Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 255,173 T→A 5.7% V203E (GTG→GAG)  CJ019_00230 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143255,1730TA 5.7% 216.3 / 4.4 70V203E (GTG→GAG) CJ019_00230hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/34);  new base A (2/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

GCTGGCTCAAGCACCAGCCGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTGGCAGGTACGCCTGCGGG  >  ACB143/255041‑255307
                                                                                                                                    |                                                                                                                                      
gctggctCAAGCACCAGCCGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTtggtgg                                                                                                                                      <  8:157727/135‑1 (MQ=255)
 ctggctCAAGCACCAGCCGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTggcg                                                                                                                                    <  6:335968/136‑1 (MQ=255)
     ctCAAGCACCAGCCGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCac                                                                                                                                <  8:291478/136‑1 (MQ=255)
     ctCAAGCACCAGCCGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGCTGGCGGCac                                                                                                                                >  3:232468/1‑136 (MQ=255)
     ctCAAGCACCAGCCGGCGGTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCac                                                                                                                                <  5:114954/136‑1 (MQ=255)
        aaGCACCAGCCGGCGTTGCTGGCGCTCGCAGCGATCGCCGAGACCGAGCCAGCCACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCactga                                                                                                                             >  4:373315/1‑136 (MQ=255)
          gCACCAGCCGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCTGCactgact                                                                                                                           <  6:363736/136‑1 (MQ=255)
          gCACCAGCCGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCactgact                                                                                                                           <  4:55170/136‑1 (MQ=255)
          gCACCAGCCGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCactgact                                                                                                                           <  4:45346/136‑1 (MQ=255)
          gCACCAGCCGGCGTGGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCactgact                                                                                                                           <  1:394434/136‑1 (MQ=255)
            aCCAGCCGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCActgactga                                                                                                                         >  1:247584/1‑136 (MQ=255)
              cAGCCGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGAcc                                                                                                                       <  4:257214/136‑1 (MQ=255)
              cAGCCGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGAcc                                                                                                                       <  8:172109/136‑1 (MQ=255)
                  cGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGc                                                                                                                   >  6:344193/1‑136 (MQ=255)
                   ggCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGCGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCt                                                                                                                  >  2:254868/1‑136 (MQ=255)
                   ggCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCt                                                                                                                  >  6:287251/1‑136 (MQ=255)
                   ggCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGAGGCGGCACTGACTGACCAGGCt                                                                                                                  >  3:174862/1‑136 (MQ=255)
                   ggCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGAGGCGGCACTGACTGACCAGGCt                                                                                                                  >  3:180929/1‑136 (MQ=255)
                    gCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCtt                                                                                                                 <  1:69218/136‑1 (MQ=255)
                     cGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCAGCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTg                                                                                                                <  4:124451/136‑1 (MQ=255)
                                         cgcGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAgccg                                                                                            <  3:207447/134‑1 (MQ=255)
                                         cTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAgccg                                                                                            <  2:117630/136‑1 (MQ=255)
                                         cTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAgccg                                                                                            <  2:111815/136‑1 (MQ=255)
                                         cTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAgccg                                                                                            <  5:258275/136‑1 (MQ=255)
                                          tCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAgccgc                                                                                           >  2:22912/1‑136 (MQ=255)
                                            gCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCAGcca                                                                                         >  1:265262/1‑136 (MQ=255)
                                              cGAGACCGAGCCAGACACCGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGTCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGccacc                                                                                       <  8:72138/136‑1 (MQ=255)
                                                      aGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAgg                                                                               <  4:186025/136‑1 (MQ=255)
                                                        ccAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCa                                                                             <  8:323223/136‑1 (MQ=255)
                                                        ccAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCa                                                                             <  6:52658/136‑1 (MQ=255)
                                                        ccAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCa                                                                             <  7:170479/136‑1 (MQ=255)
                                                        ccAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCa                                                                             <  2:41052/136‑1 (MQ=255)
                                                        ccAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCa                                                                             <  2:247584/136‑1 (MQ=255)
                                                        ccAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTAGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCa                                                                             <  1:138964/136‑1 (MQ=255)
                                                         cAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCa                                                                             >  8:231037/1‑135 (MQ=255)
                                                             cacaGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGtt                                                                        <  1:255093/136‑1 (MQ=255)
                                                             cacaGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGAAAAGtt                                                                        <  4:235215/136‑1 (MQ=255)
                                                              acaGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTg                                                                       >  1:142883/1‑136 (MQ=255)
                                                                  agGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGAGAGCTCGGTGTTGACTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCa                                                                   <  2:280426/136‑1 (MQ=255)
                                                                  agGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCa                                                                   <  8:70215/136‑1 (MQ=255)
                                                                     tGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCCCATTAGGCAAAGTTGGTCAGGa                                                                >  4:193995/1‑136 (MQ=255)
                                                                     tGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGa                                                                >  6:321532/1‑136 (MQ=255)
                                                                             gcgGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAg                                                        <  3:130688/136‑1 (MQ=255)
                                                                               ggCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGc                                                      >  3:359207/1‑136 (MQ=255)
                                                                                   aTCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCgg                                                  <  5:373799/136‑1 (MQ=255)
                                                                                   aTCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGAGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCgg                                                  <  4:180929/136‑1 (MQ=255)
                                                                                   aTCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGAGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCgg                                                  <  4:174862/136‑1 (MQ=255)
                                                                                          cATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCtggt                                           <  2:347107/136‑1 (MQ=255)
                                                                                              gggCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGAt                                       >  7:211752/1‑136 (MQ=255)
                                                                                                       ccGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGcc                              >  4:132035/1‑136 (MQ=255)
                                                                                                        cGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGcc                              >  2:230613/1‑135 (MQ=255)
                                                                                                        cGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGcc                              >  3:96739/1‑135 (MQ=255)
                                                                                                        cGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGcc                              >  1:340281/1‑135 (MQ=255)
                                                                                                        cGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGcc                              >  5:309417/1‑135 (MQ=255)
                                                                                                        cGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGcc                              >  5:256873/1‑135 (MQ=255)
                                                                                                             cAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAgcc                                                                                             <  4:222002/67‑1 (MQ=255)
                                                                                                             cAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAgcc                                                                                             >  3:222002/1‑67 (MQ=255)
                                                                                                             cAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATgcc                        >  6:24927/1‑134 (MQ=255)
                                                                                                                  cGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAACCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTg                   >  4:391390/1‑136 (MQ=255)
                                                                                                                   ggTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCt                    <  7:193792/134‑1 (MQ=255)
                                                                                                                   ggTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCt                    <  4:232468/134‑1 (MQ=255)
                                                                                                                   ggTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTgg                  >  3:270462/1‑136 (MQ=255)
                                                                                                                   ggTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTgg                  >  1:23673/1‑136 (MQ=255)
                                                                                                                   ggTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTgg                  >  5:380718/1‑136 (MQ=255)
                                                                                                                   ggTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTgg                  >  5:217820/1‑136 (MQ=255)
                                                                                                                   ggTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTgg                  >  4:242792/1‑136 (MQ=255)
                                                                                                                        tGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTGGCAgg              >  8:97684/1‑135 (MQ=255)
                                                                                                                         gCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGACAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCAGATGACTTCTGGCAGGt             <  8:381845/135‑1 (MQ=255)
                                                                                                                             gCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTGGCAGGTACGcc        >  8:235227/1‑136 (MQ=255)
                                                                                                                             gCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTGGCAGGTACGcc        >  6:120918/1‑136 (MQ=255)
                                                                                                                                ttGGTGGCGGCACTGACTGCCCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCTCCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTGGCAGGTACGCCTGc     >  2:319246/1‑136 (MQ=255)
                                                                                                                                 tgttggCGGCACTGACTGACCAGGCTTGGGAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGACCTTGCCGATGACTTCTGGCAGGTACGCCTGCg    <  7:286029/133‑1 (MQ=255)
                                                                                                                                   gtggCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTGGCAGGTACGCCTGCggg  <  8:310630/136‑1 (MQ=255)
                                                                                                                                    tggCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCg                                                   >  3:5713/1‑86 (MQ=255)
                                                                                                                                    tggCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCg                                                   <  4:5713/86‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
GCTGGCTCAAGCACCAGCCGGCGTTGCTGGCGCTGGCAGCGCTCGCCGAGACCGAGCCAGACACAGAGGTGCGCCGCGCGGCCATCGGCGCATTGGGCCTTGCCCGGGACAGCTCGGTGTTGCCTGCGTTGGTGGCGGCACTGACTGACCAGGCTTGGCAAGTGCGCGAGGAAGCCGCCACCACATTAGGCAAAGTTGGTCAGGACCAAGCAGGCCCGGCGCTGGTGGATGCCCTTGCCGATGACTTCTGGCAGGTACGCCTGCGGG  >  ACB143/255041‑255307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑