Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	4,325,308	C→T	5.2%	P162P (CCG→CCA)	CJ019_04061 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	4,325,308	0	C	T	5.2%	266.1 / 3.0	77	P162P (CCG→CCA)	CJ019_04061	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/41);  new base T (2/2);  total (34/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.93e-01

GGGTAGCCCAACAGGTGGGCAATACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTGCGCACCGTCTGCAACCGCTGCTGGTAGCTCTTG  >  ACB143/4325174‑4325443                                                                                                                                       |                                                                                                                                        gggTAGCCCAACAGGTGGGCAATACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCg                                                                                                                                        <  8:314122/136‑1 (MQ=255)      gCCCAACAGGTGGGCAATACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGccc                                                                                                                                    <  7:370422/135‑1 (MQ=255)      gCCCAACAGGTGGGCAATACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCccg                                                                                                                                   >  6:152880/1‑136 (MQ=255)      gCCCAACAGGTGGGCAATACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCccg                                                                                                                                   <  6:267443/136‑1 (MQ=255)      gCCCAACAGGTGGGCAATACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCccg                                                                                                                                   >  6:328119/1‑136 (MQ=255)      gCCCAACAGGTGGGCAATAACGAAGTACATGCAGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGAAGGGGAAATCCCTCGCAAGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGccct                                                                                                                                   >  7:145507/1‑135 (MQ=255)        ccAACAGGTGGGCAATACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCccgcc                                                                                                                                 <  3:237925/136‑1 (MQ=255)             aGGTGGGCAATACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGgtgt                                                                                                                            <  4:94152/136‑1 (MQ=255)              ggTGGGCAATACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGtgtg                                                                                                                           <  8:59841/136‑1 (MQ=255)               gagggCAATACCGAACTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATAGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCTATGCTGACTTGCATCGGCCCGCCGGtgtg                                                                                                                           >  5:259248/3‑135 (MQ=255)                   gCAATACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCg                                                                                                                      <  6:355109/136‑1 (MQ=255)                    cAATACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCg                                                                                                                      >  1:290694/1‑135 (MQ=255)                     aaTACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGa                                                                                                                    >  2:72124/1‑136 (MQ=255)                       tACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTTACTTCATGGCTGTAGGGGTAATCAATAGCATGCTTTTAGGCAAAATCGATGCTGACTTGCATCGGCCCTCCGGTGTTCACCGGAtt                                                                                                                  >  6:387532/1‑136 (MQ=255)                          cGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAg                                                                                                               >  3:96640/1‑136 (MQ=255)                           gAAGTACATGCCGCCGCGCCTGCTTAACACTTCCTGGGGAATGGTGCCTTCATGGCTGTAGGGGTAATCACTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGGATCGGCCCGCCGGTGTGCACCGGATTCAgc                                                                                                              <  2:114206/136‑1 (MQ=255)                                  aTGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCa                                                                                                       <  8:329837/136‑1 (MQ=255)                                  aTGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCa                                                                                                       <  6:182629/136‑1 (MQ=255)                                  aTGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCa                                                                                                       <  1:408063/136‑1 (MQ=255)                                  aTGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCa                                                                                                       <  5:5359/136‑1 (MQ=255)                                  aTGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCa                                                                                                       <  1:313647/136‑1 (MQ=255)                                   tGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAg                                                                                                      <  5:7210/136‑1 (MQ=255)                                    gccgccGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCCGCCCGCCCAg                                                                                                      >  8:24994/1‑135 (MQ=255)                                       gccgcgccgGCTGAACACTTCCTGGCGAATCGTGCATTCATGTCTGTCGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAg                                                                                                  <  3:330972/136‑1 (MQ=255)                                         cgcgccgGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGt                                                                                                >  6:207961/1‑136 (MQ=255)                                            gccgGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGtgtg                                                                                                                           <  3:341675/106‑1 (MQ=255)                                            gccgGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGtgtg                                                                                                                           >  4:341675/1‑106 (MQ=255)                                            gccgGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCtt                                                                                             >  6:112508/1‑136 (MQ=255)                                            gccgGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCtt                                                                                             >  8:191081/1‑136 (MQ=255)                                            gccgGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCtt                                                                                             >  8:191085/1‑136 (MQ=255)                                                              ggCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTATCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCTGCGGCTGGCGTg                                                                            >  8:367123/1‑135 (MQ=255)                                                               gCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTg                                                                            <  7:357449/134‑1 (MQ=255)                                                                cGAATCGTGCCTTCATGGCTGTATTGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCaa                                                                         <  1:58333/136‑1 (MQ=255)                                                                cGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCaa                                                                         <  6:202891/136‑1 (MQ=255)                                                                cGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCaa                                                                         <  1:78909/136‑1 (MQ=255)                                                                cGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCaa                                                                         <  6:198859/136‑1 (MQ=255)                                                                  aaTCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACg                                                                       <  1:370117/136‑1 (MQ=255)                                                                      gTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACtt                                                                   >  3:47935/1‑136 (MQ=255)                                                                      gTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACt                                                                    <  4:61316/135‑1 (MQ=255)                                                                      gTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACt                                                                    <  7:165442/135‑1 (MQ=255)                                                                          cTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTtcgtc                                                               <  7:146971/136‑1 (MQ=255)                                                                          cTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTtcgtc                                                               <  5:227413/136‑1 (MQ=255)                                                                          cTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTtcgtc                                                               <  5:369918/136‑1 (MQ=255)                                                                          cTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTtcgtc                                                               <  4:231708/136‑1 (MQ=255)                                                                          cTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTtcgtc                                                               <  6:347419/136‑1 (MQ=255)                                                                          cTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTtcgcc                                                               <  8:35909/136‑3 (MQ=255)                                                                             cATGGCTGGAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTcgtcgt                                                             <  1:303760/135‑1 (MQ=255)                                                                                 gCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATTGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGcc                                                                                          <  4:147204/102‑1 (MQ=255)                                                                                 gCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATTGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGcc                                                                                          <  4:153350/102‑1 (MQ=255)                                                                                 gCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATTGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGcc                                                                                          >  3:153350/1‑102 (MQ=255)                                                                                 gCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATTGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGcc                                                                                          >  3:147204/1‑102 (MQ=255)                                                                                 gCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGt                                                        >  4:45776/1‑136 (MQ=255)                                                                                        gggTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTc                                                  >  2:301735/1‑135 (MQ=255)                                                                                                ccTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCg                                                                              <  5:38580/99‑1 (MQ=255)                                                                                                ccTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCg                                                                              >  6:38580/1‑99 (MQ=255)                                                                                                ccTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCttt                                         >  5:158782/1‑136 (MQ=255)                                                                                                ccTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCttt                                         >  8:90780/1‑136 (MQ=255)                                                                                                ccTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCttt                                         >  1:342038/1‑136 (MQ=255)                                                                                                ccTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCttt                                         >  3:233101/1‑136 (MQ=255)                                                                                                ccTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCttt                                         >  4:74408/1‑136 (MQ=255)                                                                                                     cATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCt                                    >  1:203198/1‑136 (MQ=255)                                                                                                      aTGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTg                                   >  8:141938/1‑136 (MQ=255)                                                                                                      aTGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTg                                   >  3:375658/1‑136 (MQ=255)                                                                                                      aTGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTg                                   >  4:147635/1‑136 (MQ=255)                                                                                                      aTGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTg                                   >  4:382372/1‑136 (MQ=255)                                                                                                      aTGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTg                                   >  6:24218/1‑136 (MQ=255)                                                                                                       tGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTgc                                  <  7:191081/136‑1 (MQ=255)                                                                                                       tGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTgc                                  <  7:191085/136‑1 (MQ=255)                                                                                                       tGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTgc                                  <  3:41748/136‑1 (MQ=255)                                                                                                       tGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTgc                                  <  8:251554/136‑1 (MQ=255)                                                                                                       tGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTgc                                  <  2:203198/136‑1 (MQ=255)                                                                                                             tCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTGCGCAcc                             <  2:254724/135‑1 (MQ=255)                                                                                                                  aaaaTCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTGCGCACCGTCTGc                       <  1:142317/136‑1 (MQ=255)                                                                                                                      tCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTGCGCACCGTCTGCAAcc                   <  3:394221/136‑1 (MQ=255)                                                                                                                       cGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTATAGTTCACTCAACTGCTTTTCGCTGCGCACCGTCTGCAACCg                  >  1:92997/1‑136 (MQ=255)                                                                                                                       cGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTGCGCACCGTCTGCAACCg                  >  2:323082/1‑136 (MQ=255)                                                                                                                            cTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTGCGCACCGTCTGCAACCgctgct             <  5:18942/136‑1 (MQ=255)                                                                                                                                       cGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTGCGCACCGTCTGCAACCGCTGCTGGTAGCTCTTg  <  6:34031/136‑1 (MQ=255)                                                                                                                                       cGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTGAGCACCGTCTGCAACCGCTGCTGGTAGCTCTTg  >  4:313704/1‑136 (MQ=255)                                                                                                                                       |                                                                                                                                        GGGTAGCCCAACAGGTGGGCAATACCGAAGTACATGCCGCCGCGCCGGCTGAACACTTCCTGGCGAATCGTGCCTTCATGGCTGTAGGGGTAATCCCTCGCATGCTTTTCGGCAAAATCGATGCTGACTTGCATCGGCCCGCCGGTGTGCACCGGATTCAGCCCGCCCAGCAAGGTCTTGCCCAGCGGCAGGCGTGCAACGACTTCGTCGTACAGTTCACTCAACTGCTTTTCGCTGCGCACCGTCTGCAACCGCTGCTGGTAGCTCTTG  >  ACB143/4325174‑4325443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑