 53L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (14/14);  new base C (16/3);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 2.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

GGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGAAGC  >  ACB143/4244995‑4245263
                                                                                                                                      |                                                                                                                                       
ggCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCCGCCTTAGCTGACCCACCCGGCG‑GCCCgg                                                                                                                                       >  1:178231/1‑136 (MQ=255)
   gcCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCCACCCGGCG‑GCCCGGgcg                                                                                                                                    >  1:276480/1‑136 (MQ=255)
    ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGCCCCACCCGGCG‑GCCCGGgcgc                                                                                                                                   >  2:230091/1‑136 (MQ=255)
    ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGCG‑GCCCGGgggc                                                                                                                                   >  7:55289/1‑136 (MQ=255)
    ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCAGTGGCCCGGCGCCCGGCCAGTGCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCCGACTTCGCTGGCCCACCCGGCG‑GCCCgggggg                                                                                                                                   >  5:215124/1‑133 (MQ=255)
        cgcgTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACGTCGCTGACCAACCCGGCG‑GCCCGGGCGCCATc                                                                                                                               >  1:245530/1‑136 (MQ=255)
             aTCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGACCCACCCGGCG‑GCCCCGGCGCGCTCGgggc                                                                                                                          >  7:326242/1‑136 (MQ=255)
                        gggcTGCGTATTGGGGACGGGGGGGCCATGGGCCTGGCGCCGGGCCAGTTCGGCGGCAACGGCTGGCAACTGGGCGGCGGGGCATACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACtcg                                                                                                               <  8:156735/133‑1 (MQ=255)
                         gTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGACAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GACAGGGCGCCCTCGGCGCCCCACAACacgt                                                                                                              >  6:214812/1‑136 (MQ=255)
                            tGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGa                                                                                                           <  7:76909/136‑1 (MQ=255)
                              cgcATAGGCGACGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCTATCGGCGCCCCACAACtcgtcg                                                                                                            <  3:182767/133‑1 (MQ=255)
                               gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGACCAACCCGGCG‑GCCCCGGCCCGATCGGCGCCCCACAACTCGTCCAgg                                                                                                         >  7:45093/1‑135 (MQ=255)
                               gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCCCAACGCGTCGAgg                                                                                                         >  1:51905/1‑135 (MQ=255)
                                 aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGaa                                                                                                       >  5:115809/1‑135 (MQ=255)
                                 aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGGTCGGCGCCCCCCAACTCGGGCAGGAAc                                                                                                      >  2:377749/1‑136 (MQ=255)
                                  tcggCGAAGGTCGGGCCATTGGCCCGGCGCCCGGCCAGTGCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                                     <  2:125937/134‑1 (MQ=255)
                                  tAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                                     <  6:115809/136‑1 (MQ=255)
                                   agggtaaGGTGGGGCCTTTGGCCTGGCGCCCGGCCTGTTCGGCGGAAACGGCTGGCAACTGCTGGTCGGCGCAGTGTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                                     <  8:156583/130‑1 (MQ=255)
                                      cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAcagcag                                                                                                 >  1:342066/1‑136 (MQ=255)
                                           gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCCGGCGCt                                                                                            >  5:137940/1‑136 (MQ=255)
                                            tCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCtt                                                                                           <  7:115236/136‑1 (MQ=255)
                                                 ccttgggCCAGGCGCCCGGCCAGTGCGGCGGCAACGGCAGTCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCt                                                                                      <  2:71678/131‑1 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCCGGGCGCGCTCGGCGCCCCCCACCGCGGCGCGGGACCGCAGGCGCGTGGccgg                                                                                     >  5:276105/1‑134 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTg                                                                                     >  5:96185/1‑136 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCCGGGCGCGAACGGCGCCCCACACCACGGCGAGGAACAGCAAGCGCTTTGccag                                                                                     >  8:338970/1‑134 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGAACGGCGCCCCACCACTCGTCGAGGAACAGCAGGCGCTTGGCCTg                                                                                     >  4:368110/1‑136 (MQ=255)
                                                    ttGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCGCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCTGGGCGCTATGGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCt                                                                                   <  7:217436/136‑1 (MQ=255)
                                                        ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCATAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCt                                                                               >  1:28279/1‑136 (MQ=255)
                                                        ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCt                                                                               >  2:2881/1‑136 (MQ=255)
                                                             cgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCCGGCGCTTGGCCTGCTCGCTGCCgc                                                                          >  7:84444/1‑135 (MQ=255)
                                                               cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGAt                                                                         >  7:47751/1‑135 (MQ=255)
                                                                     cAGTTCGGCGGCAACGGCCGCCAACTGCTCGTCGGCGCAGACTTCGCTCACCACCCCGGCG‑GCCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                                  <  8:216598/136‑1 (MQ=255)
                                                                     cAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCATACTTCGCTGACCACCCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                                  <  6:395633/136‑1 (MQ=255)
                                                                                cAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCCGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCATGGCCTGCTCGCTGCCGAACAGCCGTGGCAGGTGCCa                                                       >  4:219050/1‑136 (MQ=255)
                                                                                cAACGCCAGGCAACTGCTCGTCGGCGCTGACTGCGCTGACCAACCCGGCG‑GCCGGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCa                                                       <  6:344360/136‑1 (MQ=255)
                                                                                    ggcaggcaACTGCTCGGCGGCGCAGACTGCGCTGACCACCCCGGCG‑GCCTGGGCGCGAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTg                                                   <  4:302882/136‑1 (MQ=255)
                                                                                     gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTgg                                                  >  4:393323/1‑136 (MQ=255)
                                                                                     gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCACGGCGCGGTCGGCGCCCCACAACTGGTCGAGGACCAGCAGGCGCGTGGCCAGCTCGCTGGCGCTCAGCCGGTGCAGGTGCCCGCTgg                                                  >  3:7997/1‑136 (MQ=255)
                                                                                      ccggcaACTGCGCGGCGGCGCAGACTTCGCTCCCCACCCCGGCG‑GCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                                 <  2:178231/134‑1 (MQ=255)
                                                                                      ccgccaACTGCGCGGCGGCGCACACTGCGCCCACCACCCCGGCG‑GCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                                 <  5:36233/132‑1 (MQ=255)
                                                                                      caggaaACTGCGGGGGGGCCCAGACTTCGCTGACCAACCCGGCG‑GCCTGGGCGCGAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                                 <  5:225186/136‑1 (MQ=255)
                                                                                                cgggcgggCGCAGAATGCG‑TGCCCCACCCCGCGCGCCGGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGACCAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCAt                                         <  6:229938/128‑1 (MQ=255)
                                                                                                 tcgtcgGCGCAGACTTCGCTGACCAACCCGGCG‑GCCCGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCCGGCGCTTGGCCAGCTCGCTGCCGATCAGCCGGGGCCGGTGCCCGCTGGCGCCGGCATCgg                                      >  1:92514/1‑136 (MQ=255)
                                                                                                  cggcgGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                                     <  4:281724/133‑1 (MQ=255)
                                                                                                   gtcgGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCATAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                                    <  2:28279/136‑1 (MQ=255)
                                                                                                        cgcAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCCGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTg                                 >  5:377454/1‑134 (MQ=255)
                                                                                                           aGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGTTCGGCGCCCCACAACTCGGCGCGGAACAGCAGGCGCTTTGCCTGCTCGCTGGCGATCAGCCGTGGCAAGTGCCAGCAGGCGCCCGCATCGGGGCTGTAGcc                            >  2:37415/1‑136 (MQ=255)
                                                                                                               ttggCTGACCACCCCGGCG‑GCCCGGGCGGTATCGCCGCCCCACAACTCGTCGAGTAACAGCAGGCGCTTTGCCTGCTCGCTTCCGAGCAGCCTTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATg                        <  8:341024/133‑1 (MQ=255)
                                                                                                                    tGACCAACCCGGCG‑GCCCCGGCGCGATCGGCGCCCCACAACACGTCGCGCAACACCACGCGCGTGGCCAGCTCCCTGCCGCTCACCCGTGGGCGGGGGCAGCGGGCGCCCGCCTCGGGGGGGTTGCCCATggcggg                   >  6:17885/1‑131 (MQ=255)
                                                                                                                     gACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtg                  >  1:223204/1‑136 (MQ=255)
                                                                                                                      accaccCGGGCG‑GCCAGGGGGCTTTCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt                 <  8:22484/136‑1 (MQ=255)
                                                                                                                      aCCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt                 <  6:96185/136‑1 (MQ=255)
                                                                                                                      aCCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt                 <  1:2881/136‑1 (MQ=255)
                                                                                                                                      aGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCGTGAAgc  >  3:57476/1‑136 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCG‑GCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGAAGC  >  ACB143/4244995‑4245263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑